Utilization of <i>Jatropha curcas</i> L. Leaf Extract Gel for Improving Temperature, Infarct Volume, TNF-Α, and IL-1β in Post-Ischemic Brain Mice

http://www.doi.org/10.26538/tjnpr/v7i12.40

Abstract

This study is motivated by the urgent needs for novel approaches to alleviate the detrimental effects of brain ischemia, a condition marked by reduced blood flow to the brain and severe neurological consequences. The effect of a gel compress made from extracts of Jatropha leaves (Jatropha curcas L) is evaluated on temperature improvement, infarct volume, TNF-α, and IL-1β in a post-brain ischemic mice model. The study employed a pure experimental research design with a Randomized Post Design Test-Only Control Group design. Ischemic conditions in mice were induced through unilateral carotid artery occlusion for 45 minutes, followed by applying Jatropha curcas L leaf extract gel compress. The analysis results indicate that Jatropha curcas L leaf extract gel compress significantly affected temperature improvement, TNF-α, and IL-1β levels (p<0.05). However, no significant effect was observed on infarct volume (p>0.05). Further post hoc analysis revealed that treatment with a 1% concentration and 1.5% concentration of the Jatropha curcas L leaf extract gel compress resulted in a significant temperature drop compared to the control group. Moreover, these concentrations significantly influenced TNF-α levels, while all concentrations significantly improved IL-1β levels. In conclusion, this research demonstrates that a 1.5% concentration of Jatropha curcas L leaf extract gel compress yields the most favorable outcomes regarding temperature improvement, infarct volumes, TNF-α, and IL-1β in a post-brain ischemic mouse model. This study contributes valuable insights into the potential therapeutic applications of Jatropha curcas L leaf extract in mitigating the adverse effects of brain ischemia.