Inhibitory Effects on α-Amylase and α-Glucosidase and Phytochemical Profiling of the Aerial Part of Canna x generalis L.H Bailey & E.Z Bailey http://www.doi.org/10.26538/tjnpr/v7i12.37

Article Sidebar

pdf doi epub
Published: Dec 31, 2023
Keywords:
diabetes, phenolic, flavonoid, Canna x generalis, α-glucosidase, α-amylase

Main Article Content

Nguyen T. Van Anh
University of Science and Technology of Hanoi, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi 10000, Vietnam
Le H. Luyen
University of Science and Technology of Hanoi, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi 10000, Vietnam

Abstract

Canna x generalis L.H Bailey & E.Z Bailey, Cannaceae, has been traditionally used to treat different diseases. However, the scientific evidence about its chemical composition and biological activities is minimal. This study aimed to evaluate for the first time the α-amylase and α-glucosidase inhibitory effects and phytoconstituents of the aerial part of C. generalis. Aerial C. generalis was extracted with ethanol, and then successively fractionated with n-hexane, ethyl acetate and water. The total extract and fractions were evaluated for their α-amylase and α-glucosidase inhibitory effects. The qualitative phytochemicals, the total phenolic content (TPC) and total flavonoid content (TFC) were assessed. The results showed that all extracts showed inhibitory effects against both α-amylase and α-glucosidase in a dose dependent manner. The ethyl acetate fraction exhibited the strongest α-amylase and α-glucosidase inhibitory effects (IC50, 48.0  4.48 and 78.0  4.61 g/mL for the α-amylase and α-glucosidase inhibition assay, respectively, p < 0.05). The ethyl acetate fraction also had the highest TPC and TFC compared to the others (p < 0.05). Moreover, the aerial part of C. generalis contained various phytoconstituents. Therefore, C. generalis, particularly, its ethyl acetate fraction could serve as a promising natural source for searching bioactive compounds that inhibit the two key enzymes, α-amylase and α-glucosidase. In addition, this interesting plant could be a potential candidate for the development of supplementary products for controlling hyperglycemia.

Article Details

How to Cite
Van Anh, N. T., & Luyen, L. H. (2023). Inhibitory Effects on α-Amylase and α-Glucosidase and Phytochemical Profiling of the Aerial Part of Canna x generalis L.H Bailey & E.Z Bailey: http://www.doi.org/10.26538/tjnpr/v7i12.37. Tropical Journal of Natural Product Research (TJNPR), 7(12), 5623-5628. https://tjnpr.org/index.php/home/article/view/3207
Issue
Vol. 7 No. 12 (2023): Tropical Journal of Natural Product Research (TJNPR)
Section
Articles
Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

References

American Association of Diabetes. Diagnosis and classification of diabetes mellitus. Diabetes Care. 2009; 32(1): S62-7.

Salehidoost R, Mansouri A, Amini M, Aminorroaya Yamini S, Aminorroaya A. Diabetes and all-cause mortality, a 18-year follow-up study. Sci Rep. 2020; 10(1): 3183.

Gong L, Feng D, Wang T, Ren Y, Liu Y, Wang J. Inhibitors of α-amylase and α-glucosidase: Potential linkage for whole cereal foods on prevention of hyperglycemia. Food Sci Nutr. 2020; 8(12): 6320-37.

Nguyen TTN, Ngo HLT, Nguyen, TTB, Bui TT. Evaluation of antioxidant and α-glucosidase inhibitory activities of Codonopsis javanica (Blume) Hook. f. Thoms’ root extract. VNU J: Med Pharmaceutical Sci. 2020; 36(3): 57-65.

Bhatia A, Singh B, Arora R, Arora S. In vitro evaluation of the α-glucosidase inhibitory potential of methanolic extracts of traditionally used antidiabetic plants. BMC Complement Altern Med. 2019; 19(1): 74.

Deka H, Choudhury A, Dey BK. An overview on plant derived phenolic compounds and their role in treatment and management of diabetes. J Pharmacopuncture. 2022; 25(3): 199-208.

Srakeaw W, Maneechai S, Katisart T. Antioxidant and α-glucosidase inhibitory activities of Dolichandrone serrulata Extracts. Trop J Nat Prod Res. 2021; 5(6): 1039-43.

Al-Snafi AS. Bioactive components and pharmacological effects of Canna indica - an overview. Int J Pharmacol Toxicol. 2015; 5(2): 71-5.

Vu TH, Le QU. Edible Canna (Canna edulis Ker), a potential crop for Vietnam food industry. Int J Botany Stud. 2019; 4(4): 58-9.

Zhang J, Wang Z-W, Mi Q. Phenolic compounds from Canna edulis Ker residue and their antioxidant activity. LWT - Food Sci Technol. 2011; 44(10): 2091-6.

Le HL, Vu TT, Le TTH, Duong TLH, Nguyen TVA. Inhibitory effect on human platelet aggregation, antioxidant activity, and phytochemicals of Canna warszewiczii (A. Dietr) Nb. tanaka. Pharmacog Res. 2020; 12(1): 47-52.

Nguyen TMH, Le HL, Ha TT, Bui BH, Le NT, Nguyen VH, Nguyen TVA. Inhibitory effect on human platelet aggregation and coagulation and antioxidant activity of C. edulis Ker Gawl rhizome and its secondary metabolites. J Ethnopharmacol. 2020; 263: 113136.

Kayode J. Conservation of indigenous medicinal botanicals in Ekiti State, Nigeria. J Zhejiang Univ Sci B. 2006; 7(9): 713-8.

Xie F, Gong S, Zhang W, Wu J, Wang Z. Potential of lignin from Canna edulis ker residue in the inhibition of α-d-glucosidase: Kinetics and interaction mechanism merging with docking simulation. Int J Biol Macromol. 2017; 95: 592-602.

Mahmoud TN, El-Maadawy WH, Kandil ZA, Khalil H, El-fiky NM, El Alfy TSMA. Canna x generalis L.H. Bailey rhizome extract ameliorates dextran sulfate sodium-induced colitis via modulating intestinal mucosal dysfunction, oxidative stress, inflammation, and TLR4/ NF-ҡB and NLRP3 inflammasome pathways. J Ethnopharmacol. 2021; 269: 113670.

Le HL, Nguyen TMH, Vu TT, Nguyen TTO, Ly HDT, Le NT, Nguyen VH, Nguyen TVA. Potent antiplatelet aggregation, anticoagulant and antioxidant activity of aerial Canna x generalis L.H Bailey & E.Z Bailey and its phytoconstituents. South African J Botany. 2022; 147: 882-93.

Wu YW, Han Y-S, Kim M-H. Antioxidant, α-amylase and α-glucosidase inhibitory activities of Cedrela sinensis (A. Juss) leaf with ethanol extract concentration. Foood Sci Technol. 2022; 42: (e89122).

Zhang J, Zhao S, Yin P, Yan L, Han J, Shi L, Zhou X, Liu Y, Ma C. α-Glucosidase inhibitory activity of polyphenols from the burs of Castanea mollissima Blume. Molecules. 2014; 19(6): 8373-86.

Jagessar R. Phytochemical screening and chromatographic profile of the ethanolic and aqueous extract of Passiflora edulis and Vicia faba L. (Fabaceae). J Pharmacog Phytochem. 2017; 6(6): 1714-21.

Singleton VL, Orthofer R, Lamuela-Raventós RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Meth Enzymol. 1999; 299: 152-78.

Ghauri S, Raza SQ, Imran M, Saeed S, Rashid M, Naseer R. Assessment of α-amylase and α-glucosidase inhibitory potential of Citrus reticulata peel extracts in hyperglycemic/hypoglycemic rats. 3 Biotech. 2021; 11(4): 167.

Takahama U, Hirota S. Interactions of flavonoids with α-amylase and starch slowing down its digestion. Food Funct. 2018; 9(2): 677-87.

Patil SM, Martiz RM, Satish AM, Shbeer AM, Ageel M, Al-Ghorbani M, Ranganatha Lt, Parameswaran S, Ramu R. Discovery of novel coumarin derivatives as potential dual inhibitors against α-glucosidase and α-amylase for the management of post-prandial hyperglycemia via molecular modelling approaches. Molecules. 2022; 27(12): 3888

Khan H, Amin S, Tewari D, Nabavi SM, Atanasov AG. Plant-derived glycosides with α-glucosidase inhibitory activity: current standing and future prospects. Endocr Metab Immune Disord Drug Targets. 2019; 19(4): 391-401.

Vento M, Aguar M, Escobar J, Arduini A, Escrig R, Brugada M, Izquierdo I, Asensi MA, Sastre J, Saenz P, Gimeno A. Antenatal steroids and antioxidant enzyme activity in preterm infants: influence of gender and timing. Antioxid Redox Signal. 2009; 11(12): 2945-55.

Zhang Y, Cai P, Cheng G, Zhang Y. A brief review of phenolic compounds identified from plants: their extraction, analysis, and biological activity. Nat Prod Commun. 2022; 17(1).

Okolie NP, Falodun A, Davids O. Evaluation of the antioxidant activity of root extract of pepper fruit (Dennetia tripetala), and its potential for the inhibition of lipid peroxidation. Afr J Tradit Complement Altern Med. 2014; 11(3): 221-7.

Oboh G, Agunloye OM, Adefegha SA, Akinyemi AJ, Ademiluyi AO. Caffeic and chlorogenic acids inhibit key enzymes linked to type 2 diabetes (in vitro): a comparative study. J Basic Clin Physiol Pharmacol. 2015; 26(2): 165-70.

Oboh G, Ogunsuyi OB, Ogunbadejo MD, Adefegha SA. Influence of gallic acid on α-amylase and α-glucosidase inhibitory properties of acarbose. J Food Drug Anal. 2016; 24(3): 627-34.

Demir Y, Durmaz L, Taslimi P, Gulçin İ. Antidiabetic properties of dietary phenolic compounds: Inhibition effects on α-amylase, aldose reductase, and α-glucosidase. Biotechnol Appl Biochem. 2019; 66(5): 781-6.

Zhu J, Chen C, Zhang B, Huang Q. The inhibitory effects of flavonoids on α-amylase and α-glucosidase. Crit Rev Food Sci Nutr. 2020; 60(4): 695-708.

Iheanacho C, Akubuiro P, Oseghale I, Imieje V, Erharuyi O, Kennedy O. Evaluation of the antioxidant activity of the stem bark extracts of Anacardium occidentale ( Linn ) Anacardiaceae. Trop J Phytochem Pharm Sci 2023; 2(2): 65-9.

Li K, Yao F, Xue Q, Fan H, Yang L, Li X, Sun L, Liu Y. Inhibitory effects against α-glucosidase and α-amylase of the flavonoids-rich extract from Scutellaria baicalensis shoots and interpretation of structure-activity relationship of its eight flavonoids by a refined assign-score method. Chem Cent J. 2018; 12(1): 82.

Egharevba E, Chukwuemeke-Nwani P, Eboh U, Okoye E, Bolanle I, Oseghale I, Imieje V, Erharuyi O, A F. Evaluation of the antioxidant and hypoglycaemic potentials of the leaf extracts of Stachytarphyta jamaicensis (Verbenaceae). Trop J Nat Prod Res. 2019; 3(5): 170-4.