Pigments, Phenolics, Antioxidant Activity, Anti--Amylase, and In Vitro Anti-Inflammatory Properties of Crude Acetonic Extract from Viola dalatensis http://www.doi.org/10.26538/tjnpr/v7i12.34
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Abstract
Viola dalatensis Gagnep. is a member of the Violaceae family. Viola plants are often used in traditional medicine. Limited data regarding chemicals and biological activities of this species are found in the literature. This study aimed to identify pigments and phenolics in the plant's aerial parts. Besides, antioxidant, anti--amylase, and in vitro anti-inflammatory properties of acetonic extract from the plant were assessed. The level of chlorophyll a in the dried V. dalatensis aerial parts was twice as much as that of chlorophyll b. Among the phenolics examined, kaempferol was the most abundant compound, with an average concentration of 16.21 mg/100 g of dry weight. The antioxidant activity of the extract determined by ABTS and DPPH scavenging tests was shown to be weaker than that of ascorbic acid. The in vitro anti-inflammatory efficacy of the extract assessed by its ability to inhibit albumin denaturation (IC50 = 0.17 ± 0.01 mg/mL) was slightly lower than that of diclofenac (IC50 = 0.09 ± 0.00 mg/mL). The anti--amylase activity of the extract (IC50 = 0.80 ± 0.01 mg/mL) was slightly stronger than that of acarbose (IC50 = 0.92 ± 0.01 mg/mL). These results give a better understanding of the phytochemical components of V. dalatensis and its potential health-endorsing activities.
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