Ethnobotanical and Ethnopharmacological Surveys of <i>Cannabis sativa</i> (Beldiya Species) Use in the Provinces of Taounate and Al Hoceïma http://www.doi.org/10.26538/tjnpr/v7i12.23

Main Article Content

Ali Mzali
Adil M. Mahraz
Abdessamad A. Benlabchir
Hanane Benzeid
Abha C. Hassani
Smail Amalich
Anass Doukkali

Abstract

Cannabis sativa (family Cannabaceae) is a plant of Asian origin, widely recognized for its psychoactive and therapeutic properties, which have been harnessed for recreational and medicinal purposes since ancient times. Currently, a number of scientific studies have investigated the medicinal value of Cannabis, while traditional knowledge regarding its therapeutic and cosmetic applications is steadily declining. This research project aimed to collect data on the traditional uses of Cannabis sativa in the provinces of Taounate and Al Hoceïma, located in Northern Morocco, by conducting an ethnobotanical survey. Eighty-five structured questionnaires were distributed to traditional practitioners, peasants, herbalists, and users of the plant over a period of four months. The questionnaires consisted of open-ended and closed-ended questions, covering topics such as the plant parts used, the modes of administration, the indications, the dosage, the frequency of use, and the adverse effects of Cannabis sativa L. Descriptive statistics was used to analyze the data and chi-square tests were used to determine the associations between the variables. The study revealed that Cannabis sativa is commonly utilized as a recreational or medicinal plant for several purposes, including the treatment of physical and mental health conditions, cosmetic application, and even as a building material. Furthermore, the findings indicated that various factors, such as demographic data and patterns of cannabis use, are associated with the specific plant parts used and the modes of administration.

Article Details

How to Cite
Mzali, A., Mahraz, A. M., Benlabchir, A. A., Benzeid, H., Hassani, A. C., Amalich, S., & Doukkali, A. (2023). Ethnobotanical and Ethnopharmacological Surveys of <i>Cannabis sativa</i> (Beldiya Species) Use in the Provinces of Taounate and Al Hoceïma: http://www.doi.org/10.26538/tjnpr/v7i12.23. Tropical Journal of Natural Product Research (TJNPR), 7(12), 5523–5533. Retrieved from https://tjnpr.org/index.php/home/article/view/3189
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Articles
Author Biographies

Ali Mzali, Laboratory of Analytical Chemistry, Faculty of Medicine and Pharmacy, Mohammed V University in Rabat 10100, Morocco

Laboratory of Phytochemistry, National Agency of Medicinal and Aromatic Plants, B.P. 159, Taounate 34000, Morocco

Abdessamad A. Benlabchir, Laboratory of Phytochemistry, National Agency of Medicinal and Aromatic Plants, B.P. 159, Taounate 34000, Morocco

Laboratory of Natural Substances, Pharmacology, Environment, Modeling, Health and Quality of Life, Faculty of Sciences, Sidi Mohamed Ben Abdellah University, Fez 30 000, Morocco

References

Bachir F, Eddouks M, Arahou M, Fekhaoui M. Origin, Early History, Cultivation, and Characteristics of the Traditional Varieties of Moroccan Cannabis sativa L. Cannabis cannabinoid Res. 2022;7(5):603–15.

Kakudidi E, Tugume P, Asiimwe S, Anywar G. Traditional and modern Health uses of Cannabis sativa L. in africa and its phytochemical and pharmacological profile. In: Agrawal DC, Kumar R, Dhanasekaran M (Eds.). Cannabis/Marijuana for Health. Springer; 2022. 189–210p.

Potter G, Bouchard MM, Decorte MT. World wide weed: Global trends in cannabis cultivation and its control. Glob. Crime. 2011;13(1):40-43

Hourfane S, Mechqoq H, Bekkali AY, Rocha JM, El Aouad N. A Comprehensive Review on Cannabis sativa Ethnobotany, Phytochemistry, Molecular Docking and Biological Activities. Plants. 2023;12(6):1245.

Chouvy PA, Afsahi K. Hashish revival in Morocco. Int J Drug Policy. 2014;25(3):416–23.

Ferrara MS. Peak-experience and the entheogenic use of cannabis in world religions. J Psychedelic Stud. 2021;4(3):179–91.

Afsahi K, Darwich S. Hashish in Morocco and Lebanon: A comparative study. Int J Drug Policy. 2016; 31:190–8.

Afsahi K. La construction socio-économique du cannabis au Maroc: le kif comme produit traditionnel, produit manufacturé et produit de contrebande. Tempo Soc. 2017; 29:99–114.

Chouvy PA. Du kif au haschich: évolution de l’industrie du cannabis au Maroc. Bull Assoc Geogr Fr. 2018;95(95–2):308–21.

Officiel B, York N, Vii C, Ii C, Seul D, Majest QN. Culture et production du cannabis. E Bulleti off. 2021; 1442:1172–80.

Wood L. Medical Marijuana Market Size & Share Report, 2030 [Online]. 2022 [cited 2023 Jun 5]. Available from: https://www.grandviewresearch.com/industry-analysis/medical-marijuana-market

APDN Taounate – Portal of the Northern Development Agency [Online]. 2015 [cited 2022 Nov 17]. Available from: http://www.apdn.ma/index.php?option=com_content&view=article&id=120&Itemid=154&lang=fr

APDN Hoceima – Portal of the Northern Development Agency [Online]. 2015 [cited 2022 Oct 7]. Available from: http://www.apdn.ma/index.php?option=com_content&view=article&id=119&Itemid=155&lang=fr

Mahraz AM, Elhachmia C, Rais Z, Taleb M. Medicinal Plants of Moulay Yaâcoub Province in Morocco: An Ethnobotanical and Biodiversity Survey. Trop J Nat Prod Res. 2023;7(8) :3590-3601http://www.doi.org/10.26538/tjnpr/v7i8.3

Shakil SSM, Gowan M, Hughes K, Azam MNK, Ahmed MN. A narrative review of the ethnomedicinal usage of Cannabis sativa L. as traditional phytomedicine by folk medicine practitioners of Bangladesh. J Cannabis Res. 2021;3(1):1–12.

Bouarfa M, Lebtar S, Boukhira S, Bousta D. An ethnobotanical and ethnopharmacological survey of Cannabis sativa of Taounate Region in Northern Morocco. Int J Pharm Sci Rev Res. 2020;64(2):116–22.

Jeffers AM, Glantz S, Byers A, Keyhani S. Sociodemographic characteristics associated with and prevalence and frequency of cannabis use among adults in the US. JAMA Netw open. 2021;4(11): e2136571–e2136571.

Kona S, Rahman A. Inventory of medicinal plants at Mahadebpur upazila of Naogaon district, Bangladesh. Appl Ecol Environ Sci. 2016;4(3):75–83.

Parent N, Ferlatte O, Milloy MJ, Fast D, Knight R. The sexualised use of cannabis among young sexual minority men:“I’m actually enjoying this for the first time.” Cult Health Sex. 2021;23(7):883–98.

Guyonnet E, Stewart KE, Davis JA. Revealing the meaning of cannabis use as an occupation: a scoping review. Subst Abus Res Treat. 2023; 17:11782218221150112.

Smith R, Hall KE, Etkind P, Van Dyke M. Current marijuana use by industry and occupation—Colorado, 2014–2015. Morb Mortal Wkly Rep. 2018;67(14):409.

Macleod J, Oakes R, Copello A, Crome I, Egger M, Hickman M, Oppenkowski T, Stokes-Lampard H, Smith GD. Psychological and social sequelae of cannabis and other illicit drug use by young people: a systematic review of longitudinal, general population studies. Lancet. 2004;363(9421):1579–88.

Fergusson DM, Horwood LJ, Beautrais AL. Cannabis and educational achievement. Addiction. 2003;98(12):1681–92.

Horwood LJ, Fergusson DM, Hayatbakhsh MR, Najman JM, Coffey C, Patton GC, Silins E, Hutchinson DM. Cannabis use and educational achievement: findings from three Australasian cohort studies. Drug Alcohol Depend. 2010;110(3):247–53.

Marie O, Zölitz U. “High” achievers? cannabis access and academic performance. Rev Econ Stud. 2017;84(3):1210–37.

Rhattas M, Douira A, Zidane L. Étude ethnobotanique des plantes médicinales dans le Parc National de Talassemtane (Rif occidental du Maroc). J Appl Biosci. 2016; 97:9187–211.

Jang BJ, Schuler MS, Evans-Polce RJ, Patrick ME. Marital status as a partial mediator of the associations between young adult substance use and subsequent substance use disorder: Application of causal inference methods. J Stud Alcohol Drugs. 2018;79(4):567–77.

Giles Tremlett. Ketama Gold puts Morocco top of Europe’s cannabis league | World news | The Guardian [Online]. 2003 [cited 2023 Jun 5]. Available from: https://www.theguardian.com/world/2003/may/27/gilestremlett

Dolgin E. The bioengineering of cannabis. Nature. 2019;572(7771): S5–S5.

Schemm P, Bellaoualli S. Breaking taboo, Morocco may okay pot in nod to struggling farmers | The Times of Israel [Online]. 2014 [cited 2023 Jun 5]. Available from: https://www.timesofisrael.com/breaking-taboo-morocco-may-okay-pot-in-nod-to-struggling-farmers/

Manthey J. Cannabis use in Europe: Current trends and public health concerns. Int J Drug Policy. 2019; 68:93–6.

Zheng Z, Fiddes K, Yang L. A narrative review on environmental impacts of cannabis cultivation. J Cannabis Res. 2021;3(1):1–10.

Gobira PH, Joca SR, Moreira FA. Roles of cannabinoid CB1 and CB2 receptors in the modulation of psychostimulant responses. Acta Neuropsychiatr. 2022;1–11.

An D, Peigneur S, Hendrickx LA, Tytgat J. Targeting cannabinoid receptors: current status and prospects of natural products. Int J Mol Sci. 2020;21(14):5064.

Gasperi V, Guzzo T, Topai A, Gambacorta N, Ciriaco F, Nicolotti O, Mauro.M. Recent advances on type-2 cannabinoid (CB2) receptor agonists and their therapeutic potential. Curr Med Chem. 2023;30(12):1420–57.

Schmiedhofer P, Vogel FD, Koniuszewski F, Ernst M. Cys-loop receptors on cannabinoids: All high? Front Physiol. 2022; 13:2349.

Ghovanloo MR, Arnold JC, Ruben PC. Cannabinoid interactions with ion channels, receptors, and the bio-membrane. Front Physiol. 2023; 14:1211230.

Lozano I. The therapeutic use of Cannabis sativa L. in Arabic medicine. J Cannabis Ther. 2001;1(1):63–70.

Ribeiro LIG, Ind PW. Effect of cannabis smoking on lung function and respiratory symptoms: a structured literature review. NPJ Prim care Respir Med. 2016;26(1):1–8.

Preteroti M, Wilson ET, Eidelman DH, Baglole CJ. Modulation of pulmonary immune function by inhaled cannabis products and consequences for lung disease. Respir Res. 2023;24(1):1–19.

Kaplan AG. Cannabis and lung health: does the bad outweigh the good? Pulm Ther. 2021;7(2):395–408.

Meah F, Lundholm M, Emanuele N, Amjed H, Poku C, Agrawal L, Emanuele MA. The effects of cannabis and cannabinoids on the endocrine system. Rev Endocr Metab Disord. 2022;23(3):401–20.

Porr CJ, Rios P, Bajaj HS, Egan AM, Huot C, Batten R, Bishop L, Ryan D, Davis E, Darvesh N, Rahman A, Asghari S, Acheampong L, Tricco AC. The effects of recreational cannabis use on glycemic outcomes and self-management behaviours in people with type 1 and type 2 diabetes: a rapid review. Syst Rev. 2020;9(1):1–10.

Muniyappa R, Sable S, Ouwerkerk R, Mari A, Gharib AM, Walter M, Courville A, Hall G, Chen KY, Volkow ND, Kunos G, Huestis MA, Skarulis MC. Metabolic effects of chronic cannabis smoking. Diabetes Care. 2013;36(8):2415–22.

Grimison P, Mersiades A, Kirby A, Lintzeris N, Morton R, Haber P, Olver I, Walsh A , McGregor I, Cheung Y, Tognela A, Hahn C, Briscoe K, Aghmesheh M, Fox P, Abdi E, Clarke S, Della-Fiorentina S, Shannon J, Gedye C, Stockler M. Oral THC: CBD cannabis extract for refractory chemotherapy-induced nausea and vomiting: a randomised, placebo-controlled, phase II crossover trial. Ann Oncol. 2020;31(11):1553–60.

Lindner T, Schmidl D, Peschorn L, Pai V, Popa-Cherecheanu A, Chua J, Schmetterer L, Garhöfer G. Therapeutic Potential of Cannabinoids in Glaucoma. Pharmaceuticals. 2023;16(8):1149.

Passani A, Posarelli C, Sframeli AT, Perciballi L, Pellegrini M, Guidi G, Figus M. Cannabinoids in glaucoma patients: The never-ending story. J Clin Med. 2020;9(12):3978.

Miller S, Daily L, Leishman E, Bradshaw H, Straiker A. Δ9-Tetrahydrocannabinol and cannabidiol differentially regulate intraocular pressure. Invest Ophthalmol Vis Sci. 2018;59(15):5904–11.

Martins AM, Gomes AL, Vilas Boas I, Marto J, Ribeiro HM. Cannabis-based products for the treatment of skin inflammatory diseases: A timely review. Pharmaceuticals. 2022;15(2):210.

Schofs L, Sparo MD, Sanchez Bruni SF. The antimicrobial effect behind Cannabis sativa. Pharmacol Res Perspect. 2021;9(2): e00761.

Saleemi MA, Yahaya N, Zain NNM, Raoov M, Yong YK, Noor NS, Lim V. Antimicrobial and cytotoxic effects of Cannabinoids: An updated review with future perspectives and current challenges. Pharmaceuticals. 2022;15(10):1228.

Kongkadee K, Wisuitiprot W, Ingkaninan K, Waranuch N. Anti-inflammation and gingival wound healing activities of Cannabis sativa L. subsp. sativa (hemp) extract and cannabidiol: An in vitro study. Arch Oral Biol. 2022; 140:105464.

Procaccia S, Lewitus GM, Lipson Feder C, Shapira A, Berman P, Meiri D. Cannabis for medical use: versatile plant rather than a single drug. Front Pharmacol. 2022; 13:894960.

Baratta F, Pignata I, Ravetto Enri L, Brusa P. Cannabis for medical use: Analysis of recent clinical trials in view of current legislation. Front Pharmacol. 2022; 13:888903.

Chauhan A, Verma R, Kumari S, Sharma A, Shandilya P, Li X, Batoo KM, Imran A, Kulshrestha S, Kumar R. Photocatalytic dye degradation and antimicrobial activities of Pure and Ag-doped ZnO using Cannabis sativa leaf extract. Sci Rep. 2020;10(1):7881.

Idu M, Otuya OS, Obarisiagbon P, Gabriel BO. Anti-inflammatory and analgesic properties of Cannabis sativa L. leaf ethanol extract in animal model. Int j min fru med aromat plants.2022; 8 (2): 54- 62

Martell K, Fairchild A, LeGerrier B, Sinha R, Baker S, Liu H, Ghose A, Olivotto IA, Kerba M. Rates of cannabis use in patients with cancer. Curr Oncol. 2018;25(3):219–25.

Meng H, Dai T, Hanlon JG, Downar J, Alibhai SMH, Clarke H. Cannabis and cannabinoids in cancer pain management. Curr Opin Support Palliat Care. 2020;14(2):87–93.

Wang L, Hong PJ, May C, Rehman Y, Oparin Y, Hong CJ, Hong BY, AminiLari M, Gallo L, Kaushal A, Craigie S, Couban RJ, Kum E, Shanthanna H, Price I, Upadhye S, Ware MA , Campbell F, Buchbinder R, Agoritsas T, Busse JW. Medical cannabis or cannabinoids for chronic non-cancer and cancer related pain: a systematic review and meta-analysis of randomised clinical trials. Br Med J. 2021;374.

Jantjies ZE. Investigating the pain-relief effect of Cannabis sativa and its metabolic products in in vitro prostate and lung cancers [Online]. 2022 [cited 2023 Nov 16]. Available from: https://hdl.handle.net/10210/504021

Moltke J, Hindocha C. Reasons for cannabidiol use: a cross-sectional study of CBD users, focusing on self-perceived stress, anxiety, and sleep problems. J cannabis Res. 2021;3(1):1–12.

Kaul M, Zee PC, Sahni AS. Effects of cannabinoids on sleep and their therapeutic potential for sleep disorders. Neurotherapeutics. 2021; 18:217–27.

Kisiolek JN, Flores VA, Ramani A, Butler B, Haughian JM, Stewart LK. Eight Weeks of Daily Cannabidiol Supplementation Improves Sleep Quality and Immune Cell Cytotoxicity. Nutrients. 2023;15(19):4173.

Spinella TC, Stewart SH, Naugler J, Yakovenko I, Barrett SP. Evaluating cannabidiol (CBD) expectancy effects on acute stress and anxiety in healthy adults: a randomized crossover study. Psychopharmacology (Berl.). 2021; 238:1965–77.

Elms L, Shannon S, Hughes S, Lewis N. Cannabidiol in the treatment of post-traumatic stress disorder: a case series. J Altern Complement Med. 2019;25(4):392–7.

Ryz NR, Remillard DJ, Russo EB. Cannabis roots: a traditional therapy with future potential for treating inflammation and pain. Cannabis cannabinoid Res. 2017;2(1):210–6.

Menezes PMN, de Lima Araújo TC, Pereira ECV, Neto JA, Silva DS, Brito MC, Lima KSB, Monte APO, Matos MHT, Lima RS, Araújo Ribeiro LAA, Silva FS. Investigation of antinociceptive, antipyretic, antiasthmatic, and spasmolytic activities of Brazilian Cannabis sativa L. roots in rodents. J Ethnopharmacol. 2021; 278:114259.

El Khomsi M, Dandani Y, Chaachouay N, Hmouni D. Ethnobotanical study of plants used for medicinal, cosmetic, and food purposes in the region of Moulay Yacoub, Northeast of Morocco. J Pharm Pharmacogn Res. 2022; 10:13–29.

Emre G, Dogan A, Haznedaroglu MZ, Senkardes I, Ulger M, Satiroglu A, Emmez BC, TugaY O. An ethnobotanical study of medicinal plants in Mersin (Turkey). Front Pharmacol. 2021; 12:664500.

Akgul A, Akgul A, Senol SG, Yildirim H, Secmen O, Dogan Y. An ethnobotanical study in Midyat (Turkey), a city on the silk road where cultures meet. J Ethnobiol Ethnomed. 2018;14(1):1–18.

Ning K, Hou C, Wei X, Zhou Y, Zhang S, Chen Y, Yu H, Dong L, Chen S. Metabolomics analysis revealed the characteristic metabolites of hemp seeds varieties and metabolites responsible for antioxidant properties. Front Plant Sci. 2022; 13:904163.

Haddou S, Mounime K, Loukili E, Ou-Yahia D, Hbika A, Idrissi MY, Legssyer A, Lgaz H, Asehraou A, Touzani R, Hammouti B, Chahine A. Investigating the Biological Activities of Moroccan Cannabis Sativa L Seed Extracts: Antimicrobial, Anti-inflammatory, and Antioxidant Effects with Molecular Docking Analysis. Moroccan J Chem. 2023;11(04):11–4.

Leonard W, Zhang P, Ying D, Fang Z. Hempseed in food industry: Nutritional value, health benefits, and industrial applications. Compr Rev food Sci food Saf. 2020;19(1):282–308.

Benkirane C, Moumen A Ben, Fauconnier M-L, Belhaj K, Abid M, Caid HS, Elamrani A, Mansouri F. Bioactive compounds from hemp (Cannabis sativa L.) seeds: optimization of phenolic antioxidant extraction using simplex lattice mixture design and HPLC-DAD/ESI-MS 2 analysis. RSC Adv. 2022;12(39):25764–77.

Smeriglio A, Galati EM, Monforte MT, Lanuzza F, D'Angelo V, Circosta C. Polyphenolic Compounds and Antioxidant Activity of Cold-Pressed Seed Oil from Finola Cultivar of Cannabis sativa L. Phytother Res. 2016; 30(8):1298-307.

Rea Martinez J, Montserrat-De La Paz S, De La Puerta R, García-Giménez MD, Fernández-Arche MÁ. Characterization of bioactive compounds in defatted hempseed (: Cannabis sativa L.) by UHPLC-HRMS/MS and anti-inflammatory activity in primary human monocytes. Food Funct. 2020;11(5):4057–66.

Crini G, Lichtfouse E, Chanet G, Morin-Crini N. Applications of hemp in textiles, paper industry, insulation and building materials, horticulture, animal nutrition, food and beverages, nutraceuticals, cosmetics and hygiene, medicine, agrochemistry, energy production and environment: a review. Environ Chem Lett. 2020;18(5):1451–76. Available from: https://doi.org/10.1007/s10311-020-01029-2

Bowyer JL. Industrial Hemp (Cannabis sativa L.) as a Papermaking Raw Material in Minnesota: Technical, Economic, and Environmental Considerations. Minnesota Environ Nat Resour Trust Fund Dep Wood Pap Sci Univ Minnesota [Online]. 2001 [cited 2023 May 6]. Available from: http://hempgate.us/pdf/hemp_papermaking_2001.pdf

Taaifi Y, Belhaj K, Mansouri F, Rbah Y, Melhaoui R, Houmy N, Benmoumen A, Azeroual E, Addi M, Elamrani A , Caid SH. The Effect of Feeding Laying Hens with Nonindustrial Hemp Seed on the Fatty Acid Profile, Cholesterol Level, and Tocopherol Composition of Egg Yolk. Int J Food Sci. 2023; 2023:1360276.

Gakhar N, Goldberg E, Jing M, Gibson R, House JD. Effect of feeding hemp seed and hemp seed oil on laying hen performance and egg yolk fatty acid content: Evidence of their safety and efficacy for laying hen diets. Poult Sci. 2012; 91(3):701-711.

Goldberg E, Ryland D, Gakhar N, House JD, Aliani M. Fatty acid profile and sensory characteristics of table eggs from laying hens fed hempseed and hempseed oil. J Food Sci. 2012 ;77(4):153-60

Šťastník O, Mrkvicová E, Pavlata L. Industrial hemp in animal feed applications. In: Pojić M, Tiwari BK (Eds.). Industrial Hemp Food and Nutraceutical Applications. Academic Press Inc; 2022. 341–65 p.

Naceiri Mrabti H, Bouyahya A, Naceiri Mrabti N, Jaradat N, Doudach L, Faouzi MEA. Ethnobotanical survey of medicinal plants used by traditional healers to treat diabetes in the Taza region of Morocco. J Evid Based Complementary Altern Med. 2021;2021:5515634.

Kachmar MR, Naceiri Mrabti H, Bellahmar M, Ouahbi A, Haloui Z, El Badaoui K, Bouyahya A , Chakir S. Traditional knowledge of medicinal plants used in the Northeastern part of Morocco. J Evid Based Complementary Altern Med. 2021; 2021:6002949.

El Brahimi R, El Barnossi A, Amrani M, Bari A. Ethnobotanical Study and Biodiversity of Medicinal Plants Used in the Province of Taza North-Eastern Morocco. Trop J Nat Prod Res. 2022;6(11):1814-1831. http://www.doi.org/10.26538/tjnpr/v6i11.12

Ndhlovu PT, Asong JA, Omotayo AO, Otang-Mbeng W, Aremu AO. Ethnobotanical survey of medicinal plants used by indigenous knowledge holders to manage healthcare needs in children. PLoS One. 2023;18(3): e0282113.

Balant M, Gras A, Ruz M, Valles J, Vitales D, Garnatje T. Traditional uses of Cannabis: An analysis of the CANNUSE database. J Ethnopharmacol. 2021; 279:114362.

Özenver N. Cannabis in Healthcare: Ethnobotanical and Pharmaceutical Perspectives and Legal Status in Turkey and the Middle East. In: Agrawal DC, Kumar R, Dhanasekaran M (Eds.). Cannabis/Marijuana for Health. Springer Inc; 2022. 131–54 p.

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