Subacute Toxicity Test of Ethanol Extract of Sungkai Leaves (<i>Peronema canescens</i> Jack.) on Renal Histology of Male Wistar Rats
http://www.doi.org/10.26538/tjnpr/v7i12.22
Keywords:
Subacute toxicity, Sungkai, Renal, Histology, Peronema canescens Jack.Abstract
The Sungkai plant (Peronema canescens Jack) is widely used as an immunostimulant. This study determined the toxicity of the ethanol extract of Sungkai leaves in Wistar rats by histological evaluation of their kidneys. The study used 36 male rats administered ethanol extract of Sungkai leaves for 7, 14 and 21 days at three doses of 140, 280 and 560 mg/kg bw, respectively. In each group, three rats were taken to be sacrificed on days 8, 15 and 22, and their kidneys were exercised for further histological evaluation. The degree of kidney tissue damage and the ratio of kidney organs were noted. The descriptive results showed that the highest kidney damage was due to an extract dose of 560 mg/kg bw with a score of 2.2 in the mild damage category, which occurred at 21 days of administration. The observation of the ratio of kidney organs showed that there was a significant effect of variations in dose and duration of administration on the ratio of kidney organs (p<0.05). However, there was no significant effect on the interaction between dose and duration of administration on the ratio of kidney organs (p>0.05).
References
Yassir M, Asnah A. Pemanfaatan Jenis Tumbuhan Obat Tradisional Di Desa Batu Hamparan Kabupaten Aceh Tenggara. Biot J Ilm Biol Teknol Dan Kependidikan. 2019;6(1):17.
Yulianto S. Penggunaan Tanaman Herbal Untuk Kesehatan. J Kebidanan Dan Kesehat Tradis. 2017;2(1):1–7.
Kurniawidjaja LM, Lestari F, Tejamaya M, Ramdhan Dh. Konsep Dasar Toksikologi Industri. Fkm UI. 2021. 54–73 P.
Adinata M, Sudira Iw, Berata Ik. Efek Ekstrak Daun Ashitaba (Angelica keiskei) Terhadap Gambaran Histopatologi Ginjal Mencit (Mus musculus) Jantan. Bul Vet Udayana. 2012;4(2):55–62.
Dillasamola D, Aldi Y, Wahyuni FS, Rita RS, Dachriyanus, Umar S, Rivai H. Study of Sungkai (Peronema canescens, Jack) leaf extract activity as an immunostimulators with in vivo and in vitro methods. Pharmacogn J. 2021.13(6), 1397–1407
BPOM. Peraturan Badan Pengawas Obat Dan Makanan Nomor 10 Tahun 2022 Tentang Pedoman Uji Toksisitas Praklinik Secara In Vivo. Badan Pengawas Obat dan Makanan Republik Indones. 2022;1–220.
Latief M, Tarigan II, Sari Pm, Aurora Fe. Aktivitas Antihiperurisemia Ekstrak Etanol Daun Sungkai (Peronema canescens Jack) Pada Mencit Putih Jantan. Pharmacon J Farm Indones. 2021;18(1):23–37.
Fransisca D, Kahanjak Dn, Frethernety A. Uji Aktivitas Antibakteri Ekstrak Etanol Daun Sungkai (Peronema canescens Jack) Terhadap Pertumbuhan Escherichia coli Dengan Metode Difusi Cakram Kirby-Bauer. J Pengelolaan Lingkung Berkelanjutan J Environ Sustain Manag. 2020;4(1):460–70.
Departemen Kesehatan Republik Indonesia. Farmakope Herbal Indonesia. Edisi 2. Jakarta: Depkes RI; 2017.
Nirogi R, Goyal VK, Jana S, Pandey SK, Gothi A. What Suits Best For Organ Weight Analysis: Review Of Relationship Between Organ Weight And Body / Brain Weight For Rodent Toxicity Studies. Int J Pharm Sci Res. 2014;5(4):1525-32.
Musyarifah Z, Agus S. Proses Fiksasi Pada Pemeriksaan Histopatologik. J Kesehat Andalas. 2018;7(3):443.
Sugiharto. Mikroteknik. Bogor: Departemen Pendidikan Dan Kebudayaan Direktorat Jendral Pendidikan Tinggi Pusat Antar Universitas Ilmu Hayati; 1989.
Devi V. Basic Histology: A Color Atlas And Text. Basic Histology: A Color Atlas And Text. 2016.
Novia GT. Histopatologi Ginjal Tikus Putih (Rattus norvegicus) Setelah Implan Wire Material Logam Histopathology Of Rat’s (Rattus norvegicus) Kidney After Metal Material Wire Implantation. J Ilm Mhs Vet Fak Kedokt Hewan Univ Syiah Kuala. 2020;4(September):101.
Ayun AQ, Faridah DN, Yuliana ND, Andriyanto A. Pengujian Toksisitas Akut LD50 Infusa Benalu Teh (Scurrula Sp.) Dengan Menggunakan Mencit (Mus musculus). Acta Vet Indones. 2021;9(1):53–63.
Warditiani KN, Sari PS, Yustiantara P, Wirasuta IM. Acute and Sub-Acute Oral Toxicity Profile of Purified Tomato Extract on The Liver And Kidney Functions of Male wistar Rats. Trop J Nat Prod Res. 2021;5(11):1962-5.
Bancroft JD, Gamble M. Theory And Practice Of Histological Techniques. Theory Pract Histol Tech. 2008;3(10).
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