Subacute Toxicity Test of Ethanol Extract of Sungkai Leaves (<i>Peronema canescens</i> Jack.) on Renal Histology of Male Wistar Rats http://www.doi.org/10.26538/tjnpr/v7i12.22
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Abstract
The Sungkai plant (Peronema canescens Jack) is widely used as an immunostimulant. This study determined the toxicity of the ethanol extract of Sungkai leaves in Wistar rats by histological evaluation of their kidneys. The study used 36 male rats administered ethanol extract of Sungkai leaves for 7, 14 and 21 days at three doses of 140, 280 and 560 mg/kg bw, respectively. In each group, three rats were taken to be sacrificed on days 8, 15 and 22, and their kidneys were exercised for further histological evaluation. The degree of kidney tissue damage and the ratio of kidney organs were noted. The descriptive results showed that the highest kidney damage was due to an extract dose of 560 mg/kg bw with a score of 2.2 in the mild damage category, which occurred at 21 days of administration. The observation of the ratio of kidney organs showed that there was a significant effect of variations in dose and duration of administration on the ratio of kidney organs (p<0.05). However, there was no significant effect on the interaction between dose and duration of administration on the ratio of kidney organs (p>0.05).
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