The Effect of Surfactant Combination on the Characteristics, Stability, Irritability, and Effectivity of Astaxanthin Nanoemulsion as Anti-Ageing Cosmetics http://www.doi.org/10.26538/tjnpr/v7i12.21
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Abstract
Astaxanthin is a xanthophyll carotenoid with antioxidant activity. The topical application of astaxanthin can enhance collagen density. However, its penetration into the skin is limited. Therefore, a delivery system, such as nanoemulsion, is needed. One factor influencing nanoemulsion formation is the selection of the type and concentration of surfactant. This study aims to determine the influence of surfactant combinations: Tween 80-Span 20 (F1), Tween 80-Span 60 (F2), and Tween 80-Span 80 (F3) in HLB system 14 on the characteristics, stability, irritability, and effectivity of astaxanthin nanoemulsion as an anti-ageing cosmetic. The nanoemulsion was prepared using the phase inversion composition (PIC) method. The pH values for all three formulas were within the range of normal skin pH (4-6), viscosity followed the order F1<F3<F2, droplet size F1<F3<F2, PDI for all three formulas was below 0.2, turbidity F3<F2<F1, %transmittance F3>F2>F1, interfacial tension F1<F3<F2, and zeta potential for all three formulas fell within the range of ±30 mV. Real-time tests showed that F3 was the most stable formula. All three formulas remained stable after centrifugation, did not cause irritation, and were able to enhance collagen density and skin elasticity in the order of F3>F1>F2. The characteristics of all three formulas meet the criteria for nanoemulsions with droplet sizes below 50 nm and polydispersity index (PDI) below 0.2 and without skin irritation. The Astaxanthin nanoemulsion with Tween 80-Span 80 (F3) surfactant combination was the most stable with better effects. Therefore, it has the potential for further formulation into anti-ageing cosmetic preparations.
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References
Chou H, Lee C, Pan J, Wen Z, Huang S. Enriched Astaxanthin Extract from Haematococcus pluvialis Augments Growth Factor Secretions to Increase Cell Proliferation and Induces MMP1 Degradation to Enhance Collagen Production in Human Dermal Fibroblasts. Int J Mol Sci 2016;17:1-13.
Lima SGM, Freire MCLC, Oliveira VdaS, Solisio C, Converti A, de Lima ÁAN. Astaxanthin delivery systems for skin application: A review. Mar Drugs 2021;19:1-17.
Davinelli S, Nielsen ME, Scapagnini G. Astaxanthin in skin health, repair, and disease: A comprehensive review. Nutrients 2018;10:1-12.
Yoshihisa Y, Rehman Mur, Shimizu T. Astaxanthin, a xanthophyll carotenoid, inhibits ultraviolet-induced apoptosis in keratinocytes. Exp Dermatol 2014;23:178–83.
Naguib YMA. Antioxidant Activities of Astaxanthin and Related Carotenoids. J. Agric. Food Chem 2000;48:1150-1154.
National Library of Medicine – Astaxanthin. Available at: https://pubchem.ncbi.nlm.nih.gov/compound/Astaxanthin. Accessed 21 February 2023.
Kim DM, Hyun SS, Yun P, Lee CH, Byun SY. Identification of an emulsifier and conditions for preparing stable nanoemulsions containing the antioxidant astaxanthin. Int J Cosmet Sci 2012;34:64–73.
Gajendiran A, Abraham J. Nanoemulsions for Drug Delivery. In: Keservani RK, Sharma AK, editors. Nanodispersions for Drug Delivery. New Jersey: Apple Academic Press; 2019.
Tayeb HH, Felimban R, Almaghrabi S, Hasaballah N. Nanoemulsions: Formulation, characterisation, biological fate, and potential role against COVID-19 and other viral outbreaks. Colloids Interface Sci Commun 2021;45:1-19.
Azeem A, Rizwan M, Ahmad FJ, Iqbal Z, Khar RK, Aqil M, Talegaonkar S. Brief / Technical Note Nanoemulsion Components Screening and Selection : a Technical Note. AAPS PharmSciTech 2009;10:69-76.
Erawati, T. Para Methoxycinnamic Acid (PMCA) in Nanoemulsion Drug Delivery Systems Using Soybean Oil, Corn Oil, and Virgin Coconut Oil (VCO) [Dissertation]. Surabaya: Universitas Airlangga; 2015.
Deapsari F, Erawati TM, Soeratri W. Penetration of Ubiquinone (Q10) Nanoemulsion Using Olive Oil Through Rat Penetration of Ubiquinone (Q10) Nanoemulsion Using Olive Oil Through Rat Skin. Int J Pharm Clin Res 2017;9:169-72.
Mayangsari FD, Erawati T, Soeratri W, Rosita N. Characteristics and Physical Stability of NLC-Coenzyme Q10 in Sleeping Mask with Patchouli Oil. J. Farm. Dan Ilmu Kefarmasian Indones 2021;8(2):178-186.
Navarro-Pérez YM, Cedeño-Linares E, Norman-Montenegro O, Ruz-Sanjuan V, Mondeja-Rivera Y, Hernández-Monzón AM., Gonzáles-Bedia, MM. Prediction of the physical stability and quality of O/W cosmetic emulsions using full factorial design. J Pharm Pharmacogn Res 2021;9:98–112.
US Pharmacopeia. The United States Pharmacopeia, USP 39/The National Formulary, NF 34. Rockville, MD: US Pharmacopeia Convention; 2016.
Cho YH, Kim S, Bae EK, Mok CK, Park J. Formulation of a cosurfactant-free O/W microemulsion using nonionic surfactant mixtures. J. Food Sci 2008;73:115–21.
Ernoviya, Afriadi. Formulation and In-Vitro Penetration Test of Ketoconazole Nanoemulsion. Indones. J. Pharm. Clin. Res 2020;3(1):1–7.
Ullah N, Amin A, Alamoudi Rana A, Rasheed SA, Alamoudi Ruaa A, Nawaz A, Raza, M, Nawaz T, Ishtiaq S, Abbas SS. Fabrication and Optimisation of Essential-Oil-Loaded Nanoemulsion Using Box–Behnken Design against Staphylococcus aureus and Staphylococcus epidermidis Isolated from Oral Cavity. Pharmaceutics 2022;14(1640):1-
Cazedey ECL, Carvalho FC, Fiorentino FAM, Gremião MPD, Salgado HRN. Corrositex®, BCOP and HET-CAM as alternative methods to animal experimentation. Brazilian J. Pharm. Sci 2009;45:759–766.
ICCVAM. Recommended Test Method Protocol: Hen’s Egg Test–Chorioallantoic Membrane (HET-CAM) Test Method. ICCVAM Test Method Eval. Rep 2010;13:B30–B38.
Ayuningtyas IN, Mun’im A, Sutriyo. The Study of Safety and Skin Whitening Efficacy of Melinjo (Gnetum gnemon L.) Seed Extract‑Loaded Lipid Particle Gel. Pharmacog Res. 2018;10(4):432–436.
Bhardwaj V, Namkoong J, Tartar O, Diaz I, Mao J, Wu J. In Vitro and Ex Vivo Mechanistic Understanding and Clinical Evidence of a Novel Anti-Wrinkle Technology in Single-Arm, Monocentric, Open-Label Observational Studies. Cosmetics 2022;9(80);1-15.
Plyduang T, Atipairin A, Yoon AS, Sermkaew N, Sakdiset P, Sawatdee S. Formula Development of Red Palm (Elaeis guineensis) Fruit Extract Loaded with Solid Lipid Nanoparticles Containing Creams and Its Anti-Aging Efficacy in Healthy Volunteers. Cosmetics 2022;9(3):1-24.
Etikan I, Musa SA, Alkassim RS. Comparison of Convenience Sampling and Purposive Sampling. Am. J. Theor. Appl. Stat 2016;5(1):1-4
Dasgupta N, Ranjan S. Food Nanoemulsions: Stability, Benefits and Applications. Environmental Chemistry for a Sustainable World. Springer, 2018, 19–48
Gurpreet K, Singh SK. Review of nanoemulsion formulation and characterisation techniques. Indian J Pharm Sci 2018;80:781–89.
Ali SM, Yosipovitch G. Skin pH: From basic science to basic skin care. Acta Derm Venereol 2013;93:261–67.
Márquez AL, Palazolo GG, Wagner JR. Water in oil (w/o) and double (w/o/w) emulsions prepared with spans: Microstructure, stability, and rheology. Colloid Polym Sci 2007;285:1119–28.
Peltonen, L., Hirvonen, J., Yliruusi, J. The effect of temperature on sorbitan surfactant monolayers. J. Colloid Interface Sci 2001;239:134–138.
Ahmad J, Nollet Leo ML. Fundamental Aspects of Food-Grade Nanoemulsions. In: Ahmad J, Nollet LML, editors. Nanoemulsion in Food Technology: Development, Characterisation, and Applications. Boca Raton: CRC Press; 2022.
Czamara K, Adamczyk A, Stojak M, Radwan B, Baranska M. Astaxanthin as a new Raman probe for biosensing of specific subcellular lipidic structures: can we detect lipids in cells under resonance conditions? Cell. Mol. Life Sci 2021;78:3477–3484.
Zhang Z, McClements DJ. Overview of Nanoemulsion Properties: Stability, Rheology, and Appearance. In: Jafari SM, McClements DJ, editors. Nanoemulsions: Formulation, Applications, and Characterisation. United States: Elsevier; 2018.
Tadros T, Izquierdo P, Esquena J, Solans C. Formation and stability of nano-emulsions. Adv Colloid Interface Sci 2004;108–109:303–18.
Elmataeeshy ME, Sokar MS, Bahey-El-Din M, Shaker DS. Enhanced transdermal permeability of Terbinafine through novel nanoemulgel formulation; Development, in vitro and in vivo characterisation. Futur. J. Pharm. Sci 2018;4:18–28.
Bajaj S, Singla D, Sakhuja N. Stability Testing of Pharmaceutical Products. J Appl Pharm Sci 2012;2:129–138.
McClements DJ, Jafari SM. General Aspects of Nanoemulsions and Their Formulation, in: Jafari SM, McClements DJ, editors. Nanoemulsions: Formulation, Applications, and Characterisation. United States: Elsevier; 2018.
Kim C. Advanced Pharmaceutics: Physicochemical Principles. United States: CRC Press; 2004.
Takamura A, Minowa T, Noro S, Tadamichi Kubo T. Effects of Tween and Span Group Emulsifiers on the Stability of o/w Emulsions. Chem Pharm Bull 1979;27:2921–26.
Dhruv D. The Study of Sodium Lauryl Sulfate (SLS) Toxicity. J. Clin. Toxicol 2023;13(4):1–5.
Prebeg T, Omerčić D, Erceg V, Matijašić G. Comparison of Sodium Lauryl Sulfate and Sodium Lauryl Ether Sulfate Detergents for Decellularization of Porcine Liver for Tissue Engineering Applications. Chem. Eng. Trans 2023;100:745–750.
Wilhelm KP, Surber C, Maibach HI. Effect of sodium lauryl sulfate-induced skin irritation on in vitro percutaneous absorption of four drugs. J. Invest. Dermatol 1991;96:963–967.
Pezron I. Skin–Surfactant Interactions. Encycl. Surf. Colloid Sci. Third Ed. 2015:6645–6657.
Yuan CL, Xu ZZ, Fan MX, Liu HY, Xie YH, Zhu T. Study on characteristics and harm of surfactants. J. Chem. Pharm. Res 2014;6(7):2233–2237.
Cortex Technology. DermaLab Combo Instruction Manual. Denmark: Cortex Technology; 2021.
Gniadecka M, Gniadecki R, Serup J, Sondergaard J. Ultrasound structure and digital image analysis of the subepidermal low echogenic band in aged human skin: Diurnal changes and interindividual variability. J. Invest. Dermatol 1994;102(3):362–365.
Abdul Jabbar O, Kashmoola MA, Al-Ahmad BEM, Mokhtar KI, Muhammad N, Abdul Rahim R, Qouta LA. The effect of flaxseed extract on skin elasticity of the healing wound in rabbits. IIUM Med. J. Malaysia 2019;18(1):5–12.
Sharma B, Iqbal B, Kumar S, Ali J, Baboota S. Resveratrol-loaded nanoemulsion gel system to ameliorate UV-induced oxidative skin damage: from in vitro to in vivo investigation of antioxidant activity enhancement. Arch. Dermatol. Res 2019;311:773–793.
Annisa R, Mutiah R, Hakim A, Rahmaniyah DNK. Formulation design and evaluation of hydrocortisone-loaded nanoemulsion and nanoemulsion gel for topical delivery. AIP Conf. Proc 2019;2120.
Abdellatif AA, Abou-Taleb HA. Optimization of nano-emulsion formulations for certain emollient effect. World J. Pharm. Pharm. Sci 2015;4(12):1314–1328.
Ramadon D, McCrudden MTC, Courtenay AJ, Donnelly RF. Enhancement strategies for transdermal drug delivery systems: current trends and applications, Drug Delivery and Translational Research. United States: Springer; 2022.
Dragicevic N, Maibach HI. Percutaneous penetration enhancers chemical methods in penetration enhancement: Modification of the stratum corneum, in: Dragicevic, N., Maibach, H.I. (Eds.), Percutaneous Penetration Enhancers Chemical Methods in Penetration Enhancement: Modification of the Stratum Corneum. Germany: Springer; 2015.
Touitou E, Godin B, Karl Y, Bujanover S, Becker Y. Oleic acid, a skin penetration enhancer, affects Langerhans cells and corneocytes. J. Control. Release 2002;80:1–7.