The Potential of UV-B Irradiated Oyster Mushroom (Pleurotus ostreatus) Powder on Plasma Glucose and TNF-α Levels in Type 2 Diabetic Rats http://www.doi.org/10.26538/tjnpr/v7i12.7
Article Sidebar
Main Article Content
Abstract
The study aimed to investigate the impact of UV-B irradiated oyster mushroom powder (referred to as Oyster-D) on blood sugar and TNF-α levels in type 2 diabetic rats induced with streptozotocin (STZ) over 28 days. Twenty-five male rats were divided into five groups: (A) negative control with STZ induction, (B), (C), (D) treated with Oyster-D at doses of 2000 IU, 4000 IU, 8000 IU, and (E) a normal group without STZ induction.
Following treatment, blood samples were collected to analyze fasting plasma glucose (FPG) levels using the GOD-PAP method, while TNF-α levels were assessed using the ELISA method. Statistical analysis included One-Way ANOVA and Post Hoc LSD for mean FPG level differences and Kruskal Wallis and Mann-Whitney tests for mean TNF-α levels.
The results revealed a significant reduction in mean FPG levels after treatment in the negative control compared to the Oyster-D treatment groups at doses of 2000 IU, 4000 IU, and 8000 IU (p<0.05). Additionally, a notable increase in mean TNF-α levels was observed in the negative control group relative to the Oyster-D treatment group at a dose of 8000 IU (p<0.05).
Overall, Oyster-D exhibited potential in reducing blood sugar levels and elevating TNF-α levels in male Wistar rats induced with STZ, suggesting its possible utility in managing hyperglycemia and TNF-α expression in diabetes.
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
References
Kayaniyil S, Vieth R, Retnakaran R, Knight JA, Qi Y, Gerstein HC, et al. Association of vitamin D with insulin resistance and beta-cell dysfunction in subjects at risk for type 2 diabetes. Diabetes Care. 2010;33(6):1379–81.
Sanda A, Bahrun U, Pakasi RD, Aman AM. Analysis of Vitamin D in Patients with Diabetes Mellitus Type 2. Indones J Clin Pathol Med Lab. 2019;25(2):150.
Saif-Elnasr M, Ibrahim IM, Alkady MM. Role of vitamin D on glycemic control and oxidative stress in type 2 diabetes mellitus. J Res Med Sci. 2017;22(1):1‑5.
Dewi I, Chodidjah C, Atina H. Evaluation of Clitoria ternatea L. Flower Extract in Preventing Complications of Diabetes Mellitus. Trop J Nat Prod Res. 2023;7(10):4908–11.
Hussana A, et al. Reaction Order Analysis fot Changing Provitamin D to Vitamin D2 by Ultraviolet-B (UVB) Radiation in White Oyster Mushrooms (Pleurotus ostreatus). Semarang; 2018.
Jedinak A, Dudhgaonkar S, Wu Q-L, Simon J, Sliva D. Anti-inflammatory activity of edible oyster mushroom is mediated through the inhibition of NF-κB and AP-1 signaling. Nutr J. 2011;10(1):52.
Harinarayan CV. Vitamin D and diabetes mellitus. Hormones. 2014;13(2):163–81.
Sánchez C. Reactive oxygen species and antioxidant properties from mushrooms. Synth Syst Biotechnol. 2017;2(1):13–22.
Purbowati P, Johan A, RMD RK. Pengaruh jamur tiram putih (Pleurotus ostreatus) terhadap kadar glukosa darah, profil lipid dan kadar MDA pada tikus (rattus norvegicus) diabetes melitus. J Gizi Indones. 2016;4(2):131–7.
Malik MA, Jan Y, Al-Keridis LA, Haq A, Ahmad J, Adnan M, et al. Effect of Vitamin-D-Enriched Edible Mushrooms on Vitamin D Status, Bone Health and Expression of CYP2R1, CYP27B1 and VDR Gene in Wistar Rats. J Fungi. 2022;8(8):1–15.
Ruslan K, Reza RA, Damayanti S. Effect of Ultraviolet Radiation On The Formation Of Ergocalciferol (Vitamin D2) In Pleurotus ostreatus. Bionatura. 2011;13(3):255-261.
Anas Y, Rositasati R, Fitriani MR, Suharjono S. Pengembangan Model Hewan Percobaan Tikus Diabetes Mellitus Tipe 2 Karena Resistensi Insulin Yang Diinduksi Dengan Human Insulin Jangka Panjang. J Ilmu Farm dan Farm Klin. 2015;12:16–23.
Wardani IS, Aliza Raudatin Sahly, Indana Eva Ajmala, Dewi Suryani. Vitamin D and Type 2 Diabetes Mellitus: Role in Insulin Resistance, Glycemic Control and Long Term Complications. Unram Med J. 2022;11(2):942–5.
Bhat MH, Mohd M, Dar IH, Bhat JA. Role of vitamin D deficiency in type 2 diabetes: Association or coincidence? Clin Diabetol. 2021;10(2):188–94.
J. G, A. K, Szabó A. Increasing the vitamin D level of oyster mushrooms by UV light. Int J Hortic Sci. 2011;17.
Keflie TS, Nölle N, Lambert C, Nohr D, Biesalski HK. Impact of the natural resource of UVB on the content of vitamin D2 in oyster mushroom (Pleurotus ostreatus) under subtropical settings. Saudi J Biol Sci. 2019;26(7):1724–30.
Jasinghe VJ, Perera CO. Ultraviolet irradiation: The generator of Vitamin D2 in edible mushrooms. Food Chem. 2006;95(4):638–43.
Banlangsawan N, Sanoamuang N. Effect of UV-B irradiation on contents of ergosterol, vitamin D2, vitamin B1 and vitamin B2 in Thai edible mushrooms. Chiang Mai J Sci. 2016;43(1):45–53.
Karadeniz M, Ünal S, Bakır T, Sancı RS. Examination of Vitamin D2 Change of Agaricus bisporus and Pleurotus ostreatus Under The (UV) B Radiaton. Int J Res Sci Manag. 2019;6(1):13–7.
Eleazu CO, Eleazu KC, Chukwuma S, Essien UN. Review of the mechanism of cell death resulting from streptozotocin challenge in experimental animals, its practical use and potential risk to humans. J Diabetes Metab Disord. 2013;12(1):60.
Kardina RN, Nuriannisa F, Andini A, Anita A. Hubungan Asupan Bahan Makanan Sumber Vitamin D Dan Vitamin C Terhadap Penurunan Glukosa Darah Pada Responden Terindikasi Diabetes Mellitus Tipe II. J Islam Med. 2021;5(1):20–6.
Lee CJ, Iyer G, Liu Y, Kalyani RR, Bamba N, Ligon CB, et al. The effect of vitamin D supplementation on glucose metabolism in type 2 diabetes mellitus: A systematic review and meta-analysis of intervention studies. J Diabetes Complications. 2017;31(7):1115–26.
Jayasuriya WJABN, Wanigatunge CA, Fernando GH, Abeytunga DTU, Suresh TS. Hypoglycaemic Activity of Culinary Pleurotus ostreatus and P. cystidiosus Mushrooms in Healthy Volunteers and Type 2 Diabetic Patients on Diet Control and the Possible Mechanisms of Action. Phyther Res [Internet]. 2015;29(2):303–9.
Nweze CC, Ubhenin AE, Lay TU, Muhammad AU. Hypoglycemic, hepatoprotective and hypolipidemic effects of Pleurotus ostreatus in alloxan-induced hyperglycemic rats. Trop J Nat Prod Res. 2017;1(4):163–7.
Schlumberger S, Kristan KČ, Ota K, Frangež R, Molgό J, Sepčić K, et al. Permeability characteristics of cell-membrane pores induced by ostreolysin A/pleurotolysin B, binary pore-forming proteins from the oyster mushroom. FEBS Lett. 2014;588(1):35–40.
Frangež R, Šuput D, Molgó J, Benoit E. Ostreolysin A/pleurotolysin B and equinatoxins: Structure, function and pathophysiological effects of these pore-forming proteins. Toxins. 2017;9(4):1–10.
Gaschler MM, Stockwell BR. Lipid peroxidation in cell death. Biochem Biophys Res Commun. 2017;482(3):419–25.
Yuniarti E. Perbedaan Kadar Tumor Necrosis Factor - Alfa Antara Diabetes Mellitus Tipe 2 Terkontrol Dengan Tidak Terkontrol. Bioscience. 2017;1:18.