The Use of ISSR Marker for Analyzing the Genetic Diversity of Nigerian Date Palms (Phoenix dactylifera L.) https://doi.org/10.26538/tjnpr/v1i3.10
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Abstract
Date palm (Phoenix dactylifera L.) is a major fruit crop of arid climate region in countries of the Middle East and North Africa. DNA markers are powerful tools that can provide information on the relatedness among date palms that are difficult to distinguish morphologically. The present study was aimed at the analysis of genetic diversity that exists among and within 10 date palms
((RCP1 (tissue culture palm), Medjoole, R4P2, R5P7, R6P8, R7P6, R8P1, R8P4, R8P6 and R8P13) grown at the Nigerian Institute for Oil palm Research (NIFOR) experimental Substation Dutse, Jigawa State using Inter-Simple Sequence Repeat (ISSR) markers (14A, 44B, HB-08, HB10, HB-12 and HB-14). DNA was extracted from the 10 date palm leaf samples using DNeasy Mini Kit, PCR amplification was done using ISSR markers and amplification products were analysed by electrophoresis in 2 % agarose gel stained with SYBR Green and photographed under UV light. Among the six ISSR markers used to cluster the 10 date palms, 44B, HB-10 and HB-12 gave the highest variation percentage polymorphism. Genetic distance and relationship were estimated among the date palm varieties. The highest similarity coefficient value (0.84) was observed between R5P7 and R8P6 which seems to be the nearest two varieties and can be closely
regrouped. The similarity coefficient value of 0.00 was obtained between RCP1 and Medjoole date palms, indicating that these were among themselves similar but distinct from the other date palms. The result from this work showed evidence of divergence among the date palms grown in Nigeria
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Asemota O, Eke CR, Emoghene BO, Aisueni NO, Adetunji OA, Gidado RM., Solomon BO. Investigation of somatic embryogenesis for in vitro cultures of date palm (Phoenix dactylifera L.). Acta Hort. 2010; 882:225 – 231.
Ahmed TA, Al-Qaradawi AY. Molecular phylogeny of Qatari date palm genotypes using simple sequence repeats markers. Biotechnol. 2008; 8(1): 126 – 131.
Akkak A, Scariota V, TorelloMarinoni D, Boccacci P, Beltramo C, Botta R. Development and evaluation of microsatellite markers in Phoenix dactylifera L. and their transferability to other Phoenix species. Biologia Plantarum. 2009; 53(1): 164-166.
Arabnezhad H, Bahar M, Mohammadi HR, Latifian M. Development, characterization and use of microsatellite markers for germplasm analysis in date palm (Phoenix dactylifera L.). Scientia Horticulturae. 2012; 134: 150-156.
Shinwari S, Mumtaz AS, Rabbani MA, Akbar F, Shinwari ZK. Genetic divergence inTaramira (Eruca sativa L.) Germplasm based on quantitative and qualitative characters. Pak J Bot. 2013; 45(SI): 375-381.
Khanam S, Sham A, Bennetzen JL, Aly MAM. Analysis of molecular marker-based characterization and genetic variation in date palm (Phoenix dactylifera L.). AJCS. 2012; 6(8): 1236-1244.
Srivashtav VS, Solanki VH, Patel HK, Kansara RV. Molecular Characterization of date palm (Phoenix dactylifera) using combined marker analysis grown in Kutch region of India. J Cell Tiss Res. 2014; 14(1): 4083-4088.
Eissa EA, Abdel-Razek AB, El-Sharabasy SF, Rizk RM. Morphological and molecular characterization of soft date palm (Phoenix dactylifera L.) cultivars in Egypt. Egypt J Genet Cytol. 2009; 38: 269 – 284.
Ibrahim IA, Hashem MH, Hemeida AA, Hassan MM, Maksoud AIA. Characterization of genetic diversity of Date palm (Phoenix dactylifera L.) cultivars collected from New valley governorate (El-Kharga and Dakhleh) based on Morphological variability and molecular markers. Life Sci J. 2014; 11 (11):879-889.
Hussein EHA, Adawy SS, Ismai SEM, El-Itriby Arab J BiotechHA. Molecular characterization of some Egyptian date palm germplasm using RAPD and ISSR markers.. 2004; 8(1): 83 –98.
Adawy SS, Hussein EHA, El-Khishin D, Saker MM, Mohamed AA, El-Itriby HA. Genotyping Egyptian date palm cultivars using RAPD, ISSR, AFLP markers and estimation of genetic stability among tissue culture derived plants. Arab J Biotech. 2004; 8(1): 99 – 114.
Moghaieb REA, Abdel-Hadi AA, Ahmed MRA, Hassan AGM. Genetic diversity and sex determination in date palms (Phoenix dactylifera L.) based on DNA markers. Arab J Biotech. 2010; 13(2): 143 – 156.
Abdulla M, Gamal O. Investigation on molecular phylogeny of some date palm (Phoenix dactylifera L.) cultivars by protein, RAPD and ISSR markers in Saudi Arabia. Australian J Crop Sci. 2010; 4(1): 23 – 28.
Karim K, Chokri B, Amel S, Wafa H, Richid H, Nourdine D. Genetic diversity of Tunisian date palm germplasm using ISSR marker. Int J Bot. 2010; 6(2): 182 – 186.
Al-Hadidi SH, Al-Agha HN, Ahmed TA. Genetic diversity of date palm in Qatar. Paper presented at Symposium on the Foundation Annual Research Forum. Proceedings of Energy and Environment, Qatar University, Doha, December 2010.
Zahedi S, Sakka H, Rhouma A, Ahmed AOM, Marrakchi M, Trifi M. Analysis of Tunisian date palm germplasm using simple sequence repeat primers. Agric J Biotech. 2004; 3(4): 215 – 219.
Khierallah HSM, Al-Sammarraie SKI, Mohammed HI. Molecular characterization of some Iraqi Date Palm cultivars using RAPD and ISSR markers. J Asian Sci Res. 2014; 4(9):490-503
Williams JGK, Kubelk AR, Livak KJ, Rafalski JA, Tingey SV. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucl Acid Res. 1990; 18: 6231-6235.
Dice L.R., Measures of the amount of ecologic association between species. Ecol. 945; 26: 297-302.
Emoghene BO, Asemota O, Idu M, Eke CR. Molecular Characterization of some Date Palms in Nigeria using RAPD markers. J Appl Biol Biotech. 2015; 3(05): 026-030.
Hamza H, Benabderrahim MA, Elbekkay M. Investigation of genetic in Tunisian date palm (Phoenix dactylifera L.) cultivars using ISSR marker systems and their relation with fruit Characteristics. Turk J Biol. 2012; 30:449-458.
Ahmed MM, Soliman SS, Elsayed EH. Molecular identification of Some Egyptian Date Palm males and females varieties (Phoenix dactylifera L.) using DNA markers. J ApplSci Res 2006; 2(5); 270-275.
Haider N, Nabulsi I, MirAli N. Phylogenetic relationships among date palm (Phoenix dactylifera L.) cultivars in Syria using RAPD and ISSR markers. J Biol Res. 2012; 1(2): 12-24.
Sedra MH, Lashermes P, Trouslot P, Combes MC, Hamon S. Identification and genetic diversity analysis of date palm (Phoenix dactylifera L.) varieties from Morocco using RAPD markers. Euphytica 1998; 103: 75 – 82.