The Effect of Myrmecodia pendans Ethanol Extract on Myeloperoxidase Level and Histopathological appearance in Dextran Sodium Sulfate-Induced Colitis Rat doi.org/10.26538/tjnpr/v5i11.9
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Abstract
Myrmecodia pendans is a traditional medicinal plant in Merauke district in Indonesia. This study
is aimed at examining the effect of Myrmecodia pendans ethanol extract (MPE) on
myeloperoxidase (MPO) and histopathological appearance of dextran sodium sulfate (DSS) on
the colon of rats. The MPE extract was obtained from maceration. All animals were acclimatized
with normal feed, and were subsequently divided into 4 groups. Group I (N): normal feed; group
II (CC): Carboxy Methyl Cellulose (CMC 1%); group III (MPE100): MPE 100 mg/kg bw; and
group IV (MPE200): 200 mg/kg bw. The animals in groups II, III, and IV were induced with
DSS for 4 days to obtain colitis model in rats before commencingtreatment for 7 days. MPO
levels did not show a significant difference between the treatment groups (p>0.05). MPO levels
in treatment groups III (10.14±3.69 ng/mL) and IV (11.54±4.25 ng/mL) were slightly lower than
that of group II (14.79±2.73 ng/mL), but were still higher compared to that of group I
(9.83±4.04). There was a significant difference in epithelial surface damage and inflammatory
cell infiltration between groups I and II (p<0.01). There was also a significant difference in
inflammatory cell infiltration between treatment group IV and control group II (p<0.05). It can
be concluded that Myrmecodia pendans ethanol extract plays a role in reducing MPO levels and
decreasing colonic epithelium damage as well as inflammatory cell infiltration.
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References
Chou JW, Lai HC, Chang CH, Cheng KS, Feng CL, ChenTW. Epidemiology and Clinical Outcomes of Inflammatory Bowel Disease: a Hospital-Based Study in Central Taiwan. Gastroenterol Res Pract. 2019; 444-448.
Torres J, Mehandru S, Colombel JF, Peyrin B. Crohn’s Disease: The Lancet. 2017; 389 (10080):1741-1755.
Cheng B, Liang X, Wen Y, Li P, Zhang L, Ma M. Integrative Analysis of Transcriptome-wide Association Study Data and Messenger RNA Expression Profiles Identified Candidate Genes and Pathways for Inflammatory Bowel Disease: J Cell Biochem. 2019; 120(9):1483114837.
Ng SC. Advances in IBD Emerging Trends of Inflammatory Bowel Disease in Asia. Lancet. 2016; 12(3):193-196.
Mustika S, Triana N. The Prevalence, Profile and Risk Factor of Patients with Ulcerative Colitis at Dr. Saiful Anwar Malang General Hospital. J Gastroenterol. 2016; 17:16-20.
Attamimi FA, Ruslami R, Maskoen AM. Uji Aktivitas Antibakteri Ekstrak Kasar Umbi Sarang Semut (Myrmecodia pendans) Dibanding dengan Klorheksidin Terhadap Streptococcus sanguinis. Majalah Kedokteran. 2017; 49 (2):94-101.
WidyawatiT, Pase MA, Daulay M, Sumantri IB, Yusoff NA. Evaluation of Myrmecodia pendans Water Extracts on Hematology Profiles, Liver, Kidney Function and Malondialdehyde Level in Healthy Volunteer. Pharmacogn J. 2020; 12(6s):1489-1493
Mardany MP, Chrystomo LY, Karim AK. Skrining Fitokimia dan Uji Aktivitas Sitotoksik Dari Tumbuhan Sarang Semut (Myrmecodia beccarii Hook.f.) Asal Kabupaten Merauke. J Biol Papua. 2016; (1):13-22.
Sudiono J, Hardina M. The Effect of Myrmecodia pendans Ethanol Extract on Inflamed Pulp: Study on Sprague Dawley Rats. Mol Cell Biomed Sci. 2019; 3(2):115.
Chassaing B, Aitken JD, Malleshappa M, Vijay-Kumar M. Dextran Sulfate Sodium (DSS)-Induced Colitis in Mice. Curr Protoc Immunol. 2014; (SUPPL.104):1-14.
Zhang M, Merlin D. Nanoparticle-Based Oral Drug Delivery Systems Targeting The Colon for Treatment of Ulcerative Colitis. Inflamm Bowel Dis. 2018; 24(7):1401-1415.
Dhurhania CE, Novianto A. Uji Kandungan Fenolik Total dan Pengaruhnya Terhadap Aktivitas Antioksidan Dari Berbagai Bentuk Sediaan Sarang Semut (Myrmecodia pendens). J Farm dan Ilmu Kefarmasian Indones. 2019; 5(2):62.
Depkes. Materia Medika Indonesia Jilid VI. Jakarta : Departemen Kesehatan Republik Indonesia.1995. 300-306 p.
Farnsworth NR. Biological and Phytochemical Screening of
Plants. J. Pharm. Sci.1966; 5(3):225-276.
Richardson PM, Harborne JB. Phytochemical Methods. In Brittonia. 1985; 309:99.
World Medical Association Inc. Declaration of Helsinki. Ethical Principles for Medical Research Involving Human Subjects. J Indian Med Assoc. 2009; 107(6):403-405.
Erben U, Loddenkemper C, Doerfel K, Spieckermann S, Haller D, Heimesaat MM, Zeitz M, Siegmund B, Kühl AA. A guide to Histomorphological evaluation of Intestinal Inflammation in Mouse Models. Int J Clin Exp Pathol. 2014; 7(8):4557-76.
Dahlan S. Statistik Untuk Kedokteran dan Kesehatan Seri 1 Edisi 6. Jakarta: Epidemiologi Indonesia. 2014; 6(1):100-234.
Rajendiran V, Natarajan V, Devaraj SN. Anti-Inflammatory Activity of Alpinia officinarum Hance on Rat Colon Inflammation and Tissue Damage in DSS Induced Acute and Chronic Colitis Models. Food Sci Hum Wellness [Internet]. 2018; 7(4):273–81.
Merdana IM, Watiniasih NL, Sudira IW, Arjana AAG, Gunawan IWNF, Sudimartini LM, and Budiasa K. The Effect of Ethanolic Extract of Myrmecodia pendans on Gentamicin Induced Nephrotoxicity in Wistar Rats. International Journal of Veterinary Science. 2021; 10(2):96-101.
Eichele DD, Kharbanda KK. Dextran Sodium Sulfate Colitis Murine Model: An Indispensable Tool for Advancing Our Understanding of Inflammatory Bowel Diseases Pathogenesis. World Journal of Gastroenterology. 2017; 23(33),6016-6029.
Hidajat NN, Mulyadi D, Tandjung FA, Sulaeman Asep. Potensi Fraksinasi Sarang Semut Papua (Myrmecodia pendans) Pada Penurunan TNF-α dan Perbaikan Secara Histopatologi Kartilago Osteoartritis Lutut Kelinci. Majalah Kedokteran Bandung. 2018; 50(3):181-187.