The Effect of Myrmecodia pendans Ethanol Extract on Myeloperoxidase Level and Histopathological appearance in Dextran Sodium Sulfate-Induced Colitis Rat
doi.org/10.26538/tjnpr/v5i11.9
Keywords:
Colitis, Colon histopathology, Myeloperoxidase, Myrmecodia pendans extractAbstract
Myrmecodia pendans is a traditional medicinal plant in Merauke district in Indonesia. This study
is aimed at examining the effect of Myrmecodia pendans ethanol extract (MPE) on
myeloperoxidase (MPO) and histopathological appearance of dextran sodium sulfate (DSS) on
the colon of rats. The MPE extract was obtained from maceration. All animals were acclimatized
with normal feed, and were subsequently divided into 4 groups. Group I (N): normal feed; group
II (CC): Carboxy Methyl Cellulose (CMC 1%); group III (MPE100): MPE 100 mg/kg bw; and
group IV (MPE200): 200 mg/kg bw. The animals in groups II, III, and IV were induced with
DSS for 4 days to obtain colitis model in rats before commencingtreatment for 7 days. MPO
levels did not show a significant difference between the treatment groups (p>0.05). MPO levels
in treatment groups III (10.14±3.69 ng/mL) and IV (11.54±4.25 ng/mL) were slightly lower than
that of group II (14.79±2.73 ng/mL), but were still higher compared to that of group I
(9.83±4.04). There was a significant difference in epithelial surface damage and inflammatory
cell infiltration between groups I and II (p<0.01). There was also a significant difference in
inflammatory cell infiltration between treatment group IV and control group II (p<0.05). It can
be concluded that Myrmecodia pendans ethanol extract plays a role in reducing MPO levels and
decreasing colonic epithelium damage as well as inflammatory cell infiltration.
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