Molecular Cascade of Neolignan as A Natural Anti-Diabetics Agent: A Bioinformatics Approach


  • Yustina S. Hartini Faculty of Pharmacy, Sanata Dharma University, Paingan, Maguwoharjo, Depok, Sleman, Yogyakarta 55282, Indonesia
  • Brigitta A. Maharani Faculty of Pharmacy, Sanata Dharma University, Paingan, Maguwoharjo, Depok, Sleman, Yogyakarta 55282, Indonesia
  • Kadek A. Widyantara Faculty of Pharmacy, Sanata Dharma University, Paingan, Maguwoharjo, Depok, Sleman, Yogyakarta 55282, Indonesia
  • Bakti W. Saputra Faculty of Pharmacy, Sanata Dharma University, Paingan, Maguwoharjo, Depok, Sleman, Yogyakarta 55282, Indonesia
  • Dewi Setyaningsih Faculty of Pharmacy, Sanata Dharma University, Paingan, Maguwoharjo, Depok, Sleman, Yogyakarta 55282, Indonesia
  • Agustina Setiawati Faculty of Pharmacy, Sanata Dharma University, Paingan, Maguwoharjo, Depok, Sleman, Yogyakarta 55282, Indonesia


bioinformatics, diabetes, gene targets, Neolignan


In cases of chronic diabetes, hyperglycemia triggers the activation of various molecular pathways. To control hyperglycemia in diabetic patients, anti-diabetic agents should precisely target these specific molecular pathways or elaborate on protein targets within the molecular cascade. However, it's important to note that anti-diabetic medications often come with significant side effects, including the risk of hypoglycemic coma and potential liver and kidney complications. Therefore, the inclusion of medicinal plants with anti-hypoglycemic properties continues to be crucial for diabetes management. Neolignan, a biosynthesis product of the shikimate pathway in plants, has previously shown in vitro anti-diabetic activity. This study was designed to identify neolignan's multiple molecular targets and its molecular cascade for inhibiting diabetes. The researchers mined online databases for genes related to diabetes and hyperglycemia, as well as genes affected by neolignan. The Venn diagram result of these genes was further utilized to figure out a network of protein-protein interaction and gene clustering. In summary, the study identified proteins targeted by neolignan, which include IGFR-1, EGFR, the inflammatory cytokine TNF-α, the chaperone protein Hsp90, as well as various downstream signaling molecules such as MAPK8, SCR, PI3K, and JAK1. These proteins work in concert during neolignan treatment to support its anti-diabetic activity. This research provides an initial glimpse into the molecular mechanisms of neolignan in diabetes treatment.


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How to Cite

Hartini, Y. S., Maharani, B. A., Widyantara, K. A., Saputra, B. W., Setyaningsih, D., & Setiawati, A. (2023). Molecular Cascade of Neolignan as A Natural Anti-Diabetics Agent: A Bioinformatics Approach: Tropical Journal of Natural Product Research (TJNPR), 7(11), 5188–5194. Retrieved from

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