Anti-Arthritic Activity of Combination of <i>Caesalpinia sappan</i> and <i>Zingiber officinale</p> Extracts in Complete Freund’s Adjuvant- Induced Arthritic in Rats


  • Tukiran Tukiran Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Negeri Surabaya, Surabaya, Indonesia
  • Nadiah A. Salma Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Negeri Surabaya, Surabaya, Indonesia
  • Suyatno Sutoyo Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Negeri Surabaya, Surabaya, Indonesia
  • Fauzia I. Sabila Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Negeri Surabaya, Surabaya, Indonesia


Several plant species, including sappan and red ginger, have been studied to treat inflammation. Each species has been tested for anti-arthritis, but a combination of the two species has never been done. This study was conducted to test the anti-arthritis potential of a combination of ethanol extract of sappan wood and red ginger using a rat model of Complete Freund's Adjuvant-induced rheumatism. The study involved several percentages of inhibition of rat paw edema volume (%), arthritis index, histopathological profile, and haematological evaluation. The test formula prepared included sappan wood single extract (ES), red ginger single extract (ERG), comparison of the combination of two extracts at doses F1 (1 ES: 1 ERG), F2 (2 ES: 1 ERG), and F3 (1 ES: 2 ERG). The results showed that the F2 formula had the best anti-arthritis effect with an inhibition percentage of rat paw edema volume of 86.72% by showing relatively mild inflammatory cell infiltration, the formation of connective tissue through histopathological observations of rat foot tissue. Haematological observations of platelet increase were highest in group F2 which functions to defend the body from toxic substances. There was no significant difference in the rat haematological parameter in each treatment group (p>0.05).


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How to Cite

Tukiran, T., Salma, N. A., Sutoyo, S., & Sabila, F. I. (2023). Anti-Arthritic Activity of Combination of <i>Caesalpinia sappan</i> and <i>Zingiber officinale</p> Extracts in Complete Freund’s Adjuvant- Induced Arthritic in Rats: Tropical Journal of Natural Product Research (TJNPR), 7(11), 5164–5171. Retrieved from