Evaluation of <i>Salvinia molesta</i> D.S.Mitch (Salviniaceae) for Antioxidant and Antibacterial Properties

http://www.doi.org/10.26538/tjnpr/v7i11.13

Authors

  • Nur A. Md Salleh Faculty of Applied Sciences and Technology, Universiti Tun Hussein Onn Malaysia Pagoh, KM1, Jalan Panchor, 84600 Muar, Johor, Malaysia
  • Furzani Pa’ee Faculty of Applied Sciences and Technology, Universiti Tun Hussein Onn Malaysia Pagoh, KM1, Jalan Panchor, 84600 Muar, Johor, Malaysia
  • Nur A. Manan Faculty of Applied Sciences and Technology, Universiti Tun Hussein Onn Malaysia Pagoh, KM1, Jalan Panchor, 84600 Muar, Johor, Malaysia
  • Siti F. Sabran Faculty of Applied Sciences and Technology, Universiti Tun Hussein Onn Malaysia Pagoh, KM1, Jalan Panchor, 84600 Muar, Johor, Malaysia
  • Fazleen I. Abu Bakar Faculty of Applied Sciences and Technology, Universiti Tun Hussein Onn Malaysia Pagoh, KM1, Jalan Panchor, 84600 Muar, Johor, Malaysia
  • Norhayati Muhammad Faculty of Applied Sciences and Technology, Universiti Tun Hussein Onn Malaysia Pagoh, KM1, Jalan Panchor, 84600 Muar, Johor, Malaysia
  • Mohd F. Abu Bakar Faculty of Applied Sciences and Technology, Universiti Tun Hussein Onn Malaysia Pagoh, KM1, Jalan Panchor, 84600 Muar, Johor, Malaysia
  • Hairul A. Sulaiman Pejabat Taman Botani Johor, Jabatan Landskap Negeri Johor, PTD 8744, Mukim 18 Sri Medan, 83400 Sri Medan, Batu Pahat, Johor, Malaysia

Abstract

Salvinia molesta D.S.Mitch, identified as a troublesome invasive aquatic plant in Malaysia and recognized as the world's most invasive alien species in the Global Invasive Species Database  since 2013, underwent a comprehensive study to explore its phytochemical composition, antioxidant potential, and antibacterial properties. The leaves were meticulously extracted using both distilled water and ethanol, and subsequent quantification involved assessing phenolic, flavonoid, tannin, alkaloid, and saponin contents using standard methods. Additionally, the antioxidant activity of the extracts was evaluated through DPPH, ABTS, and FRAP assays. The ethanol extract stood out with notably higher phytochemical content, particularly in tannin (275.98 ± 3.70 mg TAE/g), followed by phenolic, saponin, flavonoid, and alkaloids. Impressively, the ethanol extract exhibited substantial antioxidant capacity, as evidenced by IC50 values of 4.90 ± 0.25 µg/mL (DPPH), 15.22 ± 1.91 µg/mL (ABTS), and a FRAP value of 298.27 ± 4.04 mg TE/g. Moving beyond antioxidant properties, the ethanol extract showcased robust antibacterial activity, notably against Bacillus cereus, followed by Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa in disc-diffusion tests. Further analysis through minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) assays underscored the ethanol extract's capacity to inhibit growth and effectively eliminate all tested bacterial strains. In summary, this research highlights the ethanol extract of Salvinia molesta as a promising source of phytochemicals with substantial antioxidant and antibacterial properties, surpassing the aqueous extract in efficacy.

Author Biographies

Furzani Pa’ee, Faculty of Applied Sciences and Technology, Universiti Tun Hussein Onn Malaysia Pagoh, KM1, Jalan Panchor, 84600 Muar, Johor, Malaysia

Advanced Herbal and Ethnomedical Research (AdHerb) Focus Group, Universiti Tun Hussein Onn Malaysia, KM1, Jln Panchor, 84600 Panchor, Johor, Malaysia

Siti F. Sabran, Faculty of Applied Sciences and Technology, Universiti Tun Hussein Onn Malaysia Pagoh, KM1, Jalan Panchor, 84600 Muar, Johor, Malaysia

Advanced Herbal and Ethnomedical Research (AdHerb) Focus Group, Universiti Tun Hussein Onn Malaysia, KM1, Jln Panchor, 84600 Panchor, Johor, Malaysia

Fazleen I. Abu Bakar, Faculty of Applied Sciences and Technology, Universiti Tun Hussein Onn Malaysia Pagoh, KM1, Jalan Panchor, 84600 Muar, Johor, Malaysia

Advanced Herbal and Ethnomedical Research (AdHerb) Focus Group, Universiti Tun Hussein Onn Malaysia, KM1, Jln Panchor, 84600 Panchor, Johor, Malaysia

Norhayati Muhammad, Faculty of Applied Sciences and Technology, Universiti Tun Hussein Onn Malaysia Pagoh, KM1, Jalan Panchor, 84600 Muar, Johor, Malaysia

Advanced Herbal and Ethnomedical Research (AdHerb) Focus Group, Universiti Tun Hussein Onn Malaysia, KM1, Jln Panchor, 84600 Panchor, Johor, Malaysia

Mohd F. Abu Bakar, Faculty of Applied Sciences and Technology, Universiti Tun Hussein Onn Malaysia Pagoh, KM1, Jalan Panchor, 84600 Muar, Johor, Malaysia

Advanced Herbal and Ethnomedical Research (AdHerb) Focus Group, Universiti Tun Hussein Onn Malaysia, KM1, Jln Panchor, 84600 Panchor, Johor, Malaysia

References

Mukheriee PK, Wahile A. Integrated approaches towards drug development from Ayurveda and other Indian system of medicines. J Ethnopharmacol. 2006; 103(1): 25-35.

Purushoth PT, Panneerselvam P, Suresh R, Clement AW, Balasubramanian S. GC-MS analysis of ethanolic extract of Canthium parviflorum Lamk Leaf. J App Pharmac Sci. 2013; 3(02): 166-168.

Jo I, Fridley JD, Frank DA. Invasive plants accelerate nitrogen cycling: evidence from experimental woody monocultures. J Ecol. 2017; 105(4): 1105-1110.

Mustafa HM, Hayder G. Performance of Pistia stratiotes, Salvinia molesta, and Eichhornia crassipes aquatic plants in the tertiary treatment of domestic wastewater with varying retention times. App Sci. 2020; 10(24): 9105.

Nithya TG, Jayanthi J, Ragunathan MG. Antioxidant activity, total phenol, flavonoid, alkaloid, tannin, and saponin contents of leaf extracts of Salvinia molesta DS Mitchell (1972). Asian J Pharm Clin Res. 2016; 9(1): 200-203.

Nelson LS. Giant and common Salvinia. Biology and control of aquatic plants, 2009; Gainesville: Cover photograph courtesy of SePRO Corporation.

Thomas PA, Room AP. Taxonomy and control of Salvinia molesta. Nature, 1986; 320(6063): 581-584.

Julien MH, Hill MP, Tipping PW. Salvinia molesta DS Mitchell (Salviniaceae). Weed biological control with arthropods in the tropics. Cambridge University Press: Cambridge; 2009. 378-407 p.

Room PM. Ecology of a simple plant-herbivore system. Biological control of Salvinia. Trends Ecol Evol. 1990; 5(3): 74-79.

Forno IW, Harley KLS. The occurrence of Salvinia molesta in Brazil. Aquatic Botany, 1979; 6(2): 185-187.

Fan P, Marston A. How can phytochemists benefit from invasive plants? Nat Prod Commun. 2009. 4(10).

Choudhary MI, Naheed N, Abbaskhan A, Musharraf SG, Siddiqui H. Phenolic and other constituents of freshwater fern Salvinia molesta. Phytochem. 2008; 69(4), 1018-1023.

Nithya TG, Jayanthi J, Raghunathan MG. Phytochemical, Antibacterial and GC MS analysis of a floatingfern Salvinia molesta DS Mitchell (1972). Int J PharmTech Res. 2015; 8(9): 85-90.

Srilaxmi P, Sareddy GR, Kavi PB, Setty OH, Babu PP. Protective efficacy of natansin, a dibenzoyl glycoside from Salvinia natans against CCl4 induced oxidative stress and cellular degeneration in rat liver. BMC Pharmacol. 2010; 10: 13.

Raja KS, Taip FS, Azmi MMZ, Shishir MRI. Effect of pre-treatment and different drying methods on the physicochemical properties of Carica papaya L. leaf powder. J Saudi Soc Agricul Sci. 2019; 18(2): 150-156.

Razak NA, Shaari AR, Jolkili M, Leng LY. Drying curves and colour changes of Cassia alata leaves at different temperatures. In MATEC Web of Conferences, 2016; (Vol. 78, p. 01020). EDP Sciences.

Mostafa AA, Al-Askar AA, Almaary KS, Dawoud TM, Sholkamy EN, Bakri MM. Antimicrobial activity of some plant extracts against bacterial strains causing food poisoning diseases. Saudi J Biolog Sci. 2018; 25(2): 361-366.

Agu KC, Okolie PN. Proximate composition, phytochemical analysis, and in vitro antioxidant potentials of extracts of Annona muricata (Soursop). Food Sci Nutr. 2017; 5(5): 1029-1036.

Bandiola TMB. Extraction and qualitative phytochemical screening of medicinal plants: A brief summary. Intern J Pharma, 2018; 8(1): 137-143.

Slinkard K, Singleton VL. Total phenol analysis: automation and comparison with manual methods. American J Enolog Viticul. 1977; 28(1): 49-55.

El Far MM, Taie HA. Antioxidant activities, total anthocyanins, phenolics and flavonoids contents of some sweetpotato genotypes under stress of different concentrations of sucrose and sorbitol. Australian J Basic Appl Sci. 2009; 3(4): 3609-3616.

Broadhurst R B, Jones WT. Analysis of condensed tannins using acidified vanillin. J Sci Food Agricul. 1978; 29(9): 788-794.

Obadoni BO, Ochuko PO. Phytochemical studies and comparative efficacy of the crude extracts of some haemostatic plants in Edo and Delta States of Nigeria. Glob J Pure Appl Sci. 2002; 8(2): 203-208.

Harborne JB. Phytochemical methods. London Chapman and Hall, 1973.

Ijoma KI, Egbulefu AVI, Odinma SC. The organic extracts from the leaves of Ficus thonningii Blume, Jatropha tanjorensis J. L Ellis and Saroja and Justicia carnea Lindley as potential nutraceutical antioxidants and functional foods. Trends Phytochem Res. 2023; 7(1): 76-85.

Iqbal E, Salim KA, Lim LB. Phytochemical screening, total phenolics and antioxidant activities of bark and leaf extracts of Goniothalamus velutinus (Airy Shaw) from Brunei Darussalam. J King Saud UniSci. 2015; 27(3): 224-32.

Santhosh P, Nithya TG, Lakshmi SG, Marino GL, Balavaishnavi B, Kamaraj M. Assessment of phytochemicals, antioxidant, antibacterial activity, and profiling of functional molecules in a freshwater fern, Salvinia cucullata Roxb. South African J Botany. 2022; 151: 275-83.

Zgoda JR, Porter JR. A convenient microdilution method for screening natural products against bacteria and fungi. Pharmaceu Biology. 2001; 39(3): 221-5.

Kuete V, Ngameni B, Simo CF, Tankeu RK, Ngadjui BT, Meyer JJ, Lall N, Kuiate JR. Antimicrobial activity of the crude extracts and compounds from Ficus chlamydocarpa and Ficus cordata (Moraceae). J Ethnopharmacol. 2008; 120(1): 17-24.

Ajanal M, Gundkalle MB, Nayak SU. Estimation of total alkaloid in Chitrakadivati by UV-Spectrophotometer. Ancient Sci Life, 2012; 31(4): 198.

Mahdi-Pour B, Jothy SL, Latha LY, Chen Y, Sasidharan S. Antioxidant activity of methanol extracts of different parts of Lantana camara. Asian Paci J Trop Biomed. 2012; 2(12): 960-965.

Zulhaimi HI, Rosli IR, Kasim KF, Akmal HM, Nuradibah MA, Sam ST. A comparative study of Averrhoabilimbi extraction method. In AIP Conference Proceedings. 2017; 1(1885).

Hikmawanti NPE, Fatmawati S, Asri AW. The effect of ethanol concentrations as the extraction solvent on antioxidant activity of Katuk (Sauropus androgynus (L.) Merr.) leaves extracts. In IOP Conference Series: Earth and Environmental Science. 2021; 1(755).

Arawande JO, Akinnusotu A, Alademeyin JO. Extractive value and phytochemical screening of ginger (Zingiber officinale) and turmeric (Curcuma longa) using different solvents. Int J Trad Nat Med, 2018; 8(1): 13-22.

Kamaraj M, Dhana RKV, Nithya TG, Danya U. Assessment of antioxidant, antibacterial activity and phytoactive compounds of aqueous extracts of avocado fruit peel from Ethiopia. Int J Peptide Res Therapeut, 2020; 26: 1549-57.

Silva V, Igrejas G, Falco V, Santos TP, Torres C, Oliveira AM, Pereira JE, Amaral JS, Poeta P. Chemical composition, antioxidant and antimicrobial activity of phenolic compounds extracted from wine industry by-products. Food Cont. 2018; 92: 516-22.

Lee JH, Park KH, Lee MH, Kim HT, Seo WD, Kim JY, Baek IY, Jang DS, Ha TJ. Identification, characterisation, and quantification of phenolic compounds in the antioxidant activity-containing fraction from the seeds of Korean perilla (Perilla frutescens) cultivars. Food Chem. 2013; 136(2): 843-52.

Aleksic V, Knezevic P. Antimicrobial and antioxidative activity of extracts and essential oils of Myrtus communis L. Microbio Res. 2014; 169(4): 240-54.

Bravo L. Polyphenols: chemistry, dietary sources, metabolism, and nutritional significance. Nutr Rev. 1998; 56(11): 317-33.

Jasmine R, Daisy P, Selvakumar BN. A Novel Terpenoid from Elephantopus scaber with Antibacterial Activity against Beta lactamase-Producing Clinical Isolates. Res J Microbio. 2007; 2(10): 770-5.

Ekam VS, Ebong PE. Serum protein and enzyme levels in rats following administration of antioxidant vitamins during caffeinated and non-caffeinated paracetamol induced hepatotoxicity. Nigerian J Physiolog Sci. 2007; 22(1-2).

Umesh CV, Jamsheer AM, Prasad MA. The role of flavonoids in drug discovery—review on potential applications. Res J Life Sci Bioinform Pharm Chem Sci. 2018; 4: 70-7.

Haslam E. Natural polyphenols (vegetable tannins) as drugs: possible modes of action. J Nat Products. 1996; 59(2): 205-15.

Khanbabaee K, Van RT. Tannins: classification and definition. Nat Products Reports. 2001; 18(6): 641-9.

Kashiwada Y, Huang L, Kilkuskie RE, Bodner AJ, Lee KH. New hexahydroxydiphenyl derivatives as potent inhibitors of HIV replication in H9 lymphocytes. Bioorganic Med Chem Letters. 1992; 2(3): 235-8.

Oladunmoye MK. Comparative evaluation of antimicrobial activities and phytochemical screening of two varieties of Acalypha wilkesiana. Int J Trop Med. 2006; 1(3): 1348-56.

Prasad RN, Viswanathan S, Devi JR, Nayak V, Swetha VC, Archana BR, Parathasarathy N, Rajkumar J. Preliminary phytochemical screening and antimicrobial activity of Samanea saman. J Med Plants Res. 2008; 2(10): 268-70.

Westendarp H. Effects of tannins in animal nutrition. Deutsche Tierarztliche Wochenschrift, 2006; 113(7): 264-8.

Sodipo OA, Akinniyi JA, Ogunbameru JV. Studies on certain characteristics of extracts of bark of Pausinystalia johimbe and Pausinystalia macroceras (K Schum) Pierre ex Beille. Global J Pure App Sci. 2000; 6(1): 83-8.

Kittakoop P, Mahidol C, Ruchirawat S. Alkaloids as important scaffolds in therapeutic drugs for the treatments of cancer, tuberculosis, and smoking cessation. Curr. Topics in Med. Chem, 2014; 14(2): 239-52.

Cushnie TT, Cushnie B, Lamb AJ. Alkaloids: An overview of their antibacterial, antibiotic-enhancing and antivirulence activities. Int J Antimicrob Agents. 2014; 44(5): 377-86.

Shi Q, Hui S, Zhang A, Hong-Ying X, Guang-Li Y, Ying H, Xi-Jun W. Natural alkaloids: basic aspects, biological roles, and future perspectives. Chinese J Nat Med. 2014; 12(6): 401-6.

Hassan SB, Gullbo J, Hu K, Berenjian S, Morein B, Nygren P. The Nanoparticulate Quillaja Saponin BBE is selectively active towards renal cell carcinoma. Anticancer Res, 2013; 33(1): 143-51.

Sheikh N, Kumar Y, Misra AK, Pfoze L. Phytochemical screening to validate the ethnobotanical importance of root tubers of Dioscorea species of Meghalaya, North East India. J Med Plants. 2013; 1(6): 62-9.

Narayani M, Johnson M, Sivaraman A, Janakiraman N. Phytochemical and antibacterial studies on Jatropha curcas L. J Chemical Pharmaceu Res. 2012; 4(5): 2639-42.

Elumalai A, Eswaraiah MC. A pharmacological review on Garcinia indica Choisy. Int J. Univers Pharm Life Sci. 2011; 1: 508-206.

Li X, Wu X, & Huang L. Correlation between antioxidant activities and phenolic contents of radix Angelicae sinensis (Danggui). Molecules. 2009; 14(12), 5349-5361.

Mardawati E, Filianty F, Marta H. Antioxide activity study of manggis skin extract (Garcinia mangostana L) in order to use manggis skin waste in puspahiang district, tasikmalaya regency. Teknotan: Jurnal Industri & Teknologi Pertanian. 2008; 2(3), 1-5.

Rebaya A, Belghith SI, Baghdikian B, Leddet VM, Mabrouki F, Olivier E, Cherif JK, Ayadi MT. Total phenolic, total flavonoid, tannin content, and antioxidant capacity of Halimium halimifolium (Cistaceae). J Appl Pharm Sci. 2015; 5: 052-057.

Shan B, Cai YZ, Brooks JD, Corke H. The in vitro antibacterial activity of dietary spice and medicinal herb extracts. Int J Food Microbio. 2007; 117(1): 112-9.

Sagbo I, Mbeng W. Plants used for cosmetics in the Eastern Cape Province of South Africa: A case study of skin care. Pharmacog Rev. 2018; 12(24), 139-156.

Mogana R, Adhikari A, Tzar MN, Ramliza R, Wiart C. Antibacterial activities of the extracts, fractions and isolated compounds from Canarium patentinervium Miq. against bacterial clinical isolates. BMC Complement Med Theraphy. 2020; 20(1), 1-11.

Yisa J. Phytochemical analysis and antimicrobial activity of Scoparia dulcis and Nymphaea lotus. Aus J Basic App Sci. 2009; 3(4): 3975-3979.

Omar K, Geronikaki A, Zoumpoulakis P, Camoutsis C, Soković M, Ćirić A, Glamočlija J. Novel 4-thiazolidinone derivatives as potential antifungal and antibacterial drugs. Bioorganic Med Chem. 2010; 18(1): 426-32.

de Almeida CG, Garbois GD, Amaral LM, Diniz CC, Le Hyaric M. Relationship between structure and antibacterial activity of lipophilic N-acyldiamines. Biomed Pharmacotherapy, 2010; 64(4), 287-290.

Published

2023-12-01

How to Cite

Salleh, N. A. M., Pa’ee, F., Manan, N. A., Sabran, S. F., Abu Bakar, F. I., Muhammad, N., … Sulaiman, H. A. (2023). Evaluation of <i>Salvinia molesta</i> D.S.Mitch (Salviniaceae) for Antioxidant and Antibacterial Properties: http://www.doi.org/10.26538/tjnpr/v7i11.13. Tropical Journal of Natural Product Research (TJNPR), 7(11), 5106–5114. Retrieved from https://tjnpr.org/index.php/home/article/view/3010