Effectiveness of Moringa oleifera Nanoparticles (Self Nano Emulsifying Drug Delivery System) on Insulin Resistance in the Prediabetes Rattus norvegicus Model



  • Esri Rusminingsih Medicine and Health Sciences Doctoral Study Program, Faculty of Medicine, Diponegoro University, Semarang, Central Java Indonesia
  • Hardhono Susanto Department of Physiology Anatomy, Faculty of Medicine, Diponegoro University, Semarang, Central Java. Indonesia
  • Diana N. Afifah Department of Nutrition Science, Faculty of Medicine, Diponegoro University, Central Java, Indonesia
  • Ronny Martien Departement of Pharmaceutics, Faculty of Pharmacy, Gadjah Mada University, Yogyakarta, Central Java. Indonesia
  • Hertanto Wahyu Subagyo Departement of Nutrition Consultant, Diponegoro University, Central Java, Indonesia


prediabetes, nanoparticles, Moringa oleifera, insulin resistance, inflammatory cytokines, HOMA-IR


The present study aimed to investigate the effect of Moringa oleifera nanoparticles (MoNP) supplementation based on the Self Nano Emulsifying Drug Delivery System on insulin resistance in prediabetes models. Twenty-five Rattus norvegicus were split into five groups, containing five rats each: normal control group (given a standard diet), as well as a prediabetes control group and three intervention groups fed a high-fat diet for four weeks. Those with fasting blood serum (FBS) ranged betwen 100-130 mg/dL were deemed prediabetic. The intervention groups were then given MoNP at dosages of 75, 125, and 225 mg/kg bw, respectively, whereas the normal and prediabetic groups received a regular diet. FBS, fasting insulin, TNF-α, IL-6, triglycerides, and HOMA-IR were all examined after treatment. MoNP significantly reduced TNF-α, IL-6, triglycerides, and HOMA-IR levels (p<0.05). It also generated a considerable rise in insulin levels in contrast to the prediabetic control group (p<0.05). Supplementation of MoNP at the lowest dose of 75 mg/kg body weight was able to lower FBS to normal levels from 130.04 mg/dL to 97.34 mg/dL and reduce HOMA-IR from 4.20 to 3.71 (p < 0.05). The administration of MoNP can reduce inflammatory cytokines and insulin resistance in the prediabetes model.

Author Biography

Esri Rusminingsih, Medicine and Health Sciences Doctoral Study Program, Faculty of Medicine, Diponegoro University, Semarang, Central Java Indonesia

Nursing Study Program, Faculty of Health and Technology, Universitas Muhammadiyah Klaten, Central Java, Indonesia


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How to Cite

Rusminingsih, E., Susanto, H., Afifah, D. N., Martien, R., & Subagyo, H. W. (2023). Effectiveness of Moringa oleifera Nanoparticles (Self Nano Emulsifying Drug Delivery System) on Insulin Resistance in the Prediabetes Rattus norvegicus Model: http://www.doi.org/10.26538/tjnpr/v7i11.8. Tropical Journal of Natural Product Research (TJNPR), 7(11), 5059–5066. Retrieved from https://tjnpr.org/index.php/home/article/view/3005