Antioxidant Activity of Polysaccharides from Water Lettuce (Pistia stratiotes) Leaf Extract


  • Sabri Sudirman Fisheries Product Technology, Faculty of Agriculture, Universitas Sriwijaya, Indralaya 30862, Ogan Ilir Regency, South Sumatra, Indonesia;
  • Yohana N. Sirait isheries Product Technology, Faculty of Agriculture, Universitas Sriwijaya, Indralaya 30862, Ogan Ilir Regency, South Sumatra, Indonesia;
  • Aatikah D. Ghaisani Fisheries Product Technology, Faculty of Agriculture, Universitas Sriwijaya, Indralaya 30862, Ogan Ilir Regency, South Sumatra, Indonesia;
  • Herpandi Fisheries Product Technology, Faculty of Agriculture, Universitas Sriwijaya, Indralaya 30862, Ogan Ilir Regency, South Sumatra, Indonesia;
  • Indah Widiastuti Fisheries Product Technology, Faculty of Agriculture, Universitas Sriwijaya, Indralaya 30862, Ogan Ilir Regency, South Sumatra, Indonesia;
  • Miftahul Janna Master Program in Agribusiness, Faculty of Agriculture, Universitas Sriwijaya, Palembang City 30139, South Sumatra, Indonesia


polysaccharides, glucose, extraction, defatted, Antioxidant


An increase in free radicals in the body causes oxidative stress. Therefore, our body needs exogenous antioxidants. This study aimed to determine the antioxidant activity of crude and defatted polysaccharide extracts from water lettuce (Pistia stratiotes) leaf. The polysaccharide was extracted by hot-water extraction and defatted with acetone. The total sugar content was determined by the Phenol-Sulfuric acid method, the antioxidant activity by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method, and the functional group was analyzed by Fourier transform infrared (FT-IR) spectroscopy. The results showed that the total sugar of the crude polysaccharide (96.80±14.60 mg glucose eq./g dry sample) had a higher total sugar than the defatted polysaccharide (44.0±2.16 mg glucose eq./g dry sample). However, the antioxidant activity in defatted polysaccharides (IC50 0.406±0.011 mg/mL) more effectively inhibits free radicals than crude extract (IC50 0.484±0.028 mg/mL). The O-H, C-H, C=O, and C-O functional groups were identified in the polysaccharide extracts. The polysaccharides extract from water lettuce exhibits antioxidant activity. Therefore, the polysaccharide from water lettuce (P. stratiotes) leaf can be used as a source of antioxidant agents and as a food supplement.


Meo SD, Venditti P. Evolution of the Knowledge of free radicals and other oxidants. Oxid Med Cell Longev. 2020; 2020:9829176.

Bai L, Xu D, Zhou YM, Zhang YB, Zhang H, Chen YB, Cui YL. Antioxidant activities of natural polysaccharides and their derivatives for biomedical and medicinal applications. Antioxidants. 2022; 11:2491.

Sudirman S, Herpandi, Safitri E, Apriani EF, Taqwa FH. Total polyphenol and flavonoid contents and antioxidant activities of water lettuce (Pistia stratiotes) leave extracts. Food Res. 2022; 6:205-210.

Sudirman S, Janna M, Herpandi, Widiastuti I. In vitro inhibitory HMG-CoA reductase activity of purified polyphenol compounds from water lettuce (Pistia stratiotes) leaf extract. Trop. J. Nat. Prod. Res. 2022; 6:1131-1134.

Šamec D, Karalija E, Šola I, Bok VV, Sondi BS. The role of polyphenols in abiotic stress response: the influence of molecular structure. Plants. 2021; 10:118.

Song YR, Han AR, Park SG, Cho CW, Rhee YK, Hong HD. Effect of enzyme-assisted extraction on the physicochemical properties and bioactive potential of lotus leaf polysaccharides. Int. J. Biol. Macromol. 2020; 153:169-179.

Xu J, Hou H, Hu J, Liu B. Optimized microwave extraction, characterization and antioxidant capacity of biological polysaccharides from Eucommia ulmoides oliver leaf. Sci. Rep. 2018; 8:6561.

Yeh SH, Hsu WK, Chang ZQ, Wang SH, Hsieh CW, Liou GG, Lee HB, Jiang BH, Tsou HK, Tsai MS. Purification and characterization of fractions containing polysaccharides from Talinum triangulare and their immunomodulatory effects. Processes. 2021; 9:709.

Zhang S, Li XZ. Inhibition of α-glucosidase by polysaccharides from the fruit hull of Camellia oleifera Abel. Carbohydr. Polym. 2015; 115:38-43.

Wang B, Liu Q, Huang Y, Yuan Y, Ma Q, Du M, Cai T, Cai Y. Extraction of polysaccharide from Spirulina and evaluation of its activities. Evid Based Complement Alternat Med. 2018; 2018:3425615.

Liu C, Liu Q, Sun J, Jiang B, Yan J. Extraction of water-soluble polysaccharide and the antioxidant activity from semen cassiae. J Food Drug Anal. 2014; 22:492-499.

Kim TK, Yong HI, Kim YB, Jung S, Kim HW, Choi YS. Effects of organic solvent on functional properties of defatted proteins extracted from Protaetia brevitarsis larvae. Food Chem. 2021; 336.

Jeong MS, Lee SD, Cho SJ. Effect of Three defatting solvents on the techno-functional properties of an edible insect (Gryllus bimaculatus) protein concentrate. Molecules. 2021; 26:5307.

Nor NANM, Abbasiliasi S, Marikkar MN, Ariff A, Amid M, Lamasudin DU, Manap MYA, Mustafa S. Defatted coconut residue crude polysaccharides as potential prebiotics: Study of their effects on proliferation and acidifying activity of probiotics in vitro. J. Food Sci. Technol. 2016; 54:164-173.

Kim TK, Lee JH, Yong HI, Kang MC, Cha JY, Chun JY, Choi YS. Effects of defatting methods on the physicochemical properties of proteins extracted from Hermetia illucens larvae. Foods. 2022; 11:1400.

Njanje I, Bagla VP, Beseni BK, Mbazima V, Lebogo KW, Mampuru L, Mokgotho MP. Defatting of acetone leaf extract of Acacia karroo (Hayne) enhances its hypoglycaemic potential. BMC Complement Altern. Med. 2017; 17:482.

Nielsen SS. In Food Analysis Laboratory Manual, 2017, DOI: 10.1007/978-3-319-44127-6_14, ch. Chapter 14, pp. 137-141.

Egharevba E, Nwani PC, Eboh U, Okoye E, Bolanle I, Oseghale I, Imieje V, Erharuyi O, Falodun A. Evaluation of the antioxidant and hypoglycaemic potentials of the leaf extracts of Stachytarphyta jamaicensis (Verbenaceae). Trop. J. Nat. Prod. Res. 2019; 3:170-174.

Bhateja PK, Kajal A, Singh R. Amelioration of diabetes mellitus by modulation of GLP-1 via targeting alpha-glucosidase using Acacia tortilis polysaccharide in streptozotocin-nicotinamide induced diabetes in rats. Journal of Ayurveda and Integrative Medicine. 2020; 11:405-413.

Shofia SI, Jayakumar K, Mukherjee A, Chandrasekaran N. Efficiency of brown seaweed (Sargassum longifolium) polysaccharides encapsulated in nanoemulsion and nanostructured lipid carrier against colon cancer cell lines HCT 116. RSC Adv. 2018; 8:15973-15984.

Amid BT, Mirhosseini H. Effect of Different purification techniques on the characteristics of heteropolysaccharide-protein biopolymer from durian (Durio zibethinus) seed. Molecules. 2012; 17:10875-10892.

Kakar MU, Karim H, Shabir G, Iqbal I, Akram M, Ahmad S, Shafi M, Gul P, Riaz S, Rehman RU, Salari H. A review on extraction, composition, structure, and biological activities of polysaccharides from different parts of Nelumbo nucifera. Food Sci. Nutr. 2023; 00:1-20.

Aldairi AF. Evaluation of various methodologies used in purification of biologically active carbohydrates derived from marine life. Biomed. J. Sci. Technol. Res. 2020; 27:20919- 20927.

Yang L, Liang Q, Wang S, Yuan F, Wang J, Zhang Y, He Y. Quality evaluation of thirteen geographical populations of lycium chinense using quantitative analysis of nutrients and bioactive components, J. Food Qual. 2019; 2019:1-14.

Danilchuk YV. Thermal effect of carbohydrate dissolution in aqueous-organic media. Health Food Biotechnol. 2021; 2:49-59.

Zhu H, Zhang Y, Guo X, Cheng Y, Wang L, Li X. Efficient one-pot production of 5-hydroxymethylfurfural from glucose in an acetone–water solvent. Ind. Eng. Chem. Res. 2022: 61:5661-5671.

Motagamwala AH, Huang K, Maravelias CT, Dumesic JA. Solvent system for effective near-term production of hydroxymethylfurfural (HMF) with potential for long-term process improvement. Energy Environ. Sci. 2019, 12, 2212-2222.

Dhahri M, Sioud S, Alsuhaymi S, Almulhim F, Haneef A, Saoudi A, Jaremko M, Emwas AHM. Extraction, characterization, and antioxidant activity of polysaccharides from ajwa seed and flesh, Separations. 2023; 10:103.

Olugbami JO,. A. Gbadegesin MA, Odunola OA. In vitro evaluation of the antioxidant potential, phenolic and flavonoid contents of the stem bark ethanol extract of Anogeissus leiocarpus. Afr. J. Med. Med. Sci. 2014; 43:101-109.

Matuszewska A, Jaszek M, Stefaniuk D, Ciszewski T, Matuszewski L. Anticancer, antioxidant, and antibacterial activities of low molecular weight bioactive subfractions isolated from cultures of wood degrading fungus Cerrena unicolor. Plos One. 2018; 13:e0197044

Huang H, Huang G. Extraction, separation, modification, structural characterization, and antioxidant activity of plant polysaccharides. Chem Biol Drug Des. 2020; 96:1209-1222.

Fernandes PAR, M. A. Coimbra MA. The antioxidant activity of polysaccharides: A structure-function relationship overview. Carbohydr. Polym. 2023; 314:120965.

Coates J. In Interpretation of Infrared Spectra: A Practical Approach, ed. R. Meyers, John Wiley & Sons Ltd., Chichester. 2000. pp. 10881-10882.

Yang K, Zhang Y, Cai M, Guan R, Neng J, Pi X, Sun P. In vitro prebiotic activities of oligosaccharides from the by-products in Ganoderma lucidum spore polysaccharide extraction. RSC Adv. 2020; 10:14794-14802.



How to Cite

Sudirman, S., Sirait, Y. N., Ghaisani, A. D., Herpandi, Widiastuti, I., & Janna, M. (2023). Antioxidant Activity of Polysaccharides from Water Lettuce (Pistia stratiotes) Leaf Extract: Tropical Journal of Natural Product Research (TJNPR), 7(11), 5055–5058. Retrieved from