Improvement of Insulin Secretion and Pancreatic β-Cell Function in Streptozotocin-induced Diabetic Rats Treated with Dioscorea esculenta Extract: http://www.doi.org/10.26538/tjnpr/v7i11.6

Nilawati Uly; Ari Yuniastuti; Roro Susanti; Yanuarita Tursinawati

Authors

Nilawati Uly Institute of Higher Health Education, Mega Buana Palopo, Indonesia
Ari Yuniastuti Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Semarang, Central Java, Indonesia
Roro Susanti Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Semarang, Central Java, Indonesia
Yanuarita Tursinawati Faculty of Medicine, University of Muhammadiyah Semarang, Central Java, Indonesia

Keywords:

Streptozotocin, Pancreatic β-cells, Inulin, Insulin, Dioscorea esculenta, Diabetes

Abstract

Diabetes mellitus (DM) is a metabolic disorder characterized by chronic hyperglycemia that can destroy pancreatic β-cells due to its toxic effect. A local Indonesian vegetable known as lesser yam (Dioscorea esculenta) possesses anti-diabetic properties. The present study evaluated the effect of inulin extract from lesser yam on pancreatic β-cell count and insulin expression in streptozotocin (STZ)-induced diabetic rats. Inulin was extracted from lesser yams. Streptozotocin was used to induce diabetes in Wistar rats. The rats were divided into six groups: I (control), II (DM), III (galvus drug-control), IV, and V (DM rats administered with inulin at 100 and 300 mg/kg body weight for 21 days, respectively), and VI (DM rats administered with inulin extract at 300 mg/kg body weight for 30 days). Pancreatic β-cell count and insulin expression were assessed using immunohistochemical and histopathological methods, respectively. The results showed that on the 21st and 28th days after treatment, there was a significant difference in the number of pancreatic β-cells between the groups. On day 14, the insulin expression of pancreatic β-cells between groups was significantly different. There was a significant difference in the insulin expression of pancreatic β-cells on the 21st and 28th-day treatments between the various groups. The findings of the study reveal an increase in the β-cell number and insulin expression of pancreatic β-cells in diabetic rats when inulin extract from lesser yam was administered. The inulin dose variation had no significant effect on the number and insulin expression of pancreatic β-cells in Wistar rats.

References

American Diabetes Association (ADA). Standards of Medical Care in Diabetes-2018. Diab Care. 2018; 41(1): S14-S28.

Ministry of Health. The report of Basic Health Research (RISKESDAS). Indon Center Health Res Dev. The National Institute of Health Research and Development. 2013; 145-146.

Meier JJ, Bonadonna RC. Role of reduced β-cell mass versus impaired β-cell function in the pathogenesis of type 2 diabetes. Diab Care. 2013; 2: S113– S119.

Robertson RP, Harmon J, Tran PO, Poitout V. β-cell glucose toxicity, lipotoxicity, and chronic oxidative street in type 2 diabetes. Diab. 2004; 53(1): 119-24.

Cnop M, Welsh N, Jonas JC, Jorns A, Lenzen S, Eizirik DL. Mechanisms of pancreatic beta-cell death in type 1 and type 2 diabetes: many differences, few similarities. Diab. 2005; 54(2): S97–107.

Butler AE, Janson J, Bonner-Weir S, Ritzel R, Rizza RA, Butler PC. Beta-cell deficit and increased beta-cell apoptosis in humans with type 2 diabetes. Diabs. 2003; 52: 102–110.

Shajeela PS, Mohan VR., Jesudas LL, and Soris PT. Nutritional and Antinutritional Evaluation of Wild Yam (Dioscorea spp.) TSA. 2011; 14: 723-730.

AY. Prabowo Karakteristik Fisiko Kimia, Bioaktif, dan Organoleptik Mie Berbasis Tepung Gembili (Dioscorea esculenta L.). Skripsi. Universitas Brawijaya, Malang, Indonesia. 2013; 135.

GM Karunia. Pembuatan Inulin bubuk dari umbi Gembili (Dioscorea esculanta) dengan metode Foam Mat Drying. Skripsi. Food Technology Studies Program. Industrial Technology Faculty UON “Veteran” Jawa Timur, Surabaya, Indonesia. 2013; 58.

Ari Y, Retno SI, Wijayanti N. Pengembangan Pangan Fungsional Berbasis umbi-umbian sebagai sumber antioksidan dalam upaya meningkatkan derajat kesehatan masyarakat melalui pendekatan nutrigenomik. Research Report. Research institutions and community service. Universitas Negeri Semarang. 2015; 46.

Son IS, Kim JH, Sohn HY, Son KH, Kim JS, Kwon CS. Antioxidative and hypolipidemic effects of diosgenin, a steroidal saponin of yam (Dioscorea spp.), on high-cholesterol fed rats. Biosci. Biotechnol Biochem. 2007; 71: 3063–3071.

Kwon JE, Kwon JB, Kwun IS, Sohn HY. Antimicrobial and antioxidant activity of the Dioscorea alata L. J. Microbiol. Biotechnol. 2010; 38: 283–288.

Chang SJ, Lee YC, Liu SY, Chang TW. Chinese yam (Dioscorea alata cv. Tainung No. 2) feeding exhibited antioxidative effects in hyperhomocysteinemia rats. J Agric Food Chem. 2004; 52: 1720–1725.

Go HK, Md.Mahbubur R, Gi BK,Chong SN, Choon HS, Jin SK, Shang JK, Hyung SK. Antidiabetic effects of yam (Dioscorea batatas) and its active constituent, allantoin, in a rat model of streptozotocin-induced diabetes. Nutrients. 2015; 7(10): 8532-8544.

Tursinawati Y, Retno SI, Ari Y. Antidiabetic Activity of inulin from gembili (Dioscorea esculenta) through the inhibition of α glucosidase enzyme in vitro. Proceedings of the 11th International Conference on Preventive Medicine; 2018 May 2-3, Faculty of Medicine Universitas Brawijaya. 2018; 246.

Mudannayake DC, Wimalasiri KMS, Silva KFST, Ajlouni S. Comparison of properties of new sources of partially purified inulin to those of commercially pure chicory inulin. J Food Sci. 2015; 80: C950–960.

Shang HM, Hai ZZ, Jun YY, Ran L, Hui SH XW. In vitro and in vivo antioxidant activities of inulin. PLoS ONE. 2018; 13(2): 1-12.

Winarti, S, Harmayani E, Marsono Y, Pranoto Y. Pengaruh Foaming Pada Pengeringan Inulin Umbi Gembili (Dioscorea esculenta) Terhadap Karakteristik Fisiko-Kimia dan Aktivitas Prebiotik. Agritech. 2013; 33: 4.

Manaer T, Yu L, Zhang Y, Xiao XJ, Nabi XH. Anti-diabetic effects of shubat in type 2 diabetic rats induced by combination of high-glucose-fat diet and low-dose streptozotocin. J Ethnopharmacol. 2015; 169: 269–274.

Karaca T, Kara A, Nejdet S, Uslu S, Tekįner D, Yörük M. Immunohistochemical distribution of glucagon, insulin, somatostatin, gastrin, and serotonin, containing cells in the pancreas of the Van cat. Turk J Vet Anim Sci. 2010; 38: 304-311.

De D, Chatterjee K, Ali KM, Bera TK, Ghosh D. antidiabetic potentiality of the aqueous methanolic extract of seed of Swietenia mahagoni (L.). Jacq. in streptozotocin-induced diabetic male albino rat: A correlative and evidence-based approach with antioxidative and antihyperlipidemic activities. Evid Based Comp Alternat Med. 2011; 2011: 892807.

Zhang T, Gao J Jin ZY, Xu XM, Chen HQ. Protective effects of polysaccharides Lilium lancifolium on streptozotocin-induced diabetic mice. Int J Biol Macromol. 2014; 65: 436-40.

Gohari A, Ali NMA, Fahimeh ZG, Atefeh S. Urtica Dioica Distillate Regenerates Pancreatic Beta Cells in Streptozotocin-Induced Diabetic rats. Iran J Med Sci. 2018; 32(2): 174-183.

Pirmoradi L, Noorafshan A, Safaee A, Dehghani GA. Quantitative assessment of proliferative effects of oral vanadium on pancreatic islet volumes and beta cell numbers of diabetic rats. Iran Biomed J. 2016; 20: 18-25.

Tiwari BK, Pandey KB, Abidi AB, Rizvi SI. Markers of oxidative stress during diabetes mellitus. J Biomark. 2013; 2013: 378790.

Friederich M, Hansell P, and Palm F. Diabetes, oxidative stress, nitric oxide and mitochondria function. Curr Diab Rev. 2009; 5: 120–144.

Raza H and John A. Streptozotocin-induced cytotoxicity, oxidative stress and mitochondrial dysfunction in human hepatoma HepG2 cells. Int J Mol Sci. 2012; 13: 5751–5767.

Raza H, Prabu SK, John A, and Avadhani NG. Impaired mitochondrial respiratory functions and oxidative stress in streptozotocin-induced diabetic rats. Int J Mol Sci. 2011; 12: 3133–3147.

Gargari BP, Dehghan P, Aliasgharzadeh A, Jafar-abadi MA. Effects of high performance inulin supplementation on glycemic control and antioxidant status in women with Type 2 diabetes. Diab Metab. 2013; 37: 140–148.

Cani PD, Daubioul CA, Reusens B, Remacle C, Catillon G, Delzenne NM. Involvement of endogenous glucagon-like peptide-1(7–36) amide on glycaemia-lowering effect of oligofructose in streptozotocin-treated rats. J Endocrin. 2005; 185: 457–465.

Talchai C, Xuan S, Lin HV, Sussel L, Accili D. Pancreatic beta cell dedifferentiation as a mechanism of diabetic beta cell failure. Cell. 2012; 150(6): 1223–1234.

Egharevba, E., Chukwuemeke-Nwani, P., Eboh, U., & Okoye, E. Evaluation of the Antioxidant and Hypoglycaemic Potentials of the Leaf Extracts of Stachytarphyta jamaicensis (Verbenaceae). Trop J Nat Prod Res. 2023; 3(5): 170–174.