Subacute Toxicity Test of Ethanol Extract of Sungkai Leaf (Peronema Canescens Jack.) on Sgot and Sgpt Levels
http://www.doi.org/10.26538/tjnpr/v7i11.5
Keywords:
Subacute toxicity, Sungkai, SGPT, SGOT, Peronema canescens JackAbstract
Sungkai plant (Peronema canescens Jack.) is an herbal plant that people widely use traditionally to treat various diseases. Sungkai is proven to have immunostimulant, antihyperuricemia, and antibacterial activities. Due to its widespread use and to ensure its safety, it is necessary to evaluate its toxicity. This study aimed to determine the effect of variations in dose and duration of administration of ethanol extract of Sungkai leaves (Peronema canescens Jack.) on SGPT and SGOT levels in male white mice. Thirty-six mice divided into four groups (a control group and three treatment groups) of nine each were used for this study. The control group received 0.5% Na CMC suspension, and the treatment groups (II-IV) were given the ethanol extract suspension of Sungkai leaves (Peronema canescens Jack.) at a dose of 200, 400 and 800 mg/kg BW p.o, respectively. The extracts were administered daily to the experimental animals for 7, 14, and 21 days. SGPT and SGOT levels were examined on days 8, 15 and 22 using a 5010 v5+ photometer. Data analysis was performed using the SPPS with a two-way ANOVA test based on variations in dose and duration of administration. The results of this study indicate that the ethanol extract of Sungkai leaves has no significant effect on SGPT and SGOT levels (p>0.05) based on variations in the dose and duration of administration.References
Panjaitan S, Nuraeni Y. Prospek dan Teknik Budidaya Sungkai (Peronema canescens Jack.) di Kalimantan Selatan. 2014;7(1):25–9.
Yani AP, Yenita Y, Ansori I, Irwanto R. The Potential Test of Sungkai Young Leaves (Peronema canescens) to Maintain Goodhelth (Immunity) in Mice (Mus musculus). Seminar Nasional XI Pendidikan Biologi FKIP UNS; 2013. p. 245-50.
Syofyan S, Almahdy A1, Wulandari A, Alen Y, Diliarosta S, Kurniawan H, Noverial N, Putra PP, Dillasamola D. Effects of Ethanol Extract of Sungkai (Peronema canescens Jack.) on Fertility of Female Wistar Mice (Mus musculus L.). Trop J Nat Prod Res. 2023;7(3):2547–50.
Dillasamola D, Aldi Y, Wahyuni FS, Rita RS, Dachriyanus, Umar S, dkk. Study of Sungkai (Peronema canescens, Jack) leaf extract activity as an immunostimulators with in vivo and in vitro methods. Pharmacogn J. 2021;13(6):1397–407.
Latief M, Lasmana Tarigan I, Sari PM, Aurora FE. Antihyperuricemia Activity of Ethanol Extract of Sungkai Leaves-(Peronema canescens Jack) in Male White Mice. Pharm J Indones. 2021; 18 (1), 23-37.
Fransisca D, Kahanjak DN, Frethernety A. Test of the antibacterial activity of ethanol extract of sungkai leaves (Peronema canescens Jack) on the growth of Escherichia coli using the Kirby-Bauer disk diffusion method. JPLB. 2020;4(1):460–70.
Elfita, Oktiansyah R, Mardiyanto, Widjajanti H, Setiawan A. Antibacterial and antioxidant activity of endophytic fungi isolated from Peronema canescens leaves. Biodiversitas. 2022;23(9):4783–92.
Sari Lork. Utilisation of Traditional Medicine Considering Its Benefits and Safety. Pharm Sci Res. April 2006;3(1):1–7.
World Health Organization. WHO Traditional Medicine Strategy. 2014-2023.
Zhang J, Onakpoya IJ, Posadzki P, Eddouks M. The Safety of Herbal Medicine: From Prejudice to Evidence. eCAM. 2015. DOI: 10.1155/2015/316706
Sumayyah S, Salsabila N. Traditional Medicine: Between Benefits and Side Effects. Traditional Medicine: Between Benefits and Side Effects. Pharm Mag. 2017;2(5).
Boullata JI, Nace AM. Safety Issues With Herbal Medicine. Pharmacotherapy. 2000; 20, 257–69.
Kubde S, Renuka Vihar M. Adverse Drug Reactions and Pharmacovigilance of Herbal Medicines in India. Int J. Green Pharm. 2016;10(1):29–32.
Teschke R, Glass X, Schulze J. Herbal Hepatotoxicity by Greater Celandine (Chelidonium majus): Causality Assessment of 22 Spontaneous Reports. Regul. Toxicol. Pharmacol. 2011;61(3):282–91.
Williams PL, James RC, Roberts SM. Principles of Toxicology : Environmental and Industrial Applications. Wiley; 2000. p 603.
Lee J, Shin JS, Kim MR, Byun JH, Lee SY, Shin YS, dkk. Liver Enzyme Abnormalities In Taking Traditional Herbal Medicine In Korea: A Retrospective Large Sample Cohort Study Of Musculoskeletal Disorder Patients. J Ethnopharmacol. 29 Mei 2015;169:407–12.
Rowe RC SPQ. Handbook of Pharmaceutical Excipients (6th ed). 6 ed. USA: Pharmaceutical Press; 2009. p 855.
Wang HL, Chu CH, Tsai SJ, Yang RJ. Aspartate Aminotransferase and Alanine Aminotransferase Detection on Paper-Based Analytical Devices with Inkjet Printer-Sprayed Reagents. Micromachines (Basel). 2016;7(1).
Bergmeyer HU. Approved Recommendation on IFCC Methods for the Measurement of Catalytic Coencentration of Enzymes. LClin Chem Clin Biochem. 1986;24:481–95.
Andriani Y. Toxicity of Steroid Active Fraction of Dutch Teak Leaf Extract (Guazuma Ulmifolia Lamk.) on Serum Glutamate Oxalate Transaminase (SGOT) and Serum Glutamate Pyruvate Transaminase (SGPT) Activities in White Rats. Gradient Journal. 2008; 4(2): 365-371.
![](https://tjnpr.org/public/journals/1/article_3001_cover_en_US.jpg)
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 Tropical Journal of Natural Product Research (TJNPR)
![Creative Commons License](http://i.creativecommons.org/l/by-nc-nd/4.0/88x31.png)
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.