Synthesis of Endophytic Fungi Metabolites, Antimicrobial Potentials, and Detection of their Bioactive Molecules Using Gas Chromatography-Mass Spectrometry doi.org/10.26538/tjnpr/v6i4.18
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Abstract
Endophytes are recognized for their potential as producers of natural bioactive metabolites because of the diversity of products naturally synthesized and the ease with which they grow. Endophytes have a promising perspective on drug development to reduce the challenges of antimicrobial resistance in conventional drugs. This study concentrated on isolating fungal endophytes from five herbal plants, namely: Carica papaya, Jatropha gossypifolia, Moringa oleifera, Mangifera indica, and Ocimum gratissimum. Two strains of Aspergillus niger, A. amstelodami, and Rhodotorula mucilaginosa were the fungal endophytes isolated. Each of the fungi endophytes was submerged in a fermentation setup containing Potato dextrose broth (PDB) for the synthesis of their crude metabolites. Different bioactive molecules in the crude metabolites were identified using the Gas chromatography-mass spectrometry (GC-MS) method.
The antimicrobial potential of the synthesized four crude metabolites were further determined against three (3) clinical isolates: Staphylococcus aureus, Enterobacter cloacae, and Candida albicans. Inhibition zones ranged between 20±0.32 mm and 35±1.23 mm were observed and recorded for all the four crude metabolites tested against the clinical isolates mentioned above. All the crude metabolites showed to have possessed quantifiable bioactive molecules, which can be applied further in pharmaceutical processes.
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