Evaluation of The Toxic Effects of Ethanolic Leaf Extract of Tapinanthus cordifolius in Mice and Rats
Keywords:sub-chronic toxicity, Acute toxicity, safety profile, Tapinanthus cordifolius
Tapinanthus cordifolius (TC), a hemiparasitic plant in the Loranthaceae family and the Tapinanthus genus, possesses numerous medicinal properties. In order to establish its medicinal quality and toxicity profile for appropriate application in traditional medicine. This study was designed to determine the safety profile of Tapinanthus cordifolius. Oral acute and sub-chronic toxicity studies of the ethanolic extract of Tapinanthus cordifolius were evaluated. The OECD (No. 423) limit test was followed to determine the LD50 in Swiss mice, while for the sub-chronic toxicity in Wistar rats, the OECD 407 guideline was followed. Acute toxicity studies in rats revealed no mortality or observable toxicity symptoms at doses of 2000, 5000 mg/kg for a 14-day observation period. The 28-day toxicity tests of the extract showed no significant changes in body weight, food and water intake, urine output and faecal output. Haematological parameters, kidney function indices, and liver function parameters showed no significant effect after treatment with Tapinanthus cordifolius. Histological examination of various organs showed no changes in the normal architecture of the cells in the tissues of the organs. Thus, based on the results, Tapinanthus cordifolius is considered relatively safe when administered orally at the dose tested.
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