Evaluation of The Toxic Effects of Ethanolic Leaf Extract of Tapinanthus cordifolius in Mice and Rats http://www.doi.org/10.26538/tjnpr/v7i10.37

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Published: Oct 29, 2023
Keywords:
sub-chronic toxicity, Acute toxicity, safety profile, Tapinanthus cordifolius

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Amarachi Chike-Ekwughe
Department of Biochemistry, College of Science and Technology, Covenant University, Ota, Ogun State, Nigeria.
Lucy B. John-Africa
Department of Pharmacology and Toxicology, National Institute for Pharmaceutical and Research development, Idu Industrial Area, Abuja, Nigeria.
Abiodun H. Adebayo
Department of Biochemistry, College of Science and Technology, Covenant University, Ota, Ogun State, Nigeria.
Olubanke O. Ogunlana
Department of Biochemistry, College of Science and Technology, Covenant University, Ota, Ogun State, Nigeria.

Abstract

Tapinanthus cordifolius (TC), a hemiparasitic plant in the Loranthaceae family and the Tapinanthus genus, possesses numerous medicinal properties. In order to establish its medicinal quality and toxicity profile for appropriate application in traditional medicine. This study was designed to determine the safety profile of Tapinanthus cordifolius. Oral acute and sub-chronic toxicity studies of the ethanolic extract of Tapinanthus cordifolius were evaluated. The OECD (No. 423) limit test was followed to determine the LD50 in Swiss mice, while for the sub-chronic toxicity in Wistar rats, the OECD 407 guideline was followed. Acute toxicity studies in rats revealed no mortality or observable toxicity symptoms at doses of 2000, 5000 mg/kg for a 14-day observation period.  The 28-day toxicity tests of the extract showed no significant changes in body weight, food and water intake, urine output and faecal output. Haematological parameters, kidney function indices, and liver function parameters showed no significant effect after treatment with Tapinanthus cordifolius.  Histological examination of various organs showed no changes in the normal architecture of the cells in the tissues of the organs. Thus, based on the results, Tapinanthus cordifolius is considered relatively safe when administered orally at the dose tested.

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How to Cite
Chike-Ekwughe, A., John-Africa, L. B., Adebayo, A. H., & Ogunlana, O. O. (2023). Evaluation of The Toxic Effects of Ethanolic Leaf Extract of Tapinanthus cordifolius in Mice and Rats: http://www.doi.org/10.26538/tjnpr/v7i10.37. Tropical Journal of Natural Product Research (TJNPR), 7(10), 4965-4972. https://tjnpr.org/index.php/home/article/view/2850
Issue
Vol. 7 No. 10 (2023): Tropical Journal of Natural Product Research (TJNPR)
Section
Articles
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Author Biography

Amarachi Chike-Ekwughe, Department of Biochemistry, College of Science and Technology, Covenant University, Ota, Ogun State, Nigeria.

Department of Biochemistry, Faculty of Computing and Applied Sciences, Baze University, Abuja, Nigeria.

References

Adesina SK, Illoh HC, Johnny II, Jacobs IE. African mistletoes (Loranthaceae); ethnopharmacology, chemistry and medicinal values: an update. Afr J Tradit Complement Altern Med. 2013; 10(4):161-170.

Chike-Ekwughe A, Adegboyega AE, Johnson TO, Adebayo AH, Ogunlana OO. In vitro and in silico inhibitory validation of Tapinanthus cordifolius leaf extract on alpha-glucosidase in the management of type 2 diabetes. J Biomol Struct Dyn. DOI: 10.1080/07391102.2023.2212791.

Ogunmefun OT, Fasola TR, Saba AB, Oridupa OA. The ethnobotanical, phytochemical and mineral analyses of Phragmanthera incana (Klotzsch), a species of mistletoe growing on three plant hosts in South-Western Nigeria. Int J Biomed Sci. 2013; 9:37-44.

Jadhav N, Patil CR, Chaudhari KB, Wagh JP, Surana SJ, Jadhav RB. Diuretic and natriuretic activity of two mistletoe species in rats. Pharmacognosy Res. 2010; 2(1):50-57.

Saleh, I., Maigandi, S.A., Hudu, M.I., Abubakar, M.I. and Shehu, A.U. Uses and chemical composition of Misletoe (Viscum album) obtained from different species. Dutse J. Agric. Food Sec. 2015; 2: 8-12.

Egbewande OO, Jimoh AA, Ibitoye EB, Olorede BR. Utilization of African mistletoe (Tapinanthus bangwensis) leaf meal by broiler chickens. Pakistan J Nutr. 2011; 10:19-22.

Obatomi DK, Bikomo EO, Temple VJ. Anti-diabetic properties of the African mistletoe in streptozotocin-induced diabetic rats. J Ethnopharmacol. 1994; 43:13-17.

Amarachi CE, Olamilekan AN, Adamu A, Humphery AA, Olujoke OO. Comparative Phytochemical Contents and Antioxidant Activities of Tapinanthus cordifolius and Irvingia wombolu Leaf Extracts. In: Isibor PO, Akinduti P, Oranusi SU, Popoola JO, editors. Biotechnological Approaches to Sustainable Development Goals. Springer, Cham. https://doi.org/10.1007/978-3-031-33370-5_15.

Oyeniran OH, Ademiluyi AO, Oboh G. African mistletoe (Tapinanthus bangwensis Lor.) infestation improves the phenolic constituents, antioxidative and antidiabetic effects of almond (Terminalia catappa Linn.) host leaf in sucrose-rich diet-induced diabetic-like phenotypes in fruit fly. Food Front. 2021; 2:77-90.

Evbuomwan SA, Omotosho OE, Akinola OO. A Mini Review on Some Known Medicinal Uses of Tridax procumbens. Trop J Nat Prod Res. 2023;7(8):3573-84.

Ibrahim JA, Egharevba HO, Iliya I, Tarfa F, Ayodele AE. Chemical profiles as chemotaxonomic tools for Loranthaceae in Nigeria. Afri J Plan Sci. 2014;8(7):343-52.

SE, Okhale, G. E. Ugbabe, P. O. Oladosu, Ib-rahim JA, H. O. Egharevba, O. F. Kunle, E. P. Elisha, A. J. Chibuike, and U. O. Ettah. Chemical constituents and antimicrobial activity of the leaf essential oil of Ixora coccinea L (Rubiaceae) collected from North Central. Int J Bioassays. 2018; 5:5630-5637.

Chike-Ekwughe A, Adegboyega AE, Johnson TO, Adebayo AH, Ogunlana OO. In vitro and in-silico inhibitory validation of Tapinanthus cordifolius leaf extract on alpha-amylase in the management of type 2 diabetes. Inf in Med Unl. 2023; 36:101148.

OECD. OECD Guidelines for the Testing of Chemicals. OECD. 2001; 1-14.

Egbuji JV, Ejere VC, Ugwu GC, Ugwu CL. Effects of Aqueous Leaf Extracts of Loranthus micranthus Linn. on Biochemical Profile of Wistar Rats Infected with Trypanosoma brucei brucei. Tro J Nat Prod Res. 2023; 1:7(6).

Zakerkish M, Jenabi M, Zaeemzadeh N, Hemmati AA, Neisi N. The effect of Iranian propolis on glucose metabolism, lipid profile, insulin resistance, renal function and inflammatory biomarkers in patients with type 2 diabetes mellitus: A randomized double-blind clinical trial. Sci Rep. 2019; 9(1):1-11.

Asuquo GO, Udonkang MI, Oboma YI, Bassey IE, Beshe BS, Okechi OO, Owo DU, Effiong GS. Gongronema latifolium Ethanol Crude Leaf Extract Ameliorates Organ Injuries Via Anti-Inflammatory and Anti-Diabetic Mechanisms in Streptozotocin-Induced Diabetic Rats. Trop J Nat Prod Res. 2023;7(8):3823-8.

Sharif A, Ashraf M, Javeed A, Anjum AA, Akhtar MF, Akhtar B, Saleem A. Oxidative stress responses in Wistar rats on subacute exposure to pharmaceutical waste water. Environ Sci Pollut Res. 2016;23(23):24158-65.

Haggag MW, Abou El Ella SM, Abouziena HF. Phytochemical analysis, antifungal, antimicrobial activities and application of Eichhornia crassipes against some plant pathogens. Planta Daninha. 2017; 35: p. e17159560.

Banerjee P, Eckert AO, Schrey AK, Preissner R. ProTox-II: a webserver for the prediction of toxicity of chemicals. Nucleic Aci Res. 2018; 46(W1): W257-63.

Johnson TO, Istifanus G, Kutshik RJ. In vitro and in vivo analysis of the anti-plasmodial activity of ethanol extract of Phyllanthus nivosus W. Bull leaf. J Parasit Dis. 2020; 44:166-173.

Miras-Moreno B, Sabater-Jara AB, Pedreño MA, Almagro L. Bioactivity of phytosterols and their production in plant in vitro cultures. J Agric Food Chem. 2016; 28;64(38):7049-58.

Kritchevsky D, Chen SC. Phytosterols-health benefits and potential concerns: A review. Nutr Res. 2005; 25:413-428.

Ntie-Kang F. An in silico evaluation of the ADMET profile of the Streptome DB database. Spri plus. 2013; 2:1-11.

Bello I, Bakkouri AS, Tabana YM, Al-Hindi B, Al-Mansoub MA, Mahmud R, Asmawi MZ. Acute and sub-acute toxicity evaluation of the methanolic extract of Alstonia scholaris stem bark. Med Sci. 2016; 4(1):4.

Ozer J, Ratner M, Shaw M, Bailey W, Schomaker S. The current state of serum biomarkers of hepatotoxicity. Toxicol. 2008; 245(3):194-205.

Ranawat L, Bhatt J, Patel J. Hepatoprotective activity of ethanolic extracts of bark of Zanthoxylum armatum DC in CCl4 induced hepatic damage in rats. J Ethnopharmacol. 2010; 127:777-780.

Balogun FO, Tshabalala NT, Ashafa AOT. Antidiabetic medicinal plants used by the Basotho tribe of Eastern Free State: a review. J Diab Res. 2016; 34.

Adigwe OP, John-Africa LB, Adzu B, Ajoku GA, Danraka AM, Ibrahim JA. Evaluation of the toxic effects of the aqueous extract of Niprineem tea in mice and rats. Int J Biol Chem Sci. 2021; 15(5), 1979-1990.