Validation of HPLC Method for Quantifying 1,4-Naphthoquinone in Ethanol and Water Extracts of Eleutherine bulbosa http://www.doi.org/10.26538/tjnpr/v7i10.12
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Abstract
Eleutherine bulbosa (E. bulbosa) is a traditional medicinal plant known to contain 1,4-naphthoquinone as the active compound responsible for its pharmacological effects. Ensuring consistent pharmacological effects in pharmaceutical preparations necessitates the quantitative measurement of the active compound content in the extract. This study aims to establish a method for quantifying the levels of 1,4-naphthoquinone in both ethanol and water extracts of E. bulbosa and to determine the optimal levels in both extracts. The High-Performance Liquid Chromatography (HPLC) method was employed to assess the 1,4-naphthoquinone levels. Method validation was conducted to evaluate the accuracy, precision, linearity, and selectivity of the method. The validation results indicate that this method has met the standards, with the linearity test yielding an “r” value of 0.9939, the selectivity test indicating selectivity at 3.507 minutes, the precision test resulting in a %RSD of 0.7159%, and the accuracy test showing a % recovery of 99.53%. These method validation parameters satisfy the required criteria, affirming the reliability of the method employed. The average levels of 1,4-naphthoquinone in the ethanol extract were determined to be 4.5797 ppm, while in the water extract, it measured 3.2314 ppm. These findings imply that the ethanol extract contains a higher concentration of 1,4-naphthoquinone compared to the water extract. Consequently, it can be inferred that the HPLC method for quantifying 1,4-naphthoquinone levels is valid, and the ethanol extract is recommended as a superior raw material for the development of traditional medicines due to its higher content of active compounds.
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References
Mutiah R, Sari RA, Firsyaradha WY, et al. Activity and Toxicity of Eleutherine palmifolia (L.) Merr. Extract on BALB/c Mice Colitis-Associated Colon Cancer Model. Asian Pac J Cancer Prev APJCP. 2020;21(12):3579-3586. doi:10.31557/APJCP.2020.21.12.3579
Insanu M, Kusmardiyani S, Hartati R. Recent Studies on Phytochemicals and Pharmacological Effects of Eleutherine Americana Merr. Procedia Chem. 2014;13:221-228. doi:10.1016/j.proche.2014.12.032
Xin R, Shen B, Huang ZY, Liu JB, Li S, Jiang GX, Zhang, J, Cao, YH, Zou, DZ., Li, W, Li, CG, Ma, YS, Fu, D, Research Progress in Elucidating the Mechanisms Underlying Resveratrol Action on Lung Cancer. Curr Pharm Biotechnol. 2023;24(3):427-437. doi:10.2174/1389201023666220818085945
Mahabusarakam W, Hemtasin C, Chakthong S, Voravuthikunchai SP, Olawumi IB. Naphthoquinones, Anthraquinones and Naphthalene Derivatives from the Bulbs of Eleutherine americana. Planta Med. 2010;76(4):345-349. doi:10.1055/s-0029-1186143
Mutiah R, Minggarwati TS, Kristanti RA, Susanti E. Compound Identification and Anticancer Activity of Ethyl Acetate Fraction from Bawang Sabrang (Eleutherine palmifolia (L.) Merr.) on HeLa Cervical Cancer Cell Line. Indones J Cancer Chemoprevention. 2019;10(3):131-139. doi:10.14499/indonesianjcanchemoprev10iss3pp131-139
Shivatare RS, Nagore DH, Nipanikar SU. HPTLC’an important tool in standardization of herbal medical product: A review. J Sci Innov Res. 2013;2(6):1086-1096.
Nafiu MO, Hamid AA, Muritala HF, Adeyemi SB. Chapter 7 - Preparation, Standardization, and Quality Control of Medicinal Plants in Africa. In: Kuete V, ed. Medicinal Spices and Vegetables from Africa. Academic Press; 2017:171-204. doi:10.1016/B978-0-12-809286-6.00007-8
Jamshidi-Kia F, Lorigooini Z, Amini-Khoei H. Medicinal plants: Past history and future perspective. J Herbmed Pharmacol. 2017;7(1):1-7. doi:10.15171/jhp.2018.01
Sahu PK, Ramisetti NR, Cecchi T, Swain S, Patro CS, Panda J. An overview of experimental designs in HPLC method development and validation. J Pharm Biomed Anal. 2018;147:590-611. doi:10.1016/j.jpba.2017.05.006
Kabiri M, Rezadoost H, Ghassempour A. A comparative quality study of saffron constituents through HPLC and HPTLC methods followed by isolation of crocins and picrocrocin. LWT. 2017;84:1-9.
Swarnkar P, Gupta MK, Maheshwari M. Analytical Method Validation Of Compendial Hplc Method For Pharmaceuticals As Per Recent Usp And Ich Guidelines. Published online 2021.
Sangster T, Major H, Plumb R, Wilson AJ, Wilson ID. A pragmatic and readily implemented quality control strategy for HPLC-MS and GC-MS-based metabonomic analysis. Analyst. 2006;131(10):1075-1078.
Valentão P, Andrade PB, Areias F, Ferreres F, Seabra RM. Analysis of vervain flavonoids by HPLC/diode array detector method. Its application to quality control. J Agric Food Chem. 1999;47(11):4579-4582.
Mutiah R, Ardania W, Suryadinata A, Megawati D, Listiyana A, Wafi A., Annisa R, Annisa R. Influence of Chitosan Concentration on Characteristic of Microspheres Delivery System Prepared from Eleutherine palmifolia (L.) Merr. Extract. Pharmacogn J. 2022;14(6).
Mutiah R, Choiroh F, Annisa R, Listiyana A. Combinational effect of Eleutherine palmifolia (L.) merr extract and doxorubicin chemotherapy on HeLa cervical cancer cells. AIP Conf Proc. 2019;2120(1):070001. doi:10.1063/1.5115718
Shukla S. Freeze drying process: A review. Int J Pharm Sci Res. 2011;2(12):3061.
Annisa R, Hendradi E, Yuwono M. Analysis of 1,4 naphthoquinone in the Indonesian medical plant from extract Eleutherine palmifolia (L.) Merr by UHPLC. In: Vol 456. IOP Publishing Ltd; 2020:1-8. Accessed May 15, 2023.
Avula B, Bae, JY, Raman, V, Fantoukh, OI, Wang, YH, Osman, AG, Wang, M, Ali, Z, Khan, IA. Quantification of phenolic compounds from Fadogia agrestis and dietary supplements using UHPLC-PDA-MS. Planta Med. 2019;85(02):145-153.
Vidushi Y, Meenakshi B, Bharkatiya M. A review on HPLC method development and validation. Res J Life Sci Bioinform Pharm Chem Sci. 2017;2(6):178.
Bhardwaj SK, Dwivedia K, Agarwala DD. A review: HPLC method development and validation. Int J Anal Bioanal Chem. 2015;5(4):76-81.
Mazurek A, Jamroz J. Precision of dehydroascorbic acid quantitation with the use of the subtraction method – Validation of HPLC–DAD method for determination of total vitamin C in food. Food Chem. 2015;173:543-550. doi:10.1016/j.foodchem.2014.10.065
Pozharitskaya ON, Kosman VM, Shikov AN, Demchenko DV, Eschenko AY, Makarov VG. Comparison between HPLC and HPTLC densitometry for the determination of icariin from Epimedium koreanum extracts. J Sep Sci. 2007;30(5):708-712.
Mutiah R, Hadya, CM, Za, BM, Bhagawan WS, Annisa R, Indrawijaya YYA, Huwaida FI, Da, RR, Susilowati R, Taufik I. Metabolite Profiling of Eleutherine palmifolia (L.) Merr. By HPTLC-Densitometry and its Correlation with Anticancer Activities and In Vitro Toxicity. Indones J Pharm. 2019;30(3):157.
Yuwono M, Indrayanto G. Validation of chromatographic methods of analysis. Profiles Drug Subst Excip Relat Methodol. 2005;32:243-259.
Tahmouzi S, Khaksar R, Ghasemlou M. Development and validation of an HPLC-FLD method for rapid determination of histamine in skipjack tuna fish (Katsuwonus pelamis). Food Chem. 2011;126(2):756-761.
Križman M. A simplified approach for isocratic HPLC analysis of cannabinoids by fine tuning chromatographic selectivity. Eur Food Res Technol. 2020;246(2):315-322. doi:10.1007/s00217-019-03344-7
Bonoli M, Pelillo M, Toschi TG, Lercker G. Analysis of green tea catechins: comparative study between HPLC and HPCE. Food Chem. 2003;81(4):631-638.
Alquadeib BT. Development and validation of a new HPLC analytical method for the determination of diclofenac in tablets. Saudi Pharm J. 2019;27(1):66-70. doi:10.1016/j.jsps.2018.07.020
Wen D, Li C, Di H, Liao Y, Liu H. A universal HPLC method for the determination of phenolic acids in compound herbal medicines. J Agric Food Chem. 2005;53(17):6624-6629.
Wichitnithad W, Jongaroonngamsang N, Pummangura S, Rojsitthisak P. A simple isocratic HPLC method for the simultaneous determination of curcuminoids in commercial turmeric extracts. Phytochem Anal. 2009;20(4):314-319.
Franeta JT, Agbaba D, Eric S, Pavkov S, Aleksic M, Vladimirov S. HPLC assay of acetylsalicylic acid, paracetamol, caffeine and phenobarbital in tablets. Il Farm. 2002;57(9):709-713.
Shewiyo DH, Kaale E, Risha PG, Dejaegher B, Smeyers-Verbeke J, Heyden YV. HPTLC methods to assay active ingredients in pharmaceutical formulations: A review of the method development and validation steps. J Pharm Biomed Anal. 2012;66:11-23. doi:10.1016/j.jpba.2012.03.034
Bārzdiņa A, Paulausks A, Bandere D, Brangule A. The Potential Use of Herbal Fingerprints by Means of HPLC and TLC for Characterization and Identification of Herbal Extracts and the Distinction of Latvian Native Medicinal Plants. Molecules. 2022;27(8):2555.
Dwi Lestari A, Tri Prasetyo A, Palupi T, Umayah E, Yuwono M, Indrayanto G. HPLC Determination of piracetam in tablets; Validation of the method. J Liq Chromatogr Relat Technol. 2005;28(9):1407-1416.