Validation of HPLC Method for Quantifying 1,4-Naphthoquinone in Ethanol and Water Extracts of <i>Eleutherine bulbosa</i>: http://www.doi.org/10.26538/tjnpr/v7i10.12

Roihatul Mutiah; Kartika Nibras Umairah; Abdul Wafi; Rahmi Annisa

Authors

Roihatul Mutiah Department of Pharmacy, Faculty of Medical and Health Science, Universitas Islam Negeri Maulana Malik Ibrahim Malang, East Java, Indonesia
Kartika Nibras Umairah Department of Pharmacy, Faculty of Medical and Health Science, Universitas Islam Negeri Maulana Malik Ibrahim Malang, East Java, Indonesia
Abdul Wafi Department of Pharmacy, Faculty of Medical and Health Science, Universitas Islam Negeri Maulana Malik Ibrahim Malang, East Java, Indonesia
Rahmi Annisa Department of Pharmacy, Faculty of Medical and Health Science, Universitas Islam Negeri Maulana Malik Ibrahim Malang, East Java, Indonesia

Keywords:

selectivity, linearity, precision, accuracy, Method validation

Abstract

Eleutherine bulbosa (E. bulbosa) is a traditional medicinal plant known to contain 1,4-naphthoquinone as the active compound responsible for its pharmacological effects. Ensuring consistent pharmacological effects in pharmaceutical preparations necessitates the quantitative measurement of the active compound content in the extract. This study aims to establish a method for quantifying the levels of 1,4-naphthoquinone in both ethanol and water extracts of E. bulbosa and to determine the optimal levels in both extracts. The High-Performance Liquid Chromatography (HPLC) method was employed to assess the 1,4-naphthoquinone levels. Method validation was conducted to evaluate the accuracy, precision, linearity, and selectivity of the method. The validation results indicate that this method has met the standards, with the linearity test yielding an “r” value of 0.9939, the selectivity test indicating selectivity at 3.507 minutes, the precision test resulting in a %RSD of 0.7159%, and the accuracy test showing a % recovery of 99.53%. These method validation parameters satisfy the required criteria, affirming the reliability of the method employed. The average levels of 1,4-naphthoquinone in the ethanol extract were determined to be 4.5797 ppm, while in the water extract, it measured 3.2314 ppm. These findings imply that the ethanol extract contains a higher concentration of 1,4-naphthoquinone compared to the water extract. Consequently, it can be inferred that the HPLC method for quantifying 1,4-naphthoquinone levels is valid, and the ethanol extract is recommended as a superior raw material for the development of traditional medicines due to its higher content of active compounds.

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