In Vitro Antioxidation, α-Glucosidase, and α-Amylase Inhibitory Activities of Different Solvent Extracts of Thai Traditional Diabetic Medicine


  • Supawadee Trerattanathawan Faculty of Medicine, Mahasarakham University, Maha Sarakham, 44000, Thailand
  • Teeraporn Katisart Department of Biology, Faculty of Science, Mahasarakham University, Maha Sarakham, 44150, Thailand
  • Ampa Konsue Department of Biology, Faculty of Science, Mahasarakham University, Maha Sarakham, 44150, Thailand


α-amylase, α-glucosidase, Phenolic compounds, Flavonoids, Antioxidation


Globally, interest in the treatment of diabetes mellitus with medicinal plants is growing. Treatment of diabetes mellitus using traditional recipes in the Royal Textbook of King Rama V has been widely documented. The present study was aimed at investigating the effects of the recipe extracts of a Thai traditional diabetic medicine on antioxidation, α-glucosidase, and α-amylase inhibitory activities. Recipe-I comprised 6 Thai medicinal plants without any additional medicinal material, while Recipe-II was composed of 6 Thai medicinal plants with 2 additional medicinal components (ammonium alum and potassium nitrate). Constituent plants of the 2 recipes were extracted with aqueous (H2O), 50% ethanol (HE), and 95% ethanol (E). Phytochemical screening was conducted on the various extracts to determine the total flavonoid content (TFC) and total phenolic content (TPC). Three different assays, including FRAP (ferric reducing antioxidant power), DPPH (2,2-diphenyl-1-picrylhydrazyl), and ABTS (2,2′-azino-bis-[3-ethylbenzothiazoline-6-sulfonic] acid) radical scavenging were used to evaluate the antioxidant potential of the various extracts. The inhibitory activities of the recipe extracts against α-glucosidase and α-amylase enzymes were also evaluated. The results showed that Recipe-II-E was significantly higher in both TPC and TFC. The antioxidant activity of Recipe-I-E and Recipe-II-E was more potent in DPPH and FRAP assays, while Recipe-II-E was significantly more potent in the ABTS assay. Concerning the α-glucosidase and α-amylase inhibitory activities, Recipe-I-H2O was significantly (p < 0.05) more effective among the test extracts. The findings of this study revealed that the test recipe extracts possess noticeable in vitro antioxidant potential and α-glucosidase and α-amylase inhibitory activities.


Fine Arts Department. Textbook of Medicine, Royal Issue of Rama V, Volume 2. Bangkok: Amarin Printing and

Publishing Public Company; 1999.238-239. (in Thai)

Codex Alimentarius FAO-WHO, 2018. Codex General Standard for Food Additives (GSFA, Codex STAN 192-

. Available online: (accessed on 16

April 2019).

Chainumnim S, Suksamrarn S, Jarintanan F, Jongrungruangchok S, Wannaiampikul S, Tanechpongtamb W. Sonicated extract from the Aril of Momordica cochinchinensis inhibits cell proliferation and migration in aggressive prostate cancer cells. J Toxicol. 2022;27:1-12.

Rahmatullah M, Biswas A, Haq WM, Seraj S, Jahan R. An ethnomedicinal survey of cucurbitaceae family plants used in the folk medicinal practices of Bangladesh 1. Chron Young Sci 2012; 3:212-222.

Chuyen HV, Roach PD, Golding JB, Parks SE, Nguyen MH. Optimization of extraction conditions for recovering

carotenoids and antioxidant capacity from Gac peel using response surface methodology. Int J Food Sci Technol 2017; 52:972-980.

Mazzio E, Badisa R, Eyunni S, Ablordeppey S, George B, Soliman KFA. Bioactivity-guided isolation of neuritogenic

factor from the seeds of the Gac plant (Momordica cochinchinensis). Evid Based Complement. Altern. Med.

; 8953958–8953958. doi: 10.1155/2018/8953958

Jung K, Chin YW, Yoon Kd, Chae HS, Kim CY, Yoo H, Kim J. Anti-inflammatory properties of a triterpenoidal glycoside from Momordica cochinchinensis in LPS-stimulated macrophages. Immunopharmacol Immunotoxicol 2013; 35:8-14.

Kang JM, Kim N, Kim B, Kim JH, Lee BY, Park JH, Lee MK, Lee HS, Kim JS, Jung HC, Song IS. Enhancement of

gastric ulcer healing and angiogenesis by Cochinchina momordica seed extract in rats. J Korean Med Sci 2010;


Das G, Gouda S, Kerry RG, Cortes H, Prado-Audelo ML, Leyva-Gomez G, Tsouh Fokou PV, Gutiérrez-Grijalva EP,

Heredia JB, Shin HS, Patra JK. Study of traditional uses, extraction procedures, phytochemical constituents, and

pharmacological properties of Tiliacora triandra. J Chem. 2022; 7:8754528.

Rattana S, Padungkit M, Cushnie B. Phytochemical screening, flavonoid content, and antioxidant activity of

Tiliacora triandra leaf extracts. In Proceedings of the 2nd Annual International Conference of Northeast Pharmacy

Research, 2010; 60-63.

Nutmakul T, Pattanapanyasat K, Soonthornchareonnon N, Shiomi K, Mori M, Prathanturarug S. Antiplasmodial stage specific activity of tiliacorinine and yanangcorinine, and their interaction effects with chloroquine. Planta Med Lett 2016; 81(S01):P921. 1596922.

Jongchanapong A, Singharachai C, Palanuvej C, Ruangrungsi N, Towiwat P. Antipyretic and antinociceptive

effects of Ben-cha-Lo-Ka-Wi-Chian remedy. J Health Res 2010; 24(1):15-22.

Singharachai C, Palanuvej C, Kiyohara H, Yamada H, Ruangrungsi N. Pharmacognostic specification of five root

species in Thai traditional medicine remedy: Ben- Cha-LoKa-Wi-Chian. Phcog J. 2011; 3(21):1-11.

Katisart T, Rattana S. Hypoglycemic activity of leaf extracts from Tiliacora triandra in normal and

treptozotocininduced diabetic rats. Pharmacogn J. 2017; 9(5):621-625.

Liu RH, Chen SS, Ren G, Shao F, Huang HL. Phenolic compounds from roots of Imperata cylindrica var. major.

Chinese Herbal Medicines. 2013; 5(3):240-243.

Jung YK, Shin D. Imperata cylindrica: A review of phytochemistry, pharmacology, and industrial applications.

Molecules. 2021; 26(5):1454.

Padma R, Parvathy NG, Renjith V, Kalpana PR, Rahate P. Quantitative estimation of tannins, phenols and antioxidant activity of methanolic extract of Imperata cylindrica. Int J Res Pharm Sci. 2013; 4(1):73-77.

Fu L, Chen L, Liu R, Chen D. Chemical constituents of Rhizoma Imperatae and their anti-complementary activity. J. Chin. Med. Mater. 2010; 33:1871-1874.

Koh KH, Tham FY. Screening of traditional Chinese medicinal plants for quorum-sensing inhibitors activity. J.

Microbiol. Immunol. Infect. 2011; 44:144-148.

Zeng J, Liu X, Li X, Zheng Y, Liu B, Xiao Y. Daucosterol inhibits the proliferation, migration, and invasion of

hepatocellular carcinoma cells via Wnt/beta-catenin signaling. Molecules. 2017; 22:862.

Zhou XR, Wang JH, Jiang B, Shang J, Zhao CQ. A study of extraction process and in vitro antioxidant activity of total phenols from Rhizoma Imperatae. Afr. J. Tradit. Complement. Altern. Med. 2013; 10:175-178.

Yin YS, Ou J, Wei JZ, Li YQ, Li XL, Li QC, Li KH. Effects of Rhizoma Imperatae and its compound decoction on the

model of IgA nephropathy in rats. Lishizhen Med. Mater. Med. Res. 2011; 11:2659-2662

Chowdhury MS, Uddin MS, Haque F, Muhammed N, Koike M. Indigenous management of patipata (Schumannianthus dichotoma) plantation in the rural homesteads of Bangladesh. Subtrop Agric Res Dev 2007; 5(1):202-207.

Mohiuddin M, Rashid MH. Survival and growth of vegetatively grown pati-pata (Schumannianthus

dichotomas): An exploratory study. J For Sci. 1988; 17 20- 25.

Maneenoon K, Khuniad C, Teanuan Y, Saedan N, Prom-in S, Rukleng N, Kongpool W, Pinsook P, Wongwiwat W.

Ethnomedicinal plants used by traditional healers in Phatthalung Province, Peninsular Thailand. J Ethnobiology

Ethnomedicine.2015; 11:43.

Chowdhury D, Konwar BKr. Morphophenology and karyotype study of Patidoi (Schumannianthus dichotomus

(Roxb.) Gagnep. Synonym Clinogyne dichotoma Salisb. – a traditional plant of Assam. Current science. 2006; 91(5):648-651.

Kongkaneramit L, Witoonsardsilp W, Peeungvicha P, Ingkaninan K, Waranuch N, Sarisuta N. Antioxidant activity

and antiapoptotic effect of Asparagus racemosus root extracts in human lung epithelial H460 cells. Exp Ther Med.

; 2(1):143-148.

Naquvi KJ, Dohare S, Shuaib M. In vitro antioxidant activity Asparagus racemosus root. Int J Biomed Res. 2011;


Potduang B, Meeploy M, Giwanon R, Benmart Y, Kaewduang M, Supatanakul W. Biological activities of

Asparagus racemosus. Afr J Tradit Complement Altern Med.2008; 5(3):230-7.

Hayes PY, Jahidin AH, Lehmann R, Penman K, Kitching W, De Voss JJ. Structure revision of shatavarins I and IV, the major component from the roots of Asparagus racemosus. Terahedron Lett. 2006; 47:6965-6969.

Visavadiya N, Narasimhacharya RL. Asparagus root regulates cholesterol metabolism and improves antioxidant

status in hypercholesteremic rats. 2009; 6(2):219-226.

Chopra RN, Chopra IC, Handa KL, Kapur LD. Indigenous drugs of India, Calcutta, Academic Publishers, 1994; 607.

Bopana N, Saxena S. Asparagus racemosusEthnopharmacological evaluation and conservation needs. J

Ethnopharmacol 2007; 110(1):1-15.

Khare CP. Indian medicinal plants: an illustrated dictionary. Heidelberg: Springer, 2008; 72-73.

Gupta A, Chaphalka SR. Assessment of immunomodulatory activity of aqueous extract of Calamus rotang. Avicenna J Phytomed. 2017; 7(3):199-205.

Phrutivorapongkul A, Rungsimakan S. Quality evaluation of the exsiccated alum. J Thai Trad Alt Med. 2020; 18(3):573- 583.

Picheansoonthon C. Medicinal elements. 2nded. Bangkok: Amarin Printing and Publishing Public Co., Ltd. 2013; 58- 60. (in Thai)

Picheansoonthon C, Maenmas C, Jirawong V. Description of King Narai’s medicines treatise. Bangkok: Amarin Printing and Publishing Public Co., Ltd, 2001.640-641. (in Thai)

Yupparach P, Sumalee A, Konsue A. Phytochemical screening and biological activities of a remedy from A-thisa-ra-wak scripture as a folkloric diabetic medicine. Trop J Nat Prod Res. 2022; 6(6):863-867.

Konsue A, Taepongsorat L. Phytochemical screening and antioxidant activity of longevity remedy from national Thai traditional medicine scripture (formulary special edition). Trop J Nat Prod Res. 2022; 6(6):868-871.

Namwong A, Thongkrajai P, Konsue A. Effect of a Thai folk recipe on phytochemical screening, antioxidant activities, and α-Glucosidase inhibition by different solvent extracts. Phcog Res. 2020; 12:225-229.

Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: The FRAP assay. Anal Biochem. 1996; 239:70-76.

Rajurkar NS, Hande SM. Estimation of phytochemical content and antioxidant activity of some selected traditional Indian medicinal plants. Ind J Pharm Sci. 2011; 73(2):146- 151.

Yupparach P, Konsue A. Phytochemical screening and antioxidant activities of Cornukaempferia chayanii extracts and in vitro inhibitory effects on digestive enzymes. Trop J Nat Prod Res. 2022; 6(5):705-708.

Chotchoungchatchai S, Saralamp P, Jenjittikul T, Pornsiripongse S, Prathanturarug S. Medicinal plants used

with Thai traditional medicine in modern healthcare services: a case study in Kabchoeng Hospital, Surin province,

Thailand. J Ethnopharmacol 2012; 141(1):193-205.

De la Rosa LA, Moreno-Escamilla JO, Rodrigo-Garcia J, Alvarez-Parrilla E. Phenolic compounds. In E. M. Yahia &

A. Carrillo-Lopez (Eds.), Postharvest physiology and biochemistry of fruits and vegetables. 2019; 253-271.

Assefa ST, Yang EY, Chae SY, Song M, Lee J, Cho MC, Jang S. Alpha glucosidase inhibitory activities of plants with

a focus on common vegetables. Plants. 2019; 9(1):2.

Chang C, Yang M, Wen H, Chern J. Estimation of total flavonoid content in propolis by two complementary

colorimetric methods. J Food Drug Anal. 2002; 10(3):178- 82.

Singleton VL, Orthofer R, Lamuela-Raventos RM. Analysis of total phenols and other oxidation substrates and

antioxidants by means of Folin-Ciocalteu reagent. Method Enzymol. 1999; 299:152-178.

Ursini F, Mairorino M, Morazzoni P, Roveri A, Pifferi G. Antioxidant flavonoid (IdB 1031) affecting molecular

mechanisms of cellular activation. Free Rad Biol Med. 199 ;16:547-553.

Long LH and Halliwell B. Oxidation and generation of hydrogen peroxide by thiol compounds in commonly used cell culture media. Biochem Biophys Res Commun. 2001; 286:991-994.



How to Cite

Trerattanathawan, S., Katisart, T., & Konsue, A. (2023). In Vitro Antioxidation, α-Glucosidase, and α-Amylase Inhibitory Activities of Different Solvent Extracts of Thai Traditional Diabetic Medicine: Tropical Journal of Natural Product Research (TJNPR), 7(10), 4146–4151. Retrieved from

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