Saccharomyces cerevisiae Strain – Growth Kinetics, Extracellular Enzymes and Production of Fermentable Sugars from a Range of Lignocellulose Residues doi.org/10.26538/tjnpr/v5i12.19
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Abstract
Lignocellulosic materials are abundant in Nigeria and can be exploited for the production of industrially important enzymes by microorganisms. In this study, capacity of isolated Saccharomyces cerevisiae in producing crude enzymes and hydrolysis of selected renewable substrates – corn husks (CH) and Opete bagasse (OB) for sugar release was investigated. Fourier Transform Infrared Spectrophotometer (FTIR) was used to study the changes in the structures of the lignocellulosic substrates (CH and OB). High-Performance Liquid Chromatography (HPLC) was employed to study the effect of pretreatment on sugar release from CH and OB substrates. cerevisiae gave the highest cellulase (18.61 ± 2.35 U/gds), xylanase (54.79 ± 3.58 U/gds) and ligninase (110.79 ± 6.19 U/gds) activities after 6-day fermentation (SSF) at 28 ± 2oC. Maximum glucose production (44.83 ± 1.24 mg/gds) and (49.73 ± 1.88 mg/gds) respectively from pretreatment of opete bagasse (ENZTOB) and corn husk (ENZTCH) were obtained. Maximum glucose of 35.90 ± 1.24 mg/gds and 37.87 ± 2.49 mg/gds were obtained from untreated opete bagasse (ENZOB), and untreated corn husk (ENZCH), respectively. Inoculum/cell suspension released lower glucose levels – 28.887 ± 2.03 mg/gds and 38.362 ± 1.36 mg/gds for opete bagasse (IOB) and for corn husk (ICH) respectively. The xylose sugars obtained from pre-treated opete bagasse (ENZTOB), and corn husk (ENZTCH) were 55.91 ± 3.02 mg/gds and 42.38 ± 2.44 mg/gds, respectively and are higher than 24.364 ± 1.75 mg/gds and 29.349 ± 1.74 mg/gds obtained for untreated IOB and ICH, respectively. The yeast strain may find application in consolidated bioprocessing of cellulosic mass.
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