Isolation and Characterization of Some Flavonoids from the Leaves of Globimetula braunii (Loranthaceae) Growing on Terminalia catappa L. (Combretaceae)
doi.org/10.26538/tjnpr/v5i12.15
Keywords:
Globimetula braunii, Loranthaceae, Flavonols, Nuclear Magnetic ResonanceAbstract
Isolation and purification of natural products from medicinal plants are important components of drug development. Many important phytochemical compounds have been isolated from medicinal plants and are used as active therapeutic substances. This study aimed to isolate and characterize some of the phytochemical compounds present in the n-butanol soluble fraction of the ethanol extract of the leaf of Globimetula braunii (Loranthaceae) growing on Terminalia catappa. The dried pulverized leaf of Globimetula braunii was extracted with 90% ethanol using cold maceration. The ethanol leaf extract was partitioned using n-hexane, chloroform, ethyl acetate and n-butanol. The n-butanol fraction was subjected to silica gel column chromatography. The major fractions were further purified using Sephadex gel filteration. Three flavonols; rhamnetin, quercetin and rhamnetin-3-O-α-L-rhamnopyranoside were isolated. The structures of these compounds were established by spectroscopic analysis including 1D and 2D NMR. This is the first report of the isolation of these compounds from Globimetula braunii (Loranthaceae) growing on Terminalia catappa.
References
Visht S and Chaturvedi S. Isolation of natural products. JCPR. 2012; 2(3):584-599.
Sarker SD and Nahar L. An introduction to natural products isolation. In: Sarker SD, Latif Z, Gray A.I (Eds.). Natural products isolation: Totowa New Jersy: Humana Press. 2012;1-25p.
Zhang QW, Lin LG, Ye WC. Techniques for extraction and isolation of natural products: A comprehensive review. Chinese med. 2018; 13(1):1-26.
Sticher O. Natural product isolation. Natural product reports. 2008; 25(3):517-54.
Kabera JN, Semana E, Mussa AR, He X. Plant secondary metabolites: biosynthesis, classification, function and pharmacological properties. J Pharm Pharmacol. 2014; 2(7):377-92.
Velu G, Palanichamy V, Rajan AP. Phytochemical and pharmacological importance of plant secondary metabolites in modern medicine. In: Bio-organic Phase in Natural Food: An Overview. Springer, Cham. 2018; 135-156p.
Hussein RA, El-Anssary AA. Plants secondary metabolites: the key drivers of the pharmacological actions of medicinal plants. In: Builders PF (Eds.). Herbal Medicine.United Kingdom: Intechopen Limited; 2019; 11-30p https://doi.org/10.5772/intechopen.76139
Aliyu MM, Musa AI, Kamal MJ, Mohammed MG. Phytochemical screening and anticonvulsant studies of ethyl acetate fraction of Globimetula braunii on laboratory animals. Asian Pac J of Trop Biomed. 2014; 4(4):285-9.
Burkill HM. The useful plants of West Tropical Africa, Volume 2. (2nd ed.). Families AD. Royal Botanic Gardens; 1985.
Atiku I, Sule MI, Pateh UU, Musa AM, Ya’u J, Sani YM, Hanwa UA, Abdullahi SM, Adamu SA, Lawal EA, Abdulrahman H. Phytochemical, anti-inflammatory and analgesic studies of the crude ethanolic leaf extract of Globimetular brounii Van Tieghem (Family: Loranthaceae). Nat Prod Chem Res. 2015; 3(6):1-4.
Okpanachi GO, Oyi AR, Musa H, Abdulsamad A, Sani MB, Ya’u J. Phytochemical Screening and HypoglycemicProperty of Globimetula braunii (Loranthaceae) Leaf Extracts. J Pharm Res Int. 2018; 22(1):1-11.
Durojaye AM, Oluwabunmi OA, Olatubosun FK, Joseph AA. Two antihyperglycaemic compounds from Globimetula braunii (Engl.) Van Tiegh (Loranthaceae). GSC Bio Pharm Sci. 2020; 11(1):046-054.
Ja’afar MK, Jamil S, Basar N. Antioxidant activity of leaf extracts of Globimetula braunii (Engler) van Tiegh parasitizing on Piliostigma thonningii and Parkia biglobosa. Jurnal Teknologi. 2017; 79(5):43-47.
Ja'afar MK, Jamil S, Basar N, Bakar MB, Sarker SD, Flanagan KJ, Senge MO. Lactones and Flavonoids isolated from the Leaves of Globimetula braunii. Nat Prod Commun.2017; 12(9):1455-1458.
Mabry T, Markham KR, Thomas MB. The systematic identification of flavonoids. (2nd ed.). New York: SpringerScience & Business Media; 2012; 41-43p.
Workman J and Workman J. Handbook of Organic Compounds: Methods and interpretations. Volume 1. University of Michigan: Academic Press; 2001. 115-127 p.
Fukunaga T, Nishiya K, Kajikawa I, Watanabe Y, Suzuki N, Takeya K, Itokawa H. Chemical studies on the constituents of Hyphear tanakae Hosokawa from different host trees. Chem Pharm Bull. 1988; 36(3):1180-1184.
Harvala E, Exner J, Becker H. Flavonoids of Loranthus europaeus. J Nat Prod. 1984; 47(6):1054-1055.
Kim YK, Kim YS, Choi SU, Ryu SY. Isolation of flavonol rhamnosides from Loranthus tanakae and cytotoxic effect of them on human tumor cell lines. Arch Pharm Res. 2004; 27(1):44-47.
Olennikov DN and Chirikova NK. Rhamnetin glycosides from the genus Spiraea. Chem Nat Comp. 2018; 54(1):41-45.
Jiang JG, Huang XJ, Chen J, Lin QS. Comparison of the sedative and hypnotic effects of flavonoids, saponins, and polysaccharides extracted from Semen Ziziphus jujube. Nat Prod Res. 2007; 21(4):310-320.
Jäger AK and Saaby L. Flavonoids and the CNS. Molecules. 2011; 16(2):1471-1485.
Datta BK, Datta SK, Chowdhury MM, Khan TH, Kundu JK, Rashid MA, Nahar L, Sarker SD. Analgesic, antiinflammatory and CNS depressant activities of sesquiterpenes and a flavonoid glycoside from Polygonum viscosum. Pharmazie. 2004; 59(3):222-225.
Fernández SP, Wasowski C, Loscalzo LM, Granger RE, Johnston GA, Paladini AC, Marder M. Central nervous system depressant action of flavonoid glycosides. Eur J Pharmacol. 2006; 539(3):168-176.
Lan L, Wang Y, Pan Z, Wang B, Yue Z, Jiang Z, Li L, Wang C, Tang H. Rhamnetin induces apoptosis in human breast cancer cells via the miR-34a/Notch-1 signaling pathway. Oncol Lett. 2019; 17(1):676-682.
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