Penicillium citrinum: A Promising Candidate for Protease Production
ttp://www.doi.org/10.26538/tjnpr/v7i9.37
Keywords:
Penicillium, Halozones, Enzyme ActivitY, Immobilization, Fungi, ProteaseAbstract
Proteases are one of the most important classes of industrial enzymes accounting for 60% of the global industrial enzyme market. Microbes are the most preferred sources of proteases because of their rapid growth, easy genetic manipulation and limited cultivation space. The aim of this study was to isolate and characterize potent fungal candidates for protease production. Proteaseproducing fungi were isolated from abattoir soil using standard mycological methods while screening for protease production was determined by the presence of halozones on skimmed milk agar medium. Enzyme activity using best isolate was evaluated on time course and at various pH regimes (5.0-8.0) and temperatures (25-45оC). Best isolate had halozone diameter of 69 mm and identified as Penicillium citrinum by sequencing its 18S rRNA. Optimum protease activity was recordedon the 4th day for both immobilized and free spores. When pH was optimized, pH 6 (19.25± 0.32 U/mL) and pH 6.5 (14.21 ± 0.65 U/mL) were best for immobilized and free spores respectively whereas 35оC was the best temperature condition for optimum protease activity for both immobilized (22.44 ±0.71 U/mL) and free spores (17.16 ± 0.51 U/mL). We therefore conclude that the abattoir soil vironment is a potential reservoir of protease-production fungal determinants while spore immobilization resulted in better proteaseproduction.
References
Adrio JL, Demain AL. Microbial enzymes: tools for biotechnological processes. Biomolecules. 2014 Jan 16;4(1):117-139.
Sharma KM, Kumar R, Panwar S, Kumar A. Microbial alkaline proteases: Optimization of production parameters and their properties.J. Genet. Eng. Biotechnol. 2017 Jun 1;15(1):115-126.
Sumantha A, Larroche C, Pandey A. Microbiology and industrial biotechnology of food-grade proteases: a perspective. Food Technol. Biotechnol. 2006 Mar;44(2):211.
Hassan MA, Haroun BM, Amara AA, Serour EA. Production and characterization of keratinolytic protease from new wool-degrading Bacillus species isolated from Egyptian ecosystem. Biomed Res. Int. 2013 Jan 1;2013.
Tork SE, Shahein YE, El-Hakim AE, Abdel-Aty AM, Aly MM. Purification and partial characterization of serinemetallokeratinase from a newly isolated Bacillus pumilus NRC21. Int.J. Biol. Macromol. 2016 May 1;86:189-96.
Nwokoro O EkwemOH, Oyeagu U. Extracellular Alkaline Protease production By Bacillus licheniformis in Submerged Fermentation.Ann., Food Sci. Technol..2017. Vol 18, Issue 3, 467-477
Saran S, Isar J, Saxena RK. A modified method for the detection of microbial proteases on agar plates using tannic acid. J. Bioch.Bioph Meth. 2007 Jun 10;70(4):697-9.
Nwokeoma CI, Arotupin DJ, Olaniyi OO, Adetuyi FC. Enhanced production, purification and characterization of pectinase from Aspergillus flavus. Trop J Nat Prod Res. 2021, 5 (10):1876-1882
Ametefe GD, Lemo AO, Orji FA, Kolawole LA, Iweala EE, Chinedu SN. Comparison of Optimal Fungal Pectinase Activities Using the Box-Behnken Design. Trop J Nat Prod Res. 2021, 5(9):1656-1654
.ElHadj-Ali N, Agrebi R, Ghorbel-Frikha B, SellamiKamoun A, Kanoun S, Nasri M. Biochemical and molecular characterization of a detergent stable alkaline serine-protease from a newly isolated Bacillus licheniformis NH1.Enzyme Microb Technol. 2007 Mar 5;40(4):515-23.
Josephine FS, Ramya VS, Devi N, Ganapa SB, Siddalingeshwara KG, Venugopal N, Vishwanatha T. Isolation, production and characterization of protease from Bacillus sp. isolated from soil sample. J Microbiol Biotech Res. 2012;2(1):163-8.
Chandrasekaran S, Kumaresan SS, Manavalan M. Production and optimization of protease by filamentous fungus isolated from paddy soil in Thiruvarur District Tamilnadu. J. App Biol. Biotechnol. 2015 Dec 19;3(6):066- 9.
Paula MD, Monaliza, DA.B, Perola OM. A biotechnology, perspective of fungal protease. Braz. J. Microbiol., 2015 46(2):337-346.
Joo DS, Cho MG, Lee JS, Park JH, Kwak JK, Han YH, Bucholz R. New strategy for the cultivation of microalgae using microencapsulation. J. Microencapsul. 2001 Jan 1;18(5):567-76.
Oyeagu U, Nwuche CO, Ogbonna CN, Ogbonna J C.Amylase Production by Aspergillusniger immobilized in Microporous Calcium Alginate Gel Beads. Rom Biotechnol Lett. 2021, 26(4): 2825-2833.
Maghsoodi V, Kazemi A, Nahid P, Yaghmaei S, Sabzevari MA. Alkaline protease production by immobilized cells using B. licheniformis. Sci. Iran. 2013 Jun 1;20(3):607-10.
Al-Meani S. production of protease using immobilization cells technique. Biochem. Cell. Arch. 2020, 20 (1):0000- 000
Sinha P, Singh RK, Srivastva R, Sharma R, Tiwari SP. Characterization and optimization of alkaline protease enzyme produced by soil borne bacteria. Trends Life Sci. 2013;2(2):38-46.
Nagamani A, Kunwar IK, Manoharachary C. Handbook of Soil Fun. Handbook of Soil Fun. 2006.
Ogbonna JC, Amano Y, Nakamura K. Elucidation of optimum conditions for immobilization of viable cells by using calcium alginate. J. Biosci. Bioeng, 1989 Jan 1;67(2):92-6.
Oyeagu U, Nwuche CO, Ogbonna CN, Ogbonna JC. Addition of fillers to sodium alginate solution improves stability and immobilization capacity of the resulting calcium alginate beads. Iran. J. Biotechnol.. 2018 Apr;16(1):66-72
Potumarthi R, Ch S, Jetty A. Alkaline protease production by submerged fermentation in stirred tank reactor using Bacillus licheniformis NCIM-2042: effect of aeration and agitation regimes. Biochem. Eng. J. 2007 May 1;34(2):185- 92.
Maitig, AM. Alhoot MAM. Tiwari K. Isolation and Screening of Extracellular Protease Enzyme from Fungal Isolates of Soil.J Pure Appl Microbiol, 2018, 12(4), 2059- 2067
Javed S, Meraj M, Bukhari SA, Irfan R, Mahmood S. Hyper-production of alkaline protease by mutagenic treatment of Bacillus subtilis M-9 using agroindustrial wastes in submerged fermentation. J. Microb. Biochem. Technol. 2013;5(3):74-80.
Abusham RA, Rahman RN, Salleh AB, Basri M. Optimization of physical factors affecting the production of thermo-stable organic solvent-tolerant protease from a newly isolated halo tolerant Bacillus subtilis strain Rand. Microb. Cell Fact. 2009 Dec;8(1):1-9.
Ikram-Ul-haq HM, Umber H. Production of protease by Penicillium chrysogenum through optimization of environmental conditions. J. Agric. Soc. Sci 2006;2(1):23- 25.
Maal KB, Emtiazi G, Nahvi I. Production of alkaline protease by Bacillus cereus and Bacillus polymixa in new industrial culture mediums and its immobilization. Afr J Microbiol Res. 2009 Sep 1;3(9):491-497.
Agrawal D, Patidar P, Banerjee T, Patil S. Production of alkaline protease by Penicillium sp. under SSF conditions and its application to soy protein hydrolysis. Process Biochem. 2004 Apr 30;39(8):977-81.
Braaksma M, Smilde AK, van der Werf MJ, Punt PJ. The effect of environmental conditions on extracellular protease activity in controlled fermentations of Aspergillus niger. Microbiol. 2009 Oct;155(10):3430-9.
Ganesh-Kumar AG, Nagesh N, Prabhakar TG, Sekaran G. Purification of extracellular acid protease and analysis of fermentation metabolites by Synergistes sp. utilizing proteinaceous solid waste from tanneries. Bioresour Technol. 2008 May 1;99(7):2364-72.
Morimura SK, Sonada Y. Production of protease using waste water from the manufacture of Shochu. J.Ferment.Bioeng. 199477: 183-187.
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