Chemical Composition and Biological Activities of Essential Oil from Ocimum basilicum L. Collected in Dak Lak, Vietnam

http://www.doi.org/10.26538/tjnpr/v7i9.27

Authors

  • Dam T. B. Hanh Tay Nguyen University, Buon Ma Thuot, Dak Lak 630000, Vietnam
  • Truong N. Ngu Tay Nguyen University, Buon Ma Thuot, Dak Lak 630000, Vietnam
  • Phan H. T. Bao Tay Nguyen University, Buon Ma Thuot, Dak Lak 630000, Vietnam
  • Trinh N. T. Vy Tay Nguyen University, Buon Ma Thuot, Dak Lak 630000, Vietnam
  • Nguyen T. V. Dung Tay Nguyen University, Buon Ma Thuot, Dak Lak 630000, Vietnam
  • Dang T. T. My Tay Nguyen University, Buon Ma Thuot, Dak Lak 630000, Vietnam
  • Le T. T. Loan Tay Nguyen University, Buon Ma Thuot, Dak Lak 630000, Vietnam
  • Do T. Lam Institute of Natural Products Chemistry, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Cau Giay District, Hanoi 122100, Vietnam
  • Phi H. Nguyen Institute of Natural Products Chemistry, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Cau Giay District, Hanoi 122100, Vietnam
  • Nguyen P. Khanh Department of Biochemistry, Military Hospital 175, 876 Nguyen Kiem Street, Go Vap District, Ho Chi Minh City 70000, Vietnam
  • Dao C. To Phenikaa University Nano Institute (PHENA), Phenikaa University, Yen Nghia, Ha Dong District, Hanoi 12116, Vietnam

Keywords:

antibacterial, antioxidant, trans-α-bergamotene, essential oil, eucalypto, estragole, Ocimum basilicum Linn

Abstract

Ocimum basilicum Linn (Lamiaceae), also known as sweet Basil, is a widely recognized medicinal plant. Although this species has been extensively researched on its biological activities and chemical composition in some Provinces of Vietnam, however, studies on the
chemical composition and biological properties of this species growing in Dak Lak Province have not been investigated to date. The chemical composition of the essential oil from O. basilicum, collected in Vietnam, was analyzed using the gas chromatography-coupled mass spectrometry (GC–MS) method. The essential oil content yielded 0.55%, with 34 compounds being determined. Among them, the major constituents (%) were as follows: estragole (73.66%), eucalypto (6.41%), trans-α-bergamotene (3.97%), linalool (2.25%), fenchol (1.64%), 2- isopropyl-5-methyl-9-methylene- bicyclo[4.4.0]dec-1-ene (1.47%), 1,3,6-octatriene,3,7- dimethyl-(Z) (1.36%), (+)-2-bornanone (1.34%), and methyl eugenol (1.11%). Furthermore, an antibacterial test on O. basilicum essential oil was conducted, and the results demonstrated strong antibacterial activity against Escherichia coli with an inhibition zone of 18.6 ± 0.9 mm and the lowest minimum inhibitory concentration (MIC) value (4.3 ± 0.2 mg/mL). Additionally, the antioxidant capacity of Basil essential oil was evaluated using the 2,2-diphenyl-1- picryhydrazyl (DPPH) method. The antioxidant efficiency of essential oil was determined with an IC50 of 6.5 ± 0.3 μg/mL, compared with butylated hydroxytoluene (BHT) with an IC50 value of 6.1 ± 0.1 μg/mL. These findings indicate that O. basilicum grown in Vietnam possesses a high natural potential for pharmaceutical applications.

References

Akbar S. Handbook of 200 Medicinal Plants. Ocimum basilicum L. (Lamiaceae). New York: Springer; 2020. 1313-1326 p.

Simon JE, Morales MR, Phippen WB, Vieira R, Hao Z. Basil: a source of aroma compounds and a popular culinary and ornamental herb. Perspectives on new corps and new uses. J. Janick (ed.). Virginia: ASHS Press. 1999. 499-505 p.

Akgül A. Volatile oil composition of sweet basil (Ocimum basilicum L.) cultivating in Turkey. Die Nahrung. 1989; 33(1):87-88.

Poonkodi K. Chemical composition of essential oil of Ocimum basilicum L. (BASIL) and its biological activitiesan overview. J Crit Rev. 2016; 3(3):56-62.

Zheljazkov VD, Cantrell CL, Evans WB, Ebelhar MW, Coker C. Yield and composition of Ocimum basilicum L. and Ocimum sanctum L. grown at four locations. Hortscience. 2008; 43(3):737-741.

Hussain AJ, Anwar F, Sherazi STH, Przybyiski R. Chemical composition, antioxidant and antimicrobial activities of basil (Ocimum basilicum) essential oil depends on seasonal variations. Food Chem. 2008; 108(3):986-995.

Opalachenoiva G, Obreshkova D. Comparatve studies on the activity of basil-an essential oil from Ocimum basilicum L. against multidrug resistant clinical isolates of the genra Staphylococcus, Enterococcus and Pseudomonas by using different test methods. J Microbiol Methods. 2005; 54(1):105-110.

Politeo O, Jukic M, Milos M. Chemical composition and antioxidant capacity of free volatile aglycones from basil (Ocimum basilicum L.) compared with its essential oil. Food Chem. 2007; 101(1):379-385.

Khair-ul-Bariyah S, Ahmed D, Ikram M. Ocimum basilicum: A review on phytochemical and pharmacological studies. Pakistan J Chem. 2012; 2(2):78-85.

Rubab S, Hussain I, Khan BA, Unar AA, Abbas KA, Khichi ZH, Khan M, Khanum S, Rehman KU, Khan H. Biomedical description of Ocimum basilicum L. J Islamic Int Med Coll. 2017; 12(1):59-67.

Purushothaman B, Srinivasan RP, Suganthi P, Ranganathan B, Gimbun J, Shanmugam K. A comprehensive review on Ocimum basilicum. J Nat Remedies. 2018; 18(3):71-85.

Singh D, Chaudhuri PK. A review on phytochemical and pharmacological properties of Holy basil (Ocimum sanctum L.). Ind Crops Prod. 2018; 118(S1):367-382.

Oribayo OO, Owolabi MA, Ukpo GE, Shode FO. Antioxidant activity of some Nigerian medicinal plants used in malaria treatment. Trop J Nat Prod Res. 2018; 2(1):18- 22.

Kola-Mustapha AT, Jaiyeola ET, Olufadi-Ahmed HY, Ayotunde HT, Ghazali YO. Evaluation of Zingiber officinale Rosc. And Ocimum basilicum L. essential oilsloaded gel base for the treatment of oral candidiasis. Trop J Nat Prod Res. 2020; 4(10):831-837.

Saleh RF, Gaidan1 AM, Al-Mayah QS. Green synthesis of silver nanoparticles using aqueous leaf Extract of Ocimum basilicumand investigation of their potential antibacterial Activity. Trop J Nat Prod Res. 2021; 5(1):94-99.

Hago S, Lu T, Abdelgadir AA, Yassin S, Ahmed EM, Xu H. Phytochemical constituents and in-vitro anticancer activity of some medicinal plant extracts against MCF-7 and MDA-MB-435 human breast cancer cell lines. Trop J Nat Prod Res. 2023; 7(3):2506-2515.

Quynh Anh TT, Bich Thuy DT, Thu Hang VT, Van Anh NT. Influential factors in extraction process, antioxidant and antibacterial capacity of basil (Ocimum basilicum L.) essential oil in Thua Thien Hue. National Conference on Biotechnology. 2020. 238-243 p.

Bozin B, Mimica-Dukic N, Simin N, Anackov G. Characterization of the volatile composition of essential oil of some lamiaceae species and the antimicrobial and antioxidant activities of the entire oils. J Agric Food Chem. 2006; 54(5):1822-1828.

NCCLS (National Committee for Clinical Laboratory Standards). Performance standards for antimicrobial disc susceptibility test (6th ed.) Approved Standard. M2-A6, Pennsylvania: 1997.

NCCLS (National Committee for Clinical Laboratory Standards). Performance standards for antimicrobial susceptibility testing (9th International Supplement). M100- S9, Wayne, Pennsylvania: 1999.

Tran TH, Hao Nguyen HHH, Nguyen DC, Nguyen TQ, Tan H, Hong Nhan LT, Nguyen DH, Tran DL, Do ST, Nguyen TD. Optimization of microwave - assisted extraction of essential oil from Vietnamese Basil (Ocimum Basilicum L.) using response surface methodology. Processes. 2019; 6(11):206.

Luong NX, Nhan NT, Thuy NT, Vinh TX. Chemical composition, antimicrobial and antioxidant activitites of essential oil from (Ocimum basilicum L.) collected in Thanh Hoa Province. Hong Duc Univ J Sci. 2022;12(E7):66-75.

Thanh Tuyen VT, Nhu Quyen LT. Investigation of chemical composition and antimicrobial activities of essential oil from basil (Ocimum basilicum L.) grown in Binh Dinh. Vietnam J Sci Technol. 2020; 56(3):114-119.

Hue PT, Thu Le VT, Dat VT. Chemical compositions and antimicrobial activities of basil essential oil (Ocimum basilicum L.) grown in Thai Nguyen. TNU J Sci Technol. 2023; 228(09):169-174.

Gebrehiwot H, Bachetti RK, Dekebo A. Chemical composition and antimicrobial activities of leaves of sweet basil (Ocimum basilicum L.) herb. Int J Basic Clin Pharmacol. 2015; 4(5):869-875.

De Martino L, Amato G, Caputo L, Nazzaro F, Scognamiglio MR, De Feo V. Variations in composition and bioactivity of Ocimum basilicum cv

‘Aroma 2’ essential oils. Ind Crops Prod. 2021; 172:114068.

Chalchat JC, Özcan MM. Comparative essential oil composition of flowers, leavesand stems of basil (Ocimum basilicum L.) used as herb. Food Chem. 2008; 110(2):501- 503.

Joshi RK. Chemical composition antimicrobial activity of the essential oils of Ocimum basilicum L. (sweet basil) from Western Ghats of northwest Karnataka, India. Anc Sci Life. 2014; 33(3):151-156.

Farouk A, Fikry R, Mohsen M. Chemical composition and antioxidant activity of Ocimum basilicum L. essential oil cultivated in Madinah Monawara, Saudi Arabia and its comparison to the Egyptian chemotype. J Essent Oil-Bear Plants. 2016; 19(5):1119-1128.

Leila Leila HK, Brada M, Brahmi F, Djilali A, Fauconnier ML, Lognay G. Chemical composition and antioxidant activity of essential oil of Ocimum basilicum leaves from the northern region of Algeria. J Herb Med. 2012; 1(2):53- 58.

Al Abbasy DW, Pathare N, Al-Sabahi JN, Khan SA. Chemical composition and antibacterial activity of essential oil isolated from Omani basil (Ocimum basilicum Linn.). Asian Pac J Trop Dis. 2015; 5(8):645-649.

Sienkiewicz M, Lysakowska M, Pastuszka M, Bienias W, Kowalczyk E. The potential of use basil and rosemary essential oils as effective antibacterial agents. Molecules. 2013; 18(8):9334-9351.

Published

2023-10-03

How to Cite

Hanh, D. T. B., Ngu, T. N., Bao, P. H. T., Vy, T. N. T., Dung, N. T. V., My, D. T. T., … To, D. C. (2023). Chemical Composition and Biological Activities of Essential Oil from Ocimum basilicum L. Collected in Dak Lak, Vietnam: http://www.doi.org/10.26538/tjnpr/v7i9.27. Tropical Journal of Natural Product Research (TJNPR), 7(9), 4032–4037. Retrieved from https://tjnpr.org/index.php/home/article/view/2655

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