Method Development for Simultaneous Quantification of Polyphenol Compounds in Artichoke (Cynara scolymus L.) Leaf Dry Extract by UPLC-PDA http://www.doi.org/10.26538/tjnpr/v7i9.22
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Abstract
Artichoke (Cynara scolymus L.) is renowned for its cholesterol-lowering, diuretic, liverprotective, and antioxidant attributes, primarily attributed to its abundance of caffeoylquinic acid derivatives and flavonoids. However, a deficiency remains in the quality control of bioactive ingredients within artichoke. This study's core objective was to establish and validate a ultrahigh-performance liquid chromatography-photodiode array detector (UPLC-PDA) method capable of quantifying 12 specific polyphenol compounds as 1-caffeoylquinic acid, 3- caffeoylquinic acid, chlorogenic acid or 5-caffeoylquinic acid, 4-caffeoylquinic acid, cynarine or 1,3-dicaffeoylquinic acid, 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, 1,5- dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid, caffeic acid, scolymoside and cynaroside present in both artichoke extracts and supplements. The method demonstrated commendable system suitability, specificity, and a linear range spanning 1-500 µg/mL (R2 = 0.99). Furthermore, we effectively applied this developed method to assess the polyphenol content in 19 distinct artichoke supplements. This concurrent quantification strategy offers a reliable avenue for evaluating the polyphenolic constituents across various artichoke preparations.
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