Protective Effect of Ethanol Extract of Senna occidentalis Leaf Against CadmiumInduced Hepat
https://doi.org/10.26538/tjnpr/v1i1.4
Keywords:
Hepatoprotective, Cadmium, Antioxidant, Rats, Senna occidentalisAbstract
Cadmium is a known environmental and industrial toxicant exposure to which leads to the generation of reactive oxygen species (ROS), with attendant oxidative damage to membrane lipids, cellular proteins and DNA. Recently, the use of medicinal plants as prophylactic or therapeutic agents against certain diseases has become popular. This study was aimed at assessing the hepatoprotective effect of ethanol extract of Senna occidentalis leaf in cadmium intoxicated rats. Thirty-five rats, each weighing on the average 150 ± 40 g were randomly selected into seven groups of five rats each. Each group received appropriate treatment. At the end of the treatment period, each rat was sacrificed and the antioxidant status determined using extant methods. The data collected were expressed as mean ± SD and statically analyzed using one-way analysis of variance (ANOVA). The results showed that cadmium intoxication significantly increased liver malondialdehyde (MDA) levels but caused decrease in superoxide dismutase (SOD) and catalase (CAT) activities as well as reduced glutathione levels compared to control. The level offragmented DNA also increased significantly. However, co-treatment of rats with cadmium chloride and the extract significantly reduced the concentrations MDA and the severity of the DNA fragmentation. Along with these are concomitant increase in SOD and CAT activities and GSH concentration. Ethanol extract of Senna occidentalis leaf possesses bioactive agents capable of protecting liver against cadmium-induced lipid peroxidative membrane damage. The hepatoprotection potential of the leaf extract is likely due to the boost in the organs antioxidant
capacity.
References
Lawrence, A. & Hemingway, K. (2003). Effects of Pollution on Fish: Molecular Effects and Population Responses. Oxford: Blackwell Science Ltd.
Bernet D, Schmidt H, Meier W, Burkhardt-Hol P and Wahli T. Histopathology in fish: proposal for a protocol to assess aquatic pollution. Journal of Fish Disease1999; 22: 25-34.
Di Giulio, R. T. & Hinton, D. E. (2008). The Toxicology of Fishes. London: Taylor and Francis.
Aslam M, Ahmad ST, Dayal R., Javid K, Umar S, Asiaf A, Nafees S, Bhat JU, Wani A, Samim M and Singh S. Nephroprotective action of Peucedanum grande against cadmium chloride induced renal toxicity in wistar rats. EXCLI Journal 2012; 11: 444-452
Egharevba HO, Odigwe AC, Abdullahi MS, Okwute SK and Okogun JI. Phytochemical Analysis and Broad Spectrum Antimicrobial Activity of Cassia Occidentalis L. (whole plant). New York Science Journal 2010; 3(10): 74-81.
Raghunatan, K & Mitra, R. (1982). Pharmacognosy of indigenous drugs. New Delhi: Central council for research in Ayurvedac and Siddha. 1: 529.
Kaur I, Ahmad S and Harikumar SL. Pharmacognosy, Phytochemistry and Pharmacology of Cassia occidentalis Linn. International Journal of Pharmacognosy and Phytochemical Research 2014; 6(2): 151-155.
Colle F, Kaminsky R, Nkunya MH, Ogwal EN and Brun R. Trypanocidal activity of African plants. Journal of Ethnopharmacology 2003;5: 1-11.
Odeja OO, Obi G, Ogwuche CE, Elemike EE and Oderinlo OO. Phytochemical screening, Antioxidant and Antimicrobial activities of Senna occidentalis (L.) leaves. International Journal of Herbal Medicine 2014; 2(4): 26-30.
Vijayalakshmi S, Ranjitha J, DeviRajeswari V and Bhagiyalakshmi M. Pharmacological profile of Cassia occidentalis L – A review. International Journal of Pharmacy and Pharmaceutical Sciences 2013; 5(3): 29-33.
Sharma N, Trikha P, Athar M, and Raisuddin, S. In vitro inhibition of carcinogen induced mutagenecity by cassia occidentalis and Emblica officinalis. Journal of drug and Chemical Toxicology 2000; 23: 477-484.
Bin-Hafeez B, Ahmad I, Haque R and Raisuddin S. Protective effect of Cassia occidentalis L. on cylcophosphamide-induced suppression of humoral immunity in mice. Journal of Ethnopharmacology 2001; 75: 13-18.
Emmanuel S, Rani MS and Sreekant MR. Antidiabetic activity of Cassia occidentalis Linn. In streptozotocin-induced diabetic rats: dose dependent studies. International Journal of Pharmacology and Biological Sciences 2010; 1: 15-25.
Rani MS, Emmanuel S, Sreekant MR and Ignacimuthu, S. Evaluation of in vivo antioxidant and hepatoprotective activity of Cassia occidentalis Linn. against paracetamol-induced liver toxicity in rat. International Journal of Pharmacy and Pharmaceutical Sciences 2010; 2(3): 67-70.
Bhagat M and Saxena AK. Evaluation of Cassia occidentalisfor in vitro cytotoxicity against human cancer cell lines and antibacterial activity. Indian Journal of Pharmacology 2010; 42(4): 234-237.
Sreegith G, Latha PG, Shine VJ, Anuja GI, Suja SR, Sini S, Shyamal S, Pradeep S, Shikha P and Rajasekharan S. Antiallergy, Anti inflammatory, Anti-Lipid peroxidation effect of Cassia occidentalis Linn. Indian journal of experimental Biology 2010; 48: 494-498.
Verma L, Khatri A, Kaushik B, Patil UK and Pawar RS. Antidiabetic activity of Cassia occidentalis (Linn.) in normal and alloxan-induced diabetic rats. Indian Journal of Pharmacology 2011; 42(4): 224-228.
Yuvaraj G,Venkatesh K, Gayathri S, Albert S and Rajesh KN. Antioxidant Activity of the Combined Ethanolic Extract of Derrisbrevipes and Derris indica against Carbon Tetrachloride -Induced Oxidative Stress in Wistar Rats. International Journal of Pharmaceutical and Clinical Research 2010; 2(1): 54-57
Vadnere GP, Patil AV, Jain SK and Wagh SS. Investigation on in-vitro Antioxidant activity of whole plant of Cassia occidentalis Linn. (Caesalpiniaceae). International Journal of Pharm Tech Research 2011; 3(4): 1985-1991.
Kolhapure SA and Mitra WS. Meta-analysis of 50 phase III clinical trials in evaluation of efficacy and safety of Liv.52 in infective hepatitis. Medical Update 2014; 12: 51–61.
Ravi Kumar A and Abbulu K. Antioxidant activity of ethanolic extract of Cassia occidentalis against carbon tetrachlorideinduced oxidative stress in wistar rats. International Journal of Chemical Science 2011; 9(1).
Balaraman R, Gulati OD, Rathod SP and Bhatt JD. The acute pressor response to cadmium in rats. Archives Internationales
De Pharmacodynamie Et De Thérapie 1989; 30: 254-66.
Rice-Evans C, Omorphos SC and Baysal E. Sickle cell membranes and oxidative damage. Biochemistry Journal 1986;
: 265-269.
Anderson ME. Determination of glutathione and glutathione disulfide in biological samples. Methods in Enzymology 1985;
: 548-555.
Misra HP and Fridovich I. The role of superoxide anion in the auto-oxidation of epinephrine and a simple assay for superoxide
dismutase. Journal of Biological Chemistry 1972; 247: 3170-3175.
Sinha AK. Colorimetric assay of catalase. Annal of Biochemistry 1972; 47:389-394.
Wu B, Ootani A, Iwakiri R., Sakata Y, Fujise T, Amemori S, Yokoyama F, Tsunada S and Fujimoto K. T cell deficiency leads to liver carcinogenesis in Azoxymethane-treated rats. Experimental and Biological Medicine 2005; 231: 91-98.
Renugadevi J and Prabu SM. Cadmium-induced hepatotoxicity in rats and the protective effect of naringenin. Experimental
Toxicology and Pathology 2010; 62: 171-181
Dianzani M and Barrera G. (2008). Pathology and physiology of lipid peroxidation and its carbonyl products. In: Álvarez, S.
& Evelson, P. (ed.), Free Radical Pathophysiology, Transworld Research Network: Kerala, India. pp. 19-38.
Boveris A, Repetto MG, Bustamante J, Boveris AD and Valdez LB. (2008). The concept of oxidative stress in pathology.In: Álvarez, S.; Evelson, P. (ed.), Free Radical Pathophysiology, pp. 1-17, Transworld Research Network
Valko M, Rhodes CJ, Moncol J, Izakovic M and Mazur M. Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chemical and Biological Interaction 2006; 160: 1-40
Gibson BR, Smith JM, Lawrence SJ, Shelton N, Smith JM and Smart KA. Oxygen as toxin: oxidative stress and brewing yeast
physiology. Cerevisiae 2006; 31: 25-36.
Fridovich I. Superoxide radical and superoxide dismutases. Annual Review of Biochemistry1995; 64: 97–112.
Teixeira HD, Schumacher RI and Meneghini R. Lower Intracellular hydrogen peroxide levels in cells overexpressing CuZn-superoxide dismutase. Proceeding of National Academy of Science 1998; 95: 7872-5.
Klaassen CD, Liu J and Choudhuri S. Metallothionein: an intracellular protein to protect against cadmium toxicity. Annual Review of Pharmacological Toxicology 1999; 39: 267-294.
Ikediobi CO, Badisa VL, Ayuk-Takem LT, Laatinwo LM and West J. Response of antioxidant enzymes and redox metabolites to cadmium-induced oxidative stress in CRL-1439 normal rat liver cells. International Journal of Molecular biology 2004; 14: 87-92.
International Agency for Research on Cancer (IARC) monographs. Cadmium: IARC Press, Lyon 1993; 58:119-238.
Waisberg M, Joseph P, Hale B and Beyersmann D. Molecular and cellular mechanisms of cadmium carcinogenesis.
Toxicology 2003; 192: 95-117
Kawata K, Shimazaki R.and Okabe S. Comparision of gene expression profiles in HepG2 cells exposed to arsenic, cadmium, nickel, and three model carcinogens for investigating the mechanisms of metal carcinogenesis. Environmental and Molecular Mutagenesis 2009; 50: 46-59.
Chung AS and Maines MD. Differential effect of cadmium on GSH peroxidase activity in the Leydig and Sertoli cells of rat testis. Suppression by selenium and the possible relationship to heme concentration. Biochemical Pharmacology 1987; 36: 1367-1372.
Whiteside JR, Box CL, McMillan TJ and Allinson SL. Cadmium and copper inhibit both DNA repair activities of
polynucleotide kinase. DNA Repair 2010; 9: 83-89.
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