Ethanol extract of <i>Imperata cylindrica</i> Roots Potential as an Aphrodisiac in Male Wistar Rats http://www.doi.org/10.26538/tjnpr/v7i9.21

Main Article Content

I Nyoman G Wardana
I Gusti A Widianti
I Nyoman M Karmaya
Jaqueline Sudiman Muliani

Abstract

The roots of Imperata cylindrica are used by many ethnic groups in Indonesia as an aphrodisiac. This study determined the aphrodisiac properties of the ethanol extract of I. cylindrica roots in male Wistar rats. The experimental animal was 25 male and 15 female Wistar rats aged 4 – 5 months. The male rate had at least two previous copulation experiences. The ethanol extract of I. cylindrica was administered orally to the treatment group for 28 days at 100, 200, and 400 mg/kg. The group given sildenafil citrate (5 mg/kg BW) and distilled water served as positive and negative controls, respectively. On day 29, the serum testosterone level and sexual behavior parameters (mounting and intromission latency, mounting and intromission frequency, ejaculation latency, and postejaculatory mounting interval) were observed and assessed. Administration of the ethanol extract of I. cylindrica roots for 28 days resulted in decreased mounting and intromission latency and increased mounting and intromission frequency at all doses. There was an increase in ejaculation latency at 100 and 200 mg/kg BW doses and a decrease in the postejaculatory mounting interval at 100 and 200 mg/kg BW doses. A 100 mg/kg BW dose approached the giving sildenafil effect (5 mg/kg BW) as a positive control. Serum testosterone levels increased at all doses of the extract. The ethanol extract of I. cylindrica roots has the potential to act as an aphrodisiac, with the possible mechanism of action being an increase in testosterone levels. 

Article Details

How to Cite
Wardana, I. N. G., Widianti, I. G. A., Karmaya, I. N. M., & Muliani, J. S. (2023). Ethanol extract of <i>Imperata cylindrica</i> Roots Potential as an Aphrodisiac in Male Wistar Rats: http://www.doi.org/10.26538/tjnpr/v7i9.21. Tropical Journal of Natural Product Research (TJNPR), 7(9), 3989–3994. Retrieved from https://tjnpr.org/index.php/home/article/view/2625
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References

Anderson D, Laforge J, Ross MM, Vanlangendonck R, Hasoon J, Viswanath O, Kaye AD, Urits I. Male sexual dysfunction. Health Psychol Res. 2022; 10(3): 37533.

Irfan M, Hussain NHN, Noor NM, Mohamed M, Sidi H, Ismail SB. Epidemiology of male sexual dysfunction in Asian and European regions: A systematic review. Am J Mens Health. 2020; 14(4): 1-40.

Zhang H, Yip AW, Fan S, Yip PS. Sexual dysfunction among Chinese married men aged 30-60 years: A population-based study in Hong Kong. Urology. 2013; 81(2): 334-339.

Birowo P, Deswanto IA, Rasyid N. Epidemiology of erectile dysfunction: A cross-sectional web-based survey conducted in an Indonesian National Referral Hospital. F1000Res. 2019; 8: 1-12.

Lotti F, Maggi M. Sexual dysfunction and male infertility. Nat Rev Urol. 2018; 15(5): 287-307.

Cuenca AIC, Sampietro-Cresp A, Virseda-Chamorr M, Martín-Espinosa N. Psychological impact and sexual dysfunction in men with and without spinal cord injury. J Sex Med. 2015; 12(2): 436-444.

McBride JA, III CCC, Coward RM. Testosterone deficiency in the aging male. Ther Adv Urol. 2016; 8: 47-60.

Ahmed K, Hatzimouratidis K, Muneer A. Male sexual dysfunction and hypogonadism guidelines for the aging male. Eur Urol Focus. 2017; 3(4-5): 514-516.

Kovac JR, Rajanahally S, Smith RP, Coward RM, Lamb DJ, Lipshultz LI. Patient satisfaction with testosterone replacement therapies: The reasons behind the choices. J Sex Med. 2014; 11(2): 553-562.

Avasthi A, Grover S, Sathyanarayana-Rao TS. Clinical practice guidelines for management of sexual dysfunction. Indian J Psychiatry. 2017; 59(Suppl 1): S91-S115.

Barroso F, Ribeiro JC, Miranda EP. Phosphodiesterase type 5 inhibitors and visual side effects: A narrative review. J Ophthalmic Vis Res. 2021; 16(2): 248-259.

Corona G, Vignozzi L, Sforza A, Maggi M. Risks and benefits of late-onset hypogonadism treatment: An expert opinion. World J Mens Health. 2013; 31(2): 103-125.

da Cruz AC, Guerra NG, de Souza KEBP, de Castro Eleutério I, da Silva LC, Otoni EG, Alves MRA, Regis WCB. The action of herbal medicine on the libido: Aspects of nutritional intervention in increasing sexual desire. Nutrire. 2017; 42(1): 29.

Sin VJ-E, Anand GS, Koh H-L. Botanical medicine and natural products used for erectile dysfunction. Sex Med Rev. 2021; 9(4): 568-592.

Kotta S, Ansari SH, Ali J. Exploring scientifically proven herbal aphrodisiacs. Pharmacogn Rev. 2013; 7(13): 1-10.

Haryanti S, Widodo H, Fauzi F. Kajian tumbuhan obat yang banyak digunakan untuk aprodisiaka oleh beberapa etnis Indonesia [Study of medicinal plants that are widely used for aphrodisiacs by several Indonesian ethnic groups]. Media Litbangkes. 2019; 29(1): 51-64.

Fauzi F, Widodo H. Short communication: Aphrodisiac plants used by Dayak ethnic in Central Kalimantan province, Indonesia. Biodiversitas J Biol Div. 2019; 20(7): 1859-1865.

Singh R, Ali A, Jeyabalan G, Semwal A, Jaikishan. An overview of the current methodologies used for evaluation of aphrodisiac agents. J Acute Dis. 2013; 2(2): 85-91.

Lab B. Protocol booklet rat testosterone elisa kit cat.No: Ea0023ra 2023. [Online]. 2023. [cited 2023 Aug 23]. Available from: https://www.btlaboratory.com/index.php/Shop/Index/productShijiheDetail/p_id/3475.html.

Heijkoop R, Huijgens PT, Snoeren EMS. Assessment of sexual behavior in rats: The potentials and pitfalls. Behav Brain Res. 2018; 352: 70-80.

Ramalan MA, Shuaibu AB, Abdussalam US, Yaro AH. Aphrodisiac effect of methanol hypocotyls extracts ofBorassus aethiopum Mart. in male Wistar rats. Trop J Nat Prod Res. 2021; 5(10): 1852-1858.

Fouche G, Afolayan AJ, Wintola OA, Khorombi TE, Senabe J. Effect of the aqueous extract of the aerial parts of Monsonia angustifolia E. Mey. Ex A. Rich., on the sexual behavior of male Wistar rats. BMC Complement Altern Med. 2015; 15: 343.

Kenmogne H, Koloko B, Hambe C, Domkam J, Ngaha Njila M, Bend E, Oundoum Oundoum P, Massoma Lembè D, Dimo T. Effects of aqueous root extract of Carpolobia alba G. Don on sexual behavior in adult male rats. Andrologia. 2016; 48(8): 908-914.

Tang X, Olatunji OJ, Zhou Y, Hou X. In vitro and in vivo aphrodisiac properties of the seed extract from Allium tuberosum on corpus cavernosum smooth muscle relaxation and sexual behavior parameters in male Wistar rats. BMC Complement Altern Med. 2017; 17: 510.

Singh S, Nair V Fau - Gupta YK, Gupta YK. Evaluation of the aphrodisiac activity of Tribulus terrestris Linn. in sexually sluggish male albino rats. J Pharmacol Pharmacother. 2012; 3(1): 43-47

Erhabor JO, Idu M. 2017. Aphrodisiac potentials of the ethanol extract of Aloe barbadensis Mill. root in male Wistar rats. BMC Complement Altern Med. 2017; 17: 360-370.

Bianchi N, Prontera O, Dicuio M, Concetti S, Sforza A, Corona G. Male sex hormones in andrology today. In: Bettocchi C, Busetto GM, Carrieri G, Cormio L (Eds.). Practical clinical andrology. Italy: Springer; 2023. 251-261 p.

Corona G, Torres LO, Maggi M. Testosterone therapy: What we have learned from trials. J Sex Med. 2020; 17(3): 447-460.

Zirkin BR, Papadopoulos V. Leydig cells: Formation, function, and regulation. Biol Reprod. 2018; 99(1): 101-111.

Nwokike MO, Ghasi SI, Ogbonna AO, Anusiem CA. The effect of Imperata cylindrica root aqueous extracts on serum testosterone levels of hyperglycemic rats. J Pharma Pharma Sci. 2020; 4(1): 187.

da Cruz AC, Guerra NG, de Souza KEBP, de Castro Eleutério I, da Silva LC, Otoni EG, Alves MRA, Regis WCB. The action of herbal medicine on the libido: Aspects of nutritional intervention in increasing sexual desire. Nutrire. 2017; 42(1):42-49.

Jayalakshmi S, Patra A, Lal VK, Ghosh AK. Pharmacognostical standardization of roots of Imperata cylindrica Linn (Poaceae). J Pharm Sci Res. 2010; 2(8):472-6.

Khaerunnisa S, Aminah NS, Kristanti AN, Kuswarini S, Wungu CDK, Soetjipto S, Suhartati S. Isolation and identification of a flavonoid compound and in vivo lipidlowering properties of Imperata cylindrica. Biomed Rep. 2020;13(5):1-8.

Dhianawaty D, Ruslin R, Syamsunarno MRAA, Haminah H. Kandungan total flavonoid dari ekstrak metanol akar Imperata cylindrical (L) Beauv. (alang-alang) [Total flavonoid content of methanol extract of Imperata cylindrica (L) Beauv roots]. Talenta Conf Ser: TM. 2018;

(3): 25-28.