Phytochemical Screening, Antioxidant and Xanthine Oxidase Inhibitory Activities of <i>Vitis heyneana</i> Schult. Stem Extracts From Vietnam
http://www.doi.org/10.26538/tjnpr/v7i9.20
Keywords:
Xanthine oxidase, Phytoconstituents, Anti-gout, Antioxidant, Vitis heyneanaAbstract
Vitis heyneana Schult. (family Vitaceae) is locally known as ‗Wild Grapes‘. It has long been used traditionally as a remedy for irregular menstruation, furuncle, bronchitis, and arthritis-related diseases. Antioxidant and xanthine oxidase (XO) inhibitory activities have not been reported in this species. Therefore, the present study aim to investigate the antioxidant and XO inhibitory activities of the plant. Phytochemical screening of the ethanol stem extract was carried out using standard method. The antioxidant activity was evaluated using the 2,2-diphenyl-1-picryl hydrazyl (DPPH) radical scavenging method. The XO inhibitory activity was evaluated using standard procedure. Phytochemical screening showed the presence of carbohydrates, essential oils, amino acids, triterpenoids, saponins, coumarins, flavonoids, and tannins. The ethyl acetate fraction showed the highest antioxidant activity with IC50 value of 21.90 ± 0.17 µg/mL, followed by chloroform fraction (IC50 = 35.92 ± 0.28 µg/mL), petroleum ether fraction (IC50 = 52.22 ± 0.20 µg/mL), and aqueous fraction (IC50 = 143.37 ± 1.12 µg/mL). The plant extract exhibited significant XO inhibitory activity with the ethyl acetate fraction showing the highest activity (IC50 = 11.28 ± 0.41 µg/mL) followed by the aqueous fraction (IC50 = 17.51 ± 0.38 µg/mL), chloroform fraction (IC50
= 35.75 ± 0.19 µg/mL), and the petroleum ether fraction (IC50 = 130.20 ± 0.24 µg/mL). These results suggest the potential use of V. heyneana stems in the management of gout as well as an antioxidant to eliminate free radicals and reduce the oxidative stress associated with gout.
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