Phytochemical Profiling and Antioxidant Activities of Red Ginger (Zingiber officinale var. rubrum) Cultivated Eco-Farming http://www.doi.org/10.26538/tjnpr/v7i9.18

Main Article Content

Rahmat A.Hi. Wahid
Okti Purwaningsih
Puguh B. Pamungkas

Abstract

Red ginger (Zingiber officinale var. rubrum) is useful as an antioxidant, immunomodulator, antibacterial, and anti-inflammatory. Eco-enzymes can enhance ginger growth and increase phytochemical content and antioxidant activity. This study aimed to identify the composition of phytochemical compounds and antioxidant activity. The study was designed in a completely randomized design in 3 replications. This study consisted of 4 treatments: without eco-enzymes, 0.1% eco-enzymes, 0.3% eco-enzymes and 0.5% eco-enzymes. The phytochemical content of the ginger rhizome eight months after planting and its antioxidant activity were measured using various methods, such as the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method and the spectrophotometric method for measuring total flavonoid content (TFC) and total phenolic content (TPC). The study showed that ginger rhizome contained flavonoids, phenolic and terpenoid compounds in all treatments. Eco-enzymes significantly increased ginger rhizome's flavonoid and phenolic content (p<0.05). The results obtained are non-linear because the higher the concentration of eco-enzyme, both the flavonoid, phenolic, and antioxidant activity values are non-linear. The highest flavonoid content was obtained from ginger, with an eco-enzyme concentration of 0.3%, which was 0.11%, while the highest phenolic content was found at a concentration of 0.5%, 53.54%. In contrast, the percentage of inhibition in the DPPH test was 73.65%. Red ginger with 0.5% eco-enzyme has potent antioxidant activity with an IC50 value of 4.238 ppm. With the help of eco-enzymes, red ginger can be transformed into antioxidant-rich food products that are environmentally friendly. 

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How to Cite
Wahid, R. A., Purwaningsih, O., & Pamungkas, P. B. (2023). Phytochemical Profiling and Antioxidant Activities of Red Ginger (Zingiber officinale var. rubrum) Cultivated Eco-Farming: http://www.doi.org/10.26538/tjnpr/v7i9.18. Tropical Journal of Natural Product Research (TJNPR), 7(9), 3968-3973. https://tjnpr.org/index.php/home/article/view/2619
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How to Cite

Wahid, R. A., Purwaningsih, O., & Pamungkas, P. B. (2023). Phytochemical Profiling and Antioxidant Activities of Red Ginger (Zingiber officinale var. rubrum) Cultivated Eco-Farming: http://www.doi.org/10.26538/tjnpr/v7i9.18. Tropical Journal of Natural Product Research (TJNPR), 7(9), 3968-3973. https://tjnpr.org/index.php/home/article/view/2619

References

Oboh G, Akinyemi AJ, Ademiluyi AO. Antioxidant and inhibitory effect of red ginger (Zingiber officinale var. Rubra) and white ginger (Zingiber officinale Roscoe) on Fe 2+ induced lipid peroxidation in rat brain in vitro. ExpToxicol Pathol. 2012;64(1–2):31–6.

Ghasemzadeh A, Jaafar HZE, Rahmat A. Antioxidant activities, total phenolics and flavonoids content in two varieties of Malaysia young ginger (Zingiber officinale Roscoe). Molecules. 2010;15(6):4324–33.

Hosseinzadeh A, Bahrampour Juybari K, Fatemi MJ, Kamarul T, Bagheri A, Tekiyehmaroof N, Sharifi MA. Protective Effect of Ginger (Zingiber officinale Roscoe) Extract against Oxidative Stress and Mitochondrial Apoptosis Induced by Interleukin-1β in Cultured Chondrocytes. Cells Tissues Organs. 2017;204(5–6):241–250.

Aryanti PI, Haryanto J, Ulfiana E. Red Ginger (Zingiber officinale var. rubrum) massage reduces stiffness and functional Disability in Elderly with Osteoarthritis. J Ners. 2018;13(3):200–206.

Choi J, Lee J, Kim K, Choi HK, Lee SA, Lee HJ. Effects of Ginger Intake on Chemotherapy-Induced Nausea and Vomiting: A Systematic Review of Randomized Clinical Trials. Nutrients. 2022;14(23):4982.

Suciyati S, Sukrasno S, Kurniati NF, Adnyana IK. Antioxidant and anti-inflammatory activity of red ginger (Zingiber officinale Roscoe var. Sunti Val) essential oil distillation residues. Egyp J Chem. 2021;64(9):5031-5035.

Zhu J, Chen H, Song Z, Wang X, Sun Z. Effects of Ginger (Zingiber officinale Roscoe) on Type 2 Diabetes Mellitus and Components of the Metabolic Syndrome: A Systematic Review and Meta-Analysis of Randomized Controlled Trials. Evid Based Complement Alternat Med. 2018;9(2018):5692962.

Jafarzadeh A, Jafarzadeh S, Nemati M. Therapeutic potential of ginger against COVID-19: Is there enough evidence? J Tradit Chinese Med Sci. 2021;8(4):267–79.

Wijaya RM, Hafidzhah MA, Kharisma VD, Ansori ANM, Parikesit AA. COVID-19 in silico drug with Zingiber officinale natural product compound library targeting the Mpro protein. Makara J Sci. 2021;25(3):162-171.

Lorensia A, Pratama AM, Sukarno DA, Suryadinata RV. Effects of Red Ginger (Zingiber officinale var rubrum)to Improve Lung Function in reducing the risk of COVID-19 in Stable COPD Patients. Tei Med J.2021;44(6):2667-2676.

Mao Q, Xu XY, Cao S, Gan RY, Corke H, Beta T, Li HB. Bioactive compounds and bioactivities of ginger (Zingiber officinale Roscoe). Foods. 2019;8(6):185.

Kamal GM, Nazi N, Sabir A, Saqib M, Zhang X, Jiang B, Khan J, Noreen A, Uddin J, Murtaza S. Yield and Chemical Composition of Ginger Essential Oils as Affected by InterVarietal Variation and Drying Treatments of Rhizome. Separations. 2023; 10(3):186.

Purwaningsih O, Pamungkas B, Wahid R. Study on the Use of Eco-Enzymes to Improve Rhizome Quality and Phytochemical Content of Red Ginger (Zingiber Officinale Var.Rubrum). Exc Res Report, Universitas PGRI Yogyakarta. 2021.

Akanmu AO, Olowe OM, Phiri AT, Nirere D, Odebode AJ, Umuhoza NJK, Asemoloye MD, Babalola OO. Bioresources in Organic Farming: Implications for Sustainable Agricultural Systems. Horticulturae. 2023;9(6):659.

Vedashree M, Asha MR, Roopavati C, Naidu MM. Characterization of volatile components from ginger plant at maturity and its value addition to ice cream. J Food Sci Technol. 2020;57(9):3371–3380.

Purwaningsih O, Sumarmi S, Nonsi M. Growth Response and Production of Baby Cucumber (Cucumis Sativus L) In Various Eco-Enzyme Applications and Plant GrowthPromoting Rhizobacteria. J Pen Per Ter.2021;23(2):245–253.

Government of Kulon Progo Regency. Geographically of Kulon Progo Regency [Internet]. 2023. Available from: https://kulonprogokab.go.id/v31/detil/7670/geografis.

Nguyen ST, Vo PH, Nguyen TD, Do NM, Le BH, Dinh DT,Truong K, Pham P. Ethanol extract of Ginger (Zingiber officinale Roscoe) by Soxhlet method induces apoptosis in human hepatocellular carcinoma cell line. Biomed Res Ther. 2019;6(11):3433–3442.

Ali AMA, El-Nour MEAM, Yagi SM. Total phenolic and flavonoid contents and antioxidant activity of ginger (Zingiber officinale Rosc.) rhizome, callus and callus treated with some elicitors. J Genet Eng Biotechnol. 2018;16(2):677–682.

Yan-Hwa C, Chang CL, Hsu HF. Flavonoid content of several vegetables and their antioxidant activity. J. Sci. Food Agric.2000;80(5):561-566.

Deka H, Choudhury A, Dey BK. An Overview on Plant Derived Phenolic Compounds and Their Role in Treatment and Management of Diabetes. J Pharmacopuncture. 2022;25(3):199–208.

Reis A, Rocha S, Dias IH, Costa R, Soares R, SánchezQuesada JL, Perez A, de Freitas V. Type 2 Diabetes mellitus alters the cargo of poly)phenol metabolome and the oxidative status in circulating lipoproteins. Redox Biol. 2023;59: 102572:1-10.

Diakos A, Silva ML, Brito J, Moncada M, de Mesquita MF, Bernardo MA. The Effect of Ginger (Zingiber officinale Roscoe) Aqueous Extract on Postprandial Glycemia in Nondiabetic Adults: A Randomized Controlled Trial. Foods. 2023;12(5):1037.

Zhang S, Kou X, Zhao H, Mak KK, Balijepalli MK, Pichika MR. Zingiber officinale var. rubrum: Red Ginger’s Medicinal Uses. Molecules. 2022;27(3):775.

Zhou X, Münch G, Wohlmuth H, Afzal S, Kao M-H, AlKhazaleh A, Low M, Leach D and Li CG. Synergistic inhibition of pro-inflammatory pathways by ginger and turmeric extracts in RAW 264.7 cells. Front. Pharmacol. 2022;9(13):818166.

Egharevba E, Chukwuemeke-Nwani P, Eboh U, Okoye E, Bolanle IO, Oseghale IO, Imieje VO, Erharuyi O, Falodun A. Evaluation of the antioxidant and hypoglycaemic potentials of the leaf extracts of Stachytarphyta jamaicensis(Verbenaceae). Trop J Nat Prod Res. 2019;3(5):170–174.

Singh S, Bansal A, Singh V, Chopra T, Poddar J. Flavonoids, alkaloids and terpenoids: a new hope for the treatment of diabetes mellitus. J Diabetes Metab Disord. 2022;21(1):941–950.

Mot MD, Gavrilaș S, Lupitu AI, Moisa C, Chambre D, Tit DM, Bogdan MA, Bodescu AM, Copolovici L, Copolovici DM, Bungau SG. Salvia officinalis L. Essential Oil: Characterization, Antioxidant Properties, and the Effects of Aromatherapy in Adult Patients. Antioxidants (Basel).

;11(5):808.

Wang C, Liu X, Lian C, Ke J, Liu J. Triterpenes and Aromatic Meroterpenoids with Antioxidant Activity andNeuroprotective Effects from Ganoderma lucidum. Molecules. 2019;24(23):4353.

Cai C, Ma J, Han C, Jin Y, Zhao G, He X. Extraction and antioxidant activity of total triterpenoids in the mycelium of a medicinal fungus, Sanghuangporus sanghuang. Sci Rep. 2019;9(1):1–10.

Ghasemzadeh A, Jaafar HZE, Rahmat A. Variation of the Phytochemical Constituents and Antioxidant Activities of Zingiber officinale var. rubrum Theilade Associated with Different Drying Methods and Polyphenol Oxidase Activity. Molecules. 2016;21(6).1-12.

Fadlilla T, Budiastuti MtS, Rosariastuti MR. Potential of Fruit and Vegetable Waste as Eco-enzyme Fertilizer for Plants. J Penelit Pendidik IPA. 2023;9(4):2191–200.

Ali AMA, El-Nour MEAM, Yagi SM. Total phenolic and flavonoid contents and antioxidant activity of ginger (Zingiber officinale Rosc.) rhizome, callus and callus treated with some elicitors. J Genet Eng Biotechnol. 2018;16(2):677–682.

Braakhuis A. Evidence on the Health Benefits of Supplemental Propolis. Nutrients. 2019;11(11):1-15.

Lesjak M, Srai SKS. Role of Dietary Flavonoids in Iron Homeostasis. Pharmaceuticals. 2019;12(3):1-21.

Mutha RE, Tatiya AU, Surana SJ. Flavonoids as natural phenolic compounds and their role in therapeutics: An overview. Future J. Pharm. Sci. 2021;7(1): 25.

Bayele HK, Balesaria S, Srai SKS. Phytoestrogens modulate hepcidin expression by Nrf2: Implications for dietary control of iron absorption. Free Radic Biol Med. 2015;1(89):1192–202.

Herawati IE, Saptarini NM. Phytochemical studies on red ginger (Zingiber officinale Roscoe var. Sunti Val). Maj Farmasetika. 2019;4(0):22–27.

Rudrapal M, Khairnar SJ, Khan J, Dukhyil AB, Ansari MA, Alomary MN, Alshabrmi FM, Palai S, Deb PK, Devi R.Dietary Polyphenols and Their Role in Oxidative StressInduced Human Diseases: Insights Into Protective Effects, Antioxidant Potentials and Mechanism(s) of Action. Front

Pharmacol. 2022;13:1–15.

Kruk J, Aboul-Enein BH, Duchnik E, Marchlewicz M. Antioxidative properties of phenolic compounds and their effect on oxidative stress induced by severe physical exercise. J Physiol Sci. 2022;72(1):1–24.

Asamenew G, Kim HW, Lee MK, Lee SH, Kim YJ, Cha YS, Yoo SM, Kim JB. Characterization of phenolic compounds from normal ginger (Zingiber officinale Rosc.) and black ginger (Kaempferia parviflora Wall.) using UPLC–DAD–QToF–MS. Eur. Food Res. Technol. 2018; 1(245):653-665.

Amalia RT, Sabila FI. Phytochemical Screening and Total Phenolic CompoAmalia, Rizka Tazky und Fauzia Indah Sabila. 2021. Phytochemical Screening and Total Phenolic Compounds of Red Ginger (Zingiber officinale) and Secang Wood (Caesalpinia sappan) As Preliminary Test of Antiart. Chim Nat Acta. 2021;9(1):14–19.