LC-MS Based Phytochemical Perspective, ACE Inhibition Potential and Pharmacokinetics Study of Humulus lupulus Flower Extract http://www.doi.org/10.26538/tjnpr/v7i9.16
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Abstract
The growing burden of cardiovascular diseases (CV) has been a major cause of concern for the world, which has further aggravated during the Covid-19 pandemic, due to post-SARS-CoV-2 mediated complications among recovered patients. The quest to explore new, and more efficacious, safe alternative therapeutic products for cardiovascular diseases and their associated symptoms have been the thrust of scientific importance. Angiotensin-converting enzyme (ACE) plays a pivotal role in hypertension, a disease condition associated with cardiovascular diseases. In the present study, Humulus lupulus was extracted in different solvents and quantified for total flavonoid, phenolic and ACE inhibition activity estimated using aluminium chloride, Folin Ciocalteu reagent, and Cushman and Cheung methods respectively. Further, liquid chromatography mass spectrometry (LC-MS) profiling of the phytocompound followed by Pharmacokinetics, drug-likeness behavior, and toxicity prediction using SWISS-ADME and Protox-II were analyzed. Results revealed the mixture to be rich in sphingolipids, polyphenolics, terpenes, flavonoids, and others. The study suggests the synergistic role of the compounds on ACE inhibition activity shown by the extract. However, the study needs to be further extended to screen the responsible compound for the inhibition activity and mechanism of action. The evaluation of these compounds for their pharmacokinetics properties opens up avenues to explore new molecules for the purpose of drug designing.
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