Extraction, Characterization and Larvicidal Activity of Essential Oil and Hydrosol from Sida acuta Burm. f. Leaves Grown in Nigeria

doi.org/10.26538/tjnpr/v5i1.29

Authors

  • Isaac S. Njoku Department of Chemistry, Faculty of Science, University of Lagos, Akoka-Yaba, Lagos, Nigeria
  • Maurene U. Ichide Department of Chemistry, Faculty of Science, University of Lagos, Akoka-Yaba, Lagos, Nigeria
  • Nisar-Ur Rahman Department of Pharmacy,COMSATS University Islamabad, Abbottabad Campus, Pakistan.
  • Muhammad Ahsan Khan Department of Pharmacy,COMSATS University Islamabad, Abbottabad Campus, Pakistan.
  • Ngozi A. Chibuko Department of Marine Science, Faculty of Science, University of Lagos, Akoka-Yaba, Lagos, Nigeria
  • Olayinka T. Asekun Department of Chemistry, Faculty of Science, University of Lagos, Akoka-Yaba, Lagos, Nigeria
  • Oluwole B. Familoni Department of Chemistry, Faculty of Science, University of Lagos, Akoka-Yaba, Lagos, Nigeria

Keywords:

Hydrodistillation, Larvicidal, Sida acuta, Volatile oil, Hydrosol

Abstract

Mosquitoes transmit the malaria parasite, causing diseases and thousands of deaths annually. The major problems associated with the use of chemicals for the control of mosquitoes include the development of resistance to these chemicals by the plasmodium parasite and their undesirable toxic effects on humans and the environment. In this research, the volatile oil was extracted (in n-hexane) from the air-dried leaves of Sida acuta by hydrodistillation method. The hydrosol was extracted with n-hexane in the ratio 10:2. The oils were analysed using Gas Chromatography–Mass Spectrometry (GC-MS).The larvae of the mosquito species were exposed to five different test concentrations of the volatile oil and hydrosol oil of Sida acuta and were assayed. The larval mortality was observed and IC50 value was calculated using the probit analysis test. The GC–MS chromatograph of the oil revealed a total of 17 volatile constituents. Heneicosane (13.73%), docosane (11.11%), 1-iodohexadecane (10.06%) and hexa-hydrofarnesyl acetone (9.55 %) were the predominant compounds. The hydrosol oil comprised of 11 volatile constituents with triacontane (16.76 %), phytane (16.33 %), tricosane (14.91%) and octane (13.04 %) as the most abundant major constituents. The oils displayed varying degrees of larvicidal activities against the larvae, with highest larval mortality percentage of 80 % and 70% at 500 mg/L respectively in comparison to chloroquine, positive control (90%) and hexane, negative control (0%). The results show that the essential oil and hydrosol of Sida acuta Burm possesses strong larvicidal properties. 

Author Biography

Isaac S. Njoku, Department of Chemistry, Faculty of Science, University of Lagos, Akoka-Yaba, Lagos, Nigeria

Department of Pharmacy,COMSATS University Islamabad, Abbottabad Campus, Pakistan.

 

References

Mann A, Gbate M, Umar AN. Sida acuta subspecie acuta, Medicinal and economic plant of Nupeland, Nigeria. Jube Evans Boooks and Publication; 2003. 241p.

Karou SD, Nadembega W, Ilboudo DP, Ouerm D., Gbeassor M, De Souza C, Simpore J. Sida acuta Burm. F. A medicinal plant with numerous potencies. Afri J Biotechnol. 2007;6(25):2953-2959.

Karou D, Savadogo A, Canini A, Yameogo S, Montesano C, Simpore J, Colizzi V, Traore AS. Antibacterial activity of alkaloids from Sida acuta. Afri. J. Biotechnol. 2006; 5(2):195-200.

Abat JK, Kumar S, Mohanty A. Ethnomedicinal, phytochemical and ethnopharmacological aspects of four medicinal plants of Malvaceae used in Indian traditional medicines: a review. Med. 2017; 4(4):75.

Ezeabara CA and Egenti MO. Phytochemical and antimicrobial investigations on various parts of Sida acuta Burm. f. J. Ayurvedic Herb. Med. 2018; 4(2):71-75.

Tcheghebe OT, Seukep AJ, Tatong FN. Ethnomedicinal uses, phytochemical and pharmacological profiles, and toxicity of Sida acuta Burm. f.: A review article. The Pharma Innovation. 2017; 6(6, Part A):1.

Adesina DA, Adefolalu SF, Jigam AA, Lawal B. Antiplasmodial effect and sub-acute toxicity of alkaloid, flavonoid and phenolic extracts of Sida acuta leaf on Plasmodium berghei-infected animals. J Taibah Univ Sci. 2020; 14(1):943-953.

Zakariyya Y, Adefolalu F, Abubakar A. Antiplasmodial Effects of Crude Ethanol and Alkaloidal Extracts of Sida acuta Leaf in Mice. J Sci Technol Math Edu 2019; 15(1):268-281

Njoku IS, Asekun OT, Familoni OB, Abiodun E. The Effect of Post-Harvest Drying Methods on the Chemical Composition and yield of the Essential Oils of Secamone afzelii (Asclepiadaceae) grown in Badagry, Lagos State, Nigeria. J Chem Soc. Nigeria.2019; 44(1): 088-095.

Njoku IO, Asekun, OT, Familoni OB. Effects of different drying methods on the chemical composition of the essential oils of Ocimum gratissimum L. Leaves. Nig J Pure Appl Sci.2017; 3(30):3109-3115.

El-Sayed AM. The Pherobase: Database of Insect Pheromones and Semiochemicals (2012). http://www.pherobase.com.

http://webbook.nist.gov/chemistry/ (accessed 22 January 2020).

Kamaraj C, Bagavan A, Elango G, Zahir AA, Rajakumar G, Marimuthu, S, Rahuman A. A. Larvicidal activity of medicinal plant extracts against Anopheles subpictus & Culex tritaeniorhynchus. Indian J Med Res. 2011; 134(1):101.

Tabanca N, Ali Z, Bernier UR, Epsky N, Nalbantsoy A, Khan IA, Ali A. Bioassay-guided isolation and identification of Aedes aegypti larvicidal and biting deterrent compounds from Veratrum lobelianum. Open Chem. 2018; 16(1):324-332.

Ojekale AB, Lawal OA, Adeola AS, Folorunso OS, Azeez II, Opoku AR. Volatile constituents, antioxidant and insecticidal activities of essential oil from the leaves of Thaumatococcus danielli (Benn.) Benth. from Nigeria. IOSR J Pharm. 2013;3(3):1-5.

Cespedes CL, Molina SC, Munoz E, Lamilia C, Alarcon J, Palacios SM, Carpinella MC, Avila JG. The insecticidal, molting disruption and insect growth inhibitory activity of extracts from Condalia microphylla Cav. (Rhamnaceae), Ind. Crops Prod. 2013; 42:78-86.

El-Harwary SS, El-Tantawy ME, Rabel MA, Badr WK. Chemical composition and biological activities of essential oils of Acadirachta indica A. Juss, Int J Appl Res Nat Prod.2013; 6(4):33-42.

Mediouni JJB. Essential oil as a source of bioactive constituents for insect pests of economic importance in Tunisia. J Med Arom Plants. 2014; 3(2):1-7.

Melnikov NN. Chemistry of pesticides, Springer Science & Business Media; 2012 Dec 6; 26 p.

Nurtazina AN, Halmenova ZB, Umbetova AK, Buresheva GS, Aisa HA. Lipophilic components of Satureja amani. News of The National Academy of Sciences of the Republic of Kazakhstan-Series Chemistry and Technology. 2017: 1(2):12- 17.

Mohammed SI, Vishwakarma KS, Maheshwari V. Evaluation of larvicidal activity of essential oil from the leaves of Coccinia grandis against three mosquito species. J Arthropod Borne Dis. 2017; 11(2):226-235.

Pandey SK, Upadhyay S, Tripathi Ak. Insecticidal and repellant activities of thymol from the essential oil of Trachyspermum ammi (Linn) Sprague seeds against Anopheles stephensi, J Parasitol Res. 2009; 105(2):507-512.

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Published

2021-01-01

How to Cite

S. Njoku, I., U. Ichide, M., Rahman, N.-U., Ahsan Khan, M., A. Chibuko, N., T. Asekun, O., & B. Familoni, O. (2021). Extraction, Characterization and Larvicidal Activity of Essential Oil and Hydrosol from Sida acuta Burm. f. Leaves Grown in Nigeria: doi.org/10.26538/tjnpr/v5i1.29. Tropical Journal of Natural Product Research (TJNPR), 5(1), 211–216. Retrieved from https://tjnpr.org/index.php/home/article/view/251