Restorative Potentials of Aqueous Telfairia occidentalis Seeds Extract on the Hippocampal Nissl Granules and Short-Term Memory in Scopolamine Hydrobromide-Induced Alzheimer’s Type Cognitive Dysfunction Rats doi.org/10.26538/tjnpr/v5i1.24
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Abstract
Cognitive dysfunction is one of the major health problems including Alzheimer’s that is debilitating in nature. Neuroprotections are strategies and relative mechanisms that are able to defend the central nervous system against neuronal injury due to acute or chronic neurodegenerative disorders. This study elucidated the potentials of aqueous Telfairia occidentalis seed extract on Nissl substance in the hippocampus and short-term memory using scopolamine-induced Alzheimer’s type cognitive dysfunction rats. With ethical approval from the Faculty of Basic Medical Sciences, University of Calabar, Calabar, Nigeria (FAREC-FBMS 042ANA3719), thirty Wistar rats weighing between 180-200 g were used for the study and grouped into five; A, B, C, D and E. Alzheimer’s type cognitive dysfunction was induced in groups B through E before the extract and drug administration, followed by the novel object recognition test and histochemical tissue processing for Nissl granules demonstration with Cresyl Fast Violet stain. Neurobehavioral results revealed enhancement of short-term memory in the treated groups. The Nissl body stained with Cresyl Fast Violet under light microscope revealed less stain in group B. Groups C and E were mildly stained while Group D was deeply stained. The deeply stained Nissl granules in group D indicates increased protein synthesis which may cause proliferation of synapses in the hippocampal pyramidal cells hence, leading to enhanced learning and memory. In conclusion, aqueous extract of Telfairia occidentalis restored depleted hippocampal Nissl granules and enhanced short-term memory in scopolamine hydrobromide-induced Alzheimer’s type cognitive dysfunction rats.
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Weiss RF and Fintelmann V. Herbal medicine (2nd ed.) New York. 2000; 13-16 p.
Lobo V, Patil A, Phatak A, Chandra N. Free radicals, antioxidants and functional foods: Impact on human health. Pharm Rev. 2010; 4:118-126.
Salman TM, Olayiki LA, Oyeyemi WA. Effects of the aqueous extracts of Telfairia occidentalis on some haematological reproductive indices and blood glucose. Afr J Biotechnol. 2008; 7:299-303.
Moyo M, Amoo SO, Ncube B, Ndhlala AR, Finnie JF, Van Stadem J. Phytochemical and antioxidant properties of unconventional leafy vegetables consumed in southern Africa. South Afri J Bot. 2013; 84:65-71.
Liang D, Zhou Q, Gong W, Wang Y, Nie Z, He H, Li, J, Wu, J., Wu, C, Zhang, J. Studies on the antioxidant and hepatoprotective activities of polysaccharides from Talinum triangulare. J Ethnopharm. 2011; 136:316-321.
Andarwulan N, Batari R, Sandrasari DA, Bolling B, Wijaya H. Flavonoid content and antioxidant activity of vegetables from Indonesia. Food Chem. 2010; 121:1231-1235.
Oboha G, Ademilayia AO, Akinyemia AJ, Henleb T, Jamiyu A, Saliua JA, Uwe SU. Inhibitory effects of polyphenol-rich extracts of jute leaf (Corchorus olitorius) on key enzyme linked to type 2 diabetes (α-amylase and α-glucosidase) and hypertension (angiotensin 1 converting) in vitro. J Funct food. 2012; 4:456-458.
McCarty MF. Scavenging of peroxinitrite-derived radicals by flavonoids may support endothelial NO synthase activity, contributing to the vascular protection associated with high fruit and vegetable intakes. Med Hypo. 2008; 70:170-181.
Packiavathy IASV, Agilandeswari P, Musthafa KS, Randian SK, Ravi AV. Antibiofilm and quorum sensing inhibitory potential of Cuminum cyminum and its secondary metabolites methyl eugenol against Gram negative bacterial pathogens. Food Res Int. 2012; 45:85-92.
Kim J, Jayaprasha GK, Uckoo RM, Patil BS. Evaluation of chemopreventive and cytotoxic effect of lemon seed extracts on human breast cancer (MCF-7) cells. Food Chem Toxicol. 2012; 50:423-430.
Pribis P and Shukitt-Hale B. Cognition: the new frontier for nuts and berries. The Am J Clin Nutr. 2014; 100:347S-352S.
Ye X, Bhupathiraju SN, Tucker KL. Variety in fruit and vegetable intake and cognitive function in middle-aged and plder Puerto Rican adults. Br J Nutr. 2013; 109(3):503-510.
Spencer JP. The impact of fruit flavonoids on memory and cognition. Bri J Nutr. 2010; 104(S3):S40-S47.
Shukitt-Hale B, Cheng V, Joseph JA. Effects of blackberries on motor and cognitive function in aged rats. Nutr Neurol. 2009; 12(3):135-140.
Tabassum N, Rasool S, Malik ZA, Ahmad F. Natural Cognitive Enhancers. J Pharm Res. 2012; 5(1):153-160.
Pharm-Huy LA, He H, Phar-Huy C. Free radical. Antioxidants in disease and health. Int J Biomed Sci. 2008; 4(2):89-96.
Zhang L, Ravipati AS, Koyyalamudi SR, Jeong SC, Reddy N, Smith PT, Barlett J, Shanmugam K, Munch G, Wu MJ. Antioxidant and antiiflammatory activities of selected medicinal plants containing phenolic and flavonoid compouds. J Agric Food Chem. 2011; 59(23):12361-12367.
Manach C, Scalbert A, Morand C, Remesy C Jimenez L. Polyphenols: food sources and bioavailability. The Am J Clin Nutr. 2004; 79(5):727-747.
Nakiae SN, Rade D, Kevin D, Strucelj D, Mokrove AK, Bartoliae M. Chemical characteristics of oils from naked and husk seeds of Cucurbita pepo L. Euro J Lip Sci Technol. 2006; 108:936-943.
Bombardelli E and Morazzoni P. Cucurbita pepo L. Fitoterapia. 1997; 68:291-302.
Nkosi CZ, Okpoku AR, Terbalanche SE. Antioxidative effects of pumpkin seed (Cucurbita pepo) protein isolate in CCl4-Induced liver injury in low-protein fed rats. PhytotherRes. 2006; 20(11):935-940.
Lorke D. A new approach to practical acute toxicity testing. Arch Toxicol. 1983; 54(4):275-287.
Goulart BK, de Lima, MNM, de Farias CB, Reolon GK, Almeida VR, Quevedo J, Kapczinski F, Schroder N, Roesler R. Ketamine impairs recognition memory consolidation and prevents learning-induced increase in hippocampal brain-derived neutrotrophic factor levels.
Neurosci. 2010; 167:969-973.
Silvers JM, Harrod SB, Mactutus CF, Booze RM. Automation of the novel object recognition task for use in adolescent rats. J Neurol Metab. 2007; 166:99-103.
Leger M, Quiedeville A, Bouet V, Haelewy B, Boulouard M, Scumann-Bard P, Fteret T. Object recognition test in mice. Nat Prod. 8(12):2531-2537.
Eru EM, Paulinus SO, Igiri AO, Akpaso MI. Enhanced Effect of Aqueous Extract of Telfairia occidentalis Seed on the Microstructure of the Hippocampus of Scopolamine Hydrobromide-Induced Cognitive Dysfunction Rats, Asian J Res Rep Neu. 2020; 3(1):5-10.
Eru EM, Paulinus SO, Udoh-Affah GU, Uruakpa KC, Oku ME, Edet UI, Igiri AO. Neurobehavioural Enhancement in Scopolamine Hydrobromide-Induced Alzheimer Type Cognitive Dysfunction in Rats Following Administration of Ethanol Seed Extract of Telfairia occidentalis (Hook.f.) Cucurbitaceae. Trop J Nat Prod Res. 2020; 4(7):282-285.
Mugwagwa AT, Gadaga LL, Pote W, Tagwireyi D.Antiamnesic effects of hydrethanolic extract of Crinmum macowanii on scopolamine-induced memory impairment in mice. J Neu Dis. 2015; 24250-24259.http://dx.doi.org/10.11.55/2015/242505.
Ekong MB, Igiri AO, Salami E, Egwu OA. Effects of artesunate on Nissl bodies of the cerebellum of wistar rats. J Exp Clin Ana. 2008; 7(1):13-16.
Davis RL and Robertson DM. Textbook of neuropathology (2nd ed.). Williams and Wilkins, London. 1991; 23-26pp.
JPND Research. What is neurodegeneration? Available at www.neurodegenerationresearch.eu. Retrieved February 7, 2015.
Owoeye O and Gabriel MO. Protective Effects of Aqueous Extract of Telfairia occidentalis on Mercury-Induced Histological and Oxidative Changes in the Rat. Hippocampus and Cerebellum. Afr J Biomed Res. 2016; 19:41- 247.
Anani SE, Eru EM, Okon DE, Uruakpa KC, Ugbem TI, Igiri AO. The Effect of Averrhoa Carambola (Star Fruit) Aqueous Fruit Extract on the Hippocampal Astrocyte Expression following Diazepam-Induced Neurotoxicity in Wistar Rats. Trop J Nat Prod Res. 2020; 4(12):1170-1173.