Hepatoprotective Effect of Andropogon gayanus against Paracetamol- and Carbon Tetrachloride-Induced Liver Toxicity in Rats
doi.org/10.26538/tjnpr/v5i1.25
Keywords:
Andropogon gayanus, Carbon tetrachloride, Paracetamol, Oxidative stress, Liver, ToxicologyAbstract
Andropogon gayanus has found use in tropical Africa for its vast medicinal properties including liver healing potentials. This study nvestigated the efficacy of Andropogon gayanus extract in paracetamol- and carbon tetrachloride-mediated hepatic injury in rats. Two models were employed to study the toxicity. The first was with 3 g/kg of paracetamol orally on day four and secondly with 1 mL/kg carbon tetrachloride intraperitoneally (1:1 in olive oil) on the third and fourth day, respectively. The efficacy of the extract was tested using varying doses of 200, 400 and 800 mg/kg, respectively for five days. After drug treatments, animals were subjected to humane death while blood sample was taken for determination of liver function markers [(alanine aminotransferase (ALT), aspartate aminotransferase (AST), alanine phosphatase (ALP), and bilirubin) with level of oxidative stress by assessing (malondialdehyde (MDA), catalase (CAT), gluthathione (GSH) and superoxide dismutase (SOD)] while the liver was excised for histological studies. With an estimated LD50 value of greater than 5000 mg/kg, the extract is said not to be toxic. Phytochemistry showed that tannins, triterpenes, saponins, flavonoids, glycosides and alkaloids were present. Pre-treatment with extract at 400 mg/kg as well as 800 mg/kg showed significant (p ≤ 0.05) reduction in all serum liver markers with significant (p ≤ 0.05) amelioration of oxidative stress when compared to the toxicity control. Results from this research could indicate that methanol extract of Andropogon gayanus possesses antioxidant and hepatoprotective potentials and may be beneficial for the treatment of liver diseases.
References
Kandimalla R, Kalita S, Saikia B, Choudhury B, Singh YP, Kalita K, Dash S, Kotoky J. Antioxidant and hepatoprotective potentiality of Randia dumetorum Lam. leaf and bark via inhibition of oxidative stress and inflammatory cytokines. Front Pharmacol. 2016; 7:1-8.
Hall JE and Guyton AC. Guyton and Hall text book of medical physiology. Philadelphia. 2016. 877 p.
Hoffman RS, Burns MM, Gosselin S. Ingestion of caustic substances. N Eng J Med. 2020; 382:1739-1748.
Fuster D and Samet JH. Alcohol use in patients with chronic liver disease. N Eng J Med. 2018; 379(13):1251-1261.
Cheema E, Al-Aryan A, Al-hamid A. Medicine use and medicine-related problems in patients with liver cirrhosis: a systematic review of quantitative and qualitative studies. Eur J Clin Pharmacol. 2019; 75:1047-1058.
Mohammed N, Yaro AH, Nazifi AB. Evaluation of hepatoprotective activity of methanol stem bark extract of Haematostaphis barteri Hook. F. against paracetamol and carbon tetrachloride-induced liver injury in rats. Afr J Pharmacol Ther. 2017. 6(2):88-95.
Mohammed N, Yaro AH, Nazifi AB. Bombax costatum Pellegr. and Vuillet stem bark extract prevents paracetamol and carbon tetrachloride-induced liver injury in rats. Trop J Nat Prod Res. 2018; 2(5):220-226.
Okwori AI and Aken’Ova ME. Nutritive value assessment of some Gamba grass (Andropogon gayanus) accessions in the Southern Guinea Savannah of Benue State, Nigeria. Am J Agric Sci. 2017; 4(5):99-106.
Etuk EU, Ugwah MO, Ajagbonna OP, Onyeyili PA. Ethnobotanical survey and preliminary evaluation of medicinal plants with antidiarrhoea properties in Sokoto State, Nigeria. J Med Plants Res. 2009; 3(10):763-766.
Malgras D. Arbres et arbustes guérisseurs des savanes maliennes. Editions Karthala, 22 - 24, boulevard Arago, 75013 Paris, 1992. 480 p.
Saadou M. Les plantes médicinales du Niger: premier supplément à l'enquête ethnobotanique de. Rev Méd PharmAfr. 1979; 3(7):11-24.
Aliyu MA, Abdullahi AA, Ugya AY. Toxicity screening of selected Poaceace species in Kano, Northern Nigeria. World J Pharm Med Res. 2017; 3(4):135-139.
Zandam SU, Nazifi AB, Odoma S, Zezi AU. Antinociceptive and anti-inflammatory activities of methanol root extract of Andropogon gayanus Kunth (Poaceace) in rodents. Afr J Pharmacol Ther. 2020; 9(1):27-33.
Evans WC. Trease and Evans Pharmacognosy, 15th edn. W.R Saunders, London. 2002. 233-336 p.
Lorke D. A new approach to practical acute toxicity testing. Arch Toxicol. 1983; 54:275-287.
Oraebosi MI, Good GM, Chia T, Oyeniran OI. Bombax costatum extract abrogates piroxicam-mediated hepatic and gastric toxicities in rats. Ann Pharm Fr. 2020; 78:507-514.
Oraebosi MI, Olurishe TO, Ayanwuyi LO. Chronomodulated Nifedipine Offers Reno-protection in Glimepiride Treated Hyperglycaemic Rats.” J Pharm SciTechnol. 2016; 6(1):40-44.
Arthur SJ and John B. A colour Atlas of Histopathological Staining Techniques. Wolf MED. 1978. 14-20 p.
Cassarette I, Klaassen CD, Amdur MO. Doulls J. Principles of Toxicology In: Cassarett and Doul’s Pharmacology, The Basic Science of Poison Edited by Curtis, D. Klaassen, 5thedition copyright McGraw – Hill (USA) Health Professional Division New York. 1996. 13-33; 403-414 p.
Lala V, Goyal A, Bansal P, Minter DA. Liver function tests. [Updated July 2020]. In: StatPearls [Internet]. Treasure Island (FL). 2020.
Hayashi PH. Drug-Induced Liver Injury Network Causality Assessment: Criteria and Experience in the United States. Int J Mol Sci. 2016; 17:201.
Du K, Ramachandran A, Weemhoff JL, Chavan H, Xie Y, Krishnamurthy P, Jaeschke H. Editor's Highlight: Metformin Protects Against Acetaminophen Hepatotoxicity by Attenuation of Mitochondrial Oxidant Stress and Dysfunction. Toxicol Sci. 2016; 154:214-226.
Du K, Ramachandran A, Jaeschke H. Oxidative stress during acetaminophen hepatotoxicity: Sources, pathophysiological role and therapeutic potential. Redox Biol. 2016; 10:148-156.
Laurence LB, Keith LP, Donald KB, Lain LOB. Goodman and Gilman’s Manual of Pharmacology and Therapeutics. New York. 2008. 96-98 p.
Vishwanath J, Preetham GB, Anil KB, Satwik RJ. A Review on Hepatoprotective Plants. Int J Drug Dev Res. 2012; 4(3):1-8.
Kensa VM and Yasmin S. Phytochemical screening and antibacterial activity on Ricinus communis L. Plant SciFeed. 2011; 1:167-173.
Ullah H, Khan A, Baig MW, Ullah N, Ahmed N, Tipu MK, Ali H Khan S. Poncirin attenuates CCL4-induced liver injury through inhibition of oxidative stress and inflammatory cytokines in mice. Compl Med Ther. 2020; 20:115.
Hsu Y, Wang C, Lee M, Huang C. Hepatoprotection by Traditional Essence of Ginseng against Carbon Tetrachloride-Induced Liver Damage. Nutr. 2020; 12:3214.
Vishwanath J, Preetham GB, Anil KB, Satwik RJ. A Review on Hepatoprotective Plants. Int J Drug Dev Res. 2012; 4(3):1-8.
Kim SH, Cheon HJ, Yun N, Oh ST, Shin E, Shim KS. Protective Effect of a Mixture of Aloe vera and Silybum marianum against carbon tetrachloride–induced acute hepatotoxicity and liver fibrosis. J Pharmacol Sci. 2009; 109:119-127.
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