Comparative Study of Phytonutrient Content and Antioxidant Activity of the Fruit Juices of Watermelon (Citrullus lanatus) and Horned melon (Cucumis metuliferus) http://www.doi.org/10.26538/tjnpr/v7i8.32
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Abstract
This study was aimed at determining the vitamin, phytochemical compositions and the antioxidant properties of watermelon and horned melon fruit juices. The analyses were carried out using standard methods. The results obtained showed that the fruits contained vitamins C (0.13 mg/ml and 0.68 mg/ml), A (1.60 and 0.20 mg/ml), B1 (0.0011 and 0.00009 mg/ml), B2 (0.0005 and 0.00011 mg/ml), B3 (6.62 and 0.00007 mg/ml), B9 (0.12 and 0.0021 mg/ml) and E (3.48 and 0.00042 mg/ml) for fruit juice of watermelon and horned melon respectively. From the phytochemical analysis, phytate (0.33%), alkaloids (0.48 mg/ml), Oxalate (3.24 mg/ml) and
tannins (108.79 mg TAE/g) were detected only in the fruit juice of horned melon while flavonoid (3.45 mg/ml) and terpenoids (0.12 mg/ml) were detected only in the fruit juice of watermelon. Others were 0.17 and 0.06 mg/ml phenol, 0.21 and 0.46 mg/ml cardiac glycosides, 0.09 and 0.19 mg/ml saponin for fruit juices of watermelon and horned melon respectively. Horned melon juice showed no antioxidant activity up to a concentration of 100% while the fruit juice of watermelon showed varying activity at different concentrations. The EC50 for scavenging ability on DPPH and inhibition of lipid peroxidation were 40.0 and 76.0% respectively while OD0.5 for reducing power was 45.0 %. These results showed that watermelon juice is richer in phenols and flavonoids than horned melon juice. Therefore, watermelon juice could be used in pharmaceutical preparations.
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References
Chavan BB, Gadekar AS, Mehta PP, Vawhal PK, Kolsure Anti-aging Properties: A Comprehensive Review to prolong Human life. Antioxi. 2020; 9:1123.
Oluwagbenle HN, Adesina AJ, Aremu OI. Comparative Assessment of the proximate, mineral composition and mineral safety index of peel, pulp and seeds of Cucumber (Cucumis sativus). Open J Appl Sci. 2019; 9: 691-701.
Kyriacou MC, Leskovar DI, Colla G, Rouphael Y. Watermelon and Melon Fruit Quality: The Genotypic and Agro Environmental Factors Implicated. Sci Hortic, 2018; 30: 8-12.
Chuku EC, Emelike NJT. Comparative studies on the nutrient composition of water melon (Citrullus lunatus) and cucumber (Cucumis sativus) fruits and Associated fungi. Nig J Mycol, 2014; 6: 109 – 116.
Nwachoko N, Owhonda N. Proximate, Phytochemical and Antidiarrhoea Properties of water melon seeds. Arch Nutr, Pub Healt. 2019;1(1): 1-6.
Manivannan A, Lee ES, Han K, Lee HE, Kim DS. Versatile Nutraceutical Potentials of Watermelon-A Modest Fruit Loaded with Pharmaceutically Valuable Phytochemicals. Molecules. 2020; 25(22):5258.
Sovljanski O, Seregelj V, Pezo L, et al. Horned melon Pulp, Peel, and seed: New Insight into Phytochemical and Biological Properties. Antioxid. 2022; 11(5):825.
Viera EF, Grosso C, Rodrigues F, Moreira MM, Fernades VC, Delerue-matos C. Bioactive Compounds of Horned melon (Cucumis metuliferus E.Meyer ex Naudin). In: Murthy, HN, Peak KY, (ed) Bioactive Compounds in underutilized vegetables and legumes. Phyto. 2021; 341-361.
Salihovic M, Pazalja M, Ajanovic A. Antioxidant activity of Watermelon seeds determined by DPPH Assay. Kem.Ind. 2022; 77:5-6.
Sofowora A. Screening Plants for Bioactive Agents. In: Medicinal Plants and Traditional Medicines in Africa. 2nd ed. Spectrum Books Ltd., Sunshine House, Ibadan. 1993; p. 81-93.
Trease GE, Evans MC. Textbook on Pharmacognosy (13th Edn). Bailiere Tandal and Caussel, London. 1989; p. 144-148.
Harbone JB. Phytochemical Methods. A Guide to Modern Technique of Plant Analysis. Chapman and Hall Ltd, London. 1973; p. 49-188.
Barros L, Soraia F, Baptista P, Cristina F, Miguel VB, Isabel CF, Ferreira R. Antioxidant activity of Agaricus sp. mushrooms by chemical, biochemical and electrochemical assays. Food Chem. 2007; 111:61–66.
Obadoni BO, Ochuko PO. Phytochemical Studies and Comparative Efficacy of the crude Extracts of some Haemostatic Plants in Edo and Delta States of Nigeria. Glob J Pure Appl Sci, 2002; 8(2):203-208.
AOAC. Association of Analytical Chemistry. Methods for Phytochemical Analyses. 1990; 2318-2391.
Young SM, Greaves JS. Laboratory Handbook of Methods of Food Analysis, Leonard Hill, London. 1940; p. 217-273.
Harborne JB. Comparative Biochemistry of the Flavonoids. Academic Press, New York. 1995; p. 383.
Osagie AU. Anti-nutritional Factors. In: Nutritional Quality of Plant Foods. Ambik Press Ltd, Benin City, Nigeria. 1998; 1- 40:221-244.
Kirk R, Sawyer R. Perason’s composition and analysis of foods, 9th edition. Longman Scientific and Technical. 1991;p. 647-648.
Ebrahimzadeh MA, Seyed MN, Seyed FN, Fatemeh B, Ahmad RB. Antioxidant and free radical scavenging activity of H. officinalis, L.angustifolius, V. odorata, B. hyrcana and C. speciosum. Pak J Pharm Sci. 2009; 23(1): 29-34.
Treml J, Smejkal K. Flavonoids as Potent Scavengers of Hydroxyl Radicals. 2016; 15(4):720-738.
Babar Q, Ali A, Saeed A, Tahir MF. Novel Treatment Strategy against COVID-19 through Anti-Inflammatory, Antioxidant and Immunostimulatory Properties of the B vitamin Complex In (ed.): B-Complex Vitamins - Sources, Intakes and Novel Applications. IntechOpen. 2021; DOI: http://dx.doi.org/10.5772/intechopen.100251.
Huang Z, Liu Y, Qi G, Brand D, Zheng SG. Role of Vitamin A in the immune system. J Clin Med. 2018; 7(9):258.
Gholizadeh M, Basafa Roodi P, Abaj F, et al. Influence of Vitamin A supplementation on inflammatory biomarkers in adults: a systematic review and meta-analysis of randomized clinical trials. Sc Rep. 2022; 12, 21384.
Zheng XY, Liang J, Li YS, Tu M. Role of Fat-Soluble vitamins in Osteoarthritis Management. J Clin Rheumatol: practical reports on rheumatic and musculoskeletal diseases, 2018; 24(3), 132-137.
Tungmunnithum D, Thongboonyou A, Pholboon A, Yangsabai A. Falvonoids and other Phenolic Compounds for Medicinal Plants for Pharmaceutical and Medical Aspects: An Overview. Medicines. 2018; 5(3):93.
Munteanu IG, Apetrei C. Analytical methods used in Determining Antioxidant Activity: A Review. Int J Mol Sci. 2021; 22(7):3380.
Arro-Diaz DJ, Castelnaux-Ochoa N, Ochoa-Pacheco A,DoNascimento YM. Antioxidant activity of Bioactive compounds isolated from leaves and bark of Gymnanthes lucida Sw. Bev Cuba Quim. 2020; 33(1): 2239.
Su LJ, Shang JH, Gomez H, Murugan R, Hong X, Xu D, Jiang F, Peng ZY. Reactive Oxygen Species-Induced Lipid Peroxidation in Apoptosis, Autophagy, and Ferroptosis. Oxid Med Cell longev. 2019.
Yin H, Xu L, Porter NA. Free Radical Lipid Peroxidation: Mechanism and Analysis. Chem Rev. 2011; 111: 5944-5972.
Petrovic S, Arsic A, Ristic-Medic D, Cvetkovic Z, Vucic V. Lipid peroxidation and antioxidant Supplementation in Neurogenerative Diseases: A Review of Human Studies. Antioxidants. 2020; 9(11):1128.