<i>Gongronema latifolium</i> Ethanol Crude Leaf Extract Ameliorates Organ Injuries Via Anti-Inflammatory and Anti-Diabetic Mechanisms in Streptozotocin-Induced Diabetic Rats

http://www.doi.org/10.26538/tjnpr/v7i8.38

Authors

  • Godfrey O. Asuquo Department of Histopathology and cytology, Faculty of Medical Laboratory Science, University of Calabar, P. M. B. 1115, Calabar Nigeria
  • Mfoniso I. Udonkang Department of Histopathology and cytology, Faculty of Medical Laboratory Science, University of Calabar, P. M. B. 1115, Calabar Nigeria
  • Yibala I. Oboma Department of Medical Laboratory Science, Faculty of Basic Medical Sciences, Niger Delta University, Wilberforce Island, P.M.B 071 Bayelsa State, Nigeria
  • Iya E. Bassey Department of Clinical Chemistry and Immunology, Faculty of Medical Laboratory Science, University of Calabar, P. M. B. 1115, Calabar Nigeria
  • Beshe S. Beshe Department of Zoology and Environmental Biology, Faculty of Biological Sciences, University of Calabar, P. M. B. 1115, Calabar Nigeria
  • Obioma O. Okechi Department of Medical Laboratory Science, Abia State University, P. M. B. 2000, Uturu Abia State, Nigeria.
  • Daniel U. Owo Department of Physiology, University of Calabar, P. M. B. 1115, Calabar Nigeria.
  • Grace S. Effiong Department of Biochemistry, Faculty Of Science , University of Uyo, P. M. B. 1017, Uyo, Nigeria.

Keywords:

Malondialdehyde, interleukin-6, C-reactive protein, Diabetes mellitus, Gongronema latifolium

Abstract

In Streptozotocin-induced diabetes mellitus rats, the effects of Gongronema latifolium (GL)leaf extract on the histology of some organs, hyperglycemia, and inflammatory markers were investigated. Five (5) groups of 25 male rats (weighing 150g and 200g each) were created and group one received distilled water. Group 2 received GL extract at a dose of 200 mg/kg. Group 3 diabetic rats were only given access to water and food. Diabetes-induced rats in Group 4 received 200 mg/kg of GL extract. In group 5 rats, subcutaneous insulin (0.14 ml/kg) was given to the diabetic rats. The DM-induced group (t=3.892) had statistically higher plasma glucose levels (p=0.018) than the control group. In comparison to the diabetic non-treated group, the treatment groups receiving insulin (t=2.825, P=0.048) and extract (t=2.731, P=0.052) demonstrated a statistically significant decrease in plasma glucose level. The test groups showed lower serum levels of C-reactive protein (F=0.680, P=0.863), interleukin-6 (F=0.484, P=0.392), and MDA (F=0.347, P=0.108). Interleukin-6 and C-reactive protein levels in Gongronema latifolium leaf extract were reduced, demonstrating the plant's anti-inflammatory capabilities. Compared to the control group, the treated groups visceral organ histomorphology displayed characteristics
compatible with normal histology of the organs. The group with diabetes mellitus-induced had glomeruli with focal regions of haemorrhage In the pancreas tissue of the diabetic non-treated group, cellular degradation was seen, indicating damage. Untreated diabetics exhibited a clogged central vein and an invasion of inflammatory cells. According to this study, Gongronema latifolium has anti-hyperglycemia and anti-inflammatory capabilities and reduces cellular changes in the visceral organs. 

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Published

2023-08-31

How to Cite

Asuquo, G. O., Udonkang, M. I., Oboma, Y. I., Bassey, I. E., Beshe, B. S., Okechi, O. O., … Effiong, G. S. (2023). <i>Gongronema latifolium</i> Ethanol Crude Leaf Extract Ameliorates Organ Injuries Via Anti-Inflammatory and Anti-Diabetic Mechanisms in Streptozotocin-Induced Diabetic Rats: http://www.doi.org/10.26538/tjnpr/v7i8.38. Tropical Journal of Natural Product Research (TJNPR), 7(8), 3823–3828. Retrieved from https://tjnpr.org/index.php/home/article/view/2454