Gongronema latifolium Ethanol Crude Leaf Extract Ameliorates Organ Injuries Via Anti-Inflammatory and Anti-Diabetic Mechanisms in Streptozotocin-Induced Diabetic Rats http://www.doi.org/10.26538/tjnpr/v7i8.38
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Abstract
In Streptozotocin-induced diabetes mellitus rats, the effects of Gongronema latifolium (GL)leaf extract on the histology of some organs, hyperglycemia, and inflammatory markers were investigated. Five (5) groups of 25 male rats (weighing 150g and 200g each) were created and group one received distilled water. Group 2 received GL extract at a dose of 200 mg/kg. Group 3 diabetic rats were only given access to water and food. Diabetes-induced rats in Group 4 received 200 mg/kg of GL extract. In group 5 rats, subcutaneous insulin (0.14 ml/kg) was given to the diabetic rats. The DM-induced group (t=3.892) had statistically higher plasma glucose levels (p=0.018) than the control group. In comparison to the diabetic non-treated group, the treatment groups receiving insulin (t=2.825, P=0.048) and extract (t=2.731, P=0.052) demonstrated a statistically significant decrease in plasma glucose level. The test groups showed lower serum levels of C-reactive protein (F=0.680, P=0.863), interleukin-6 (F=0.484, P=0.392), and MDA (F=0.347, P=0.108). Interleukin-6 and C-reactive protein levels in Gongronema latifolium leaf extract were reduced, demonstrating the plant's anti-inflammatory capabilities. Compared to the control group, the treated groups visceral organ histomorphology displayed characteristics
compatible with normal histology of the organs. The group with diabetes mellitus-induced had glomeruli with focal regions of haemorrhage In the pancreas tissue of the diabetic non-treated group, cellular degradation was seen, indicating damage. Untreated diabetics exhibited a clogged central vein and an invasion of inflammatory cells. According to this study, Gongronema latifolium has anti-hyperglycemia and anti-inflammatory capabilities and reduces cellular changes in the visceral organs.
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