Evaluation of α-Amylase Inhibitory Activity of Saponins from Panax bipinnatifidus Seem. Growing in Vietnam http://www.doi.org/10.26538/tjnpr/v7i8.27
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Abstract
α-Amylase inhibitors represent a drug target for lowering postprandial hyperglycemia in diabetic patients. According to traditional medicines, Panax plants have been utilised to treat diabetes and considered as a valuable source to develop anti-diabetic supplementary products. The current study aimed to evaluate the α-amylase inhibitory activities of Panax bipinnatifidus root cultivated
in Vietnam and its main chemical constituents. The two major saponins, Araloside A and Stipuleanoside R2 were confirmed by ultra performance liquid chromatography quadruple-time of flight-mass spectrometry (UPLCQ-TOF/MS) and nuclear magnetic resonance (NMR). Then, the results indicated that Araloside A and Stipuleanoside R2, were shown to moderately inhibit the α-amylase activity in an enzymatic assay with IC50 values of 781.46 and 2746.90 μg/mL, respectively. Moreover, Araloside A (Vmax ~ 0.0635 µM/min, Km ~ 4.691 µM) was displayed as a completive inhibitor of α-amylase in the kinetic study whereas Acarbose (Vmax ~ 0.053 µM/min, Km ~ 6.521 µM) showed a mixed inhibition. This study suggested the posibility of Araloside A acting as an α-amylase inhibitor. Also, it supports the traditional use of P. bipinnatifidus in antidiabetic dietary.
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