Evaluation of α-Amylase Inhibitory Activity of Saponins from Panax bipinnatifidus Seem. Growing in Vietnam

http://www.doi.org/10.26538/tjnpr/v7i8.27

Authors

  • Hieu N. Ngoc Faculty of Pharmacy, Phenikaa University, Hanoi 12116, Vietnam
  • Nguyen T.V. Anh Faculty of Biotechnology, Chemistry and Environmental Engineering, Phenikaa University, Hanoi 12116 Vietnam
  • Nguyen L. Nhi Faculty of Biotechnology, Chemistry and Environmental Engineering, Phenikaa University, Hanoi 12116 Vietnam
  • Dang T. Yen Faculty of Pharmacy, Phenikaa University, Hanoi 12116, Vietnam
  • Vu V. Tuan Faculty of Pharmacy, Phenikaa University, Hanoi 12116, Vietnam
  • Nguyen H. Tung Faculty of Pharmacy, Phenikaa University, Hanoi 12116, Vietnam
  • Huong T.X Hoang Faculty of Nursing, Phenikaa University, Hanoi 12116, Vietnam
  • Luan Chu Faculty of Biotechnology, Chemistry and Environmental Engineering, Phenikaa University, Hanoi 12116 Vietnam

Keywords:

antidiabetic, α-amylase, Stipuleanoside R2, Araloside A., saponins, Panax bipinnatifidus

Abstract

α-Amylase inhibitors represent a drug target for lowering postprandial hyperglycemia in diabetic patients. According to traditional medicines, Panax plants have been utilised to treat diabetes and considered as a valuable source to develop anti-diabetic supplementary products. The current study aimed to evaluate the α-amylase inhibitory activities of Panax bipinnatifidus root cultivated
in Vietnam and its main chemical constituents. The two major saponins, Araloside A and Stipuleanoside R2 were confirmed by ultra performance liquid chromatography quadruple-time of flight-mass spectrometry (UPLCQ-TOF/MS) and nuclear magnetic resonance (NMR). Then, the results indicated that Araloside A and Stipuleanoside R2, were shown to moderately inhibit the α-amylase activity in an enzymatic assay with IC50 values of 781.46 and 2746.90 μg/mL, respectively. Moreover, Araloside A (Vmax ~ 0.0635 µM/min, Km ~ 4.691 µM) was displayed as a completive inhibitor of α-amylase in the kinetic study whereas Acarbose (Vmax ~ 0.053 µM/min, Km ~ 6.521 µM) showed a mixed inhibition. This study suggested the posibility of Araloside A acting as an α-amylase inhibitor. Also, it supports the traditional use of P. bipinnatifidus in antidiabetic dietary.

Author Biography

Luan Chu, Faculty of Biotechnology, Chemistry and Environmental Engineering, Phenikaa University, Hanoi 12116 Vietnam

Faculty of Biomedical Sciences, Phenikaa University, Hanoi 12116, Vietnam

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Published

2023-08-31

How to Cite

Ngoc, H. N., Anh, N. T., Nhi, N. L., Yen, D. T., Tuan, V. V., Tung, N. H., … Chu, L. (2023). Evaluation of α-Amylase Inhibitory Activity of Saponins from Panax bipinnatifidus Seem. Growing in Vietnam: http://www.doi.org/10.26538/tjnpr/v7i8.27. Tropical Journal of Natural Product Research (TJNPR), 7(8), 3756–3760. Retrieved from https://tjnpr.org/index.php/home/article/view/2447