Activities of <i>Hydrocotyle sibthorpioides</i> Lam. Extract in Capsule on Natural Killer and CD8 Cells in Human


  • Salman Umar Departmen of Pharmaceutical, Faculty of Pharmacy, Universitas Andalas, Limau Manis, Pauh, Padang, West Sumatra, Indonesia
  • Sri Gusriyani Student of Magister Program of Pharmacy, Faculty of Pharmacy, Universitas Andalas Limau Manis, Pauh, Padang, West Sumatra, Indonesia
  • Afriwardi Afriwardi Department of Physiology, Faculty of Medicine, Universitas Andalas, Limau Manis, Pauh, Padang, West Sumatra, Indonesia
  • Yufri Aldi Department of Pharmacology, Faculty of Pharmacy, Universitas Andalas, Limau Manis, Pauh, Padang, West Sumatra, Indonesia


NK cell, Immunostimulant, Hydrocotyle sibthorpioides Lam., Clinical trial, CD8 cell


Hydrocotyle sibthorpioides Lam. has been used by Indonesians to increase immunity. The present study was therefore conducted to investigate the effect of Hydrocotile sibthorpioides Lam. extract in capsules on the activity of natural killer (NK) and CD8 cells in humans during the first phase of clinical trials. Twenty participants were recruited for the study and divided into two groups. The first group received nothing except an additional ingredient as a placebo. Also, the second group was administered a preparation test at a dose of 67 mg per person. Blood was obtained before and after the test preparation was administered, which was done once daily for three days. The protein concentrations of NK and CD8 cells were measured by the enzyme-linked immunosorbent assay (ELISA) kit. The results showed that the average activity of NK cells before giving the preparations to the first group was 81.644 U/mL, and after administration was 81.910 U/mL. In contrast, the average activity of NK cells of the second group was 81.998 and 83.541U/mL, respectively. The concentrations of CD8 cells in the first group were 230.160 and 235.575U/mL before and after administration, respectively. Compared to the second group, the concentrations of CD8 cells were 238.407 and 335.323 U/mL, respectively. The findings of this study indicate that the H. sibthorpioides extract could increase the NK and CD8 cellular activities, which may improve the body's capacity to destroy virus invaders. 


Marbun R, Suwarso E, Yuandani Y. Immunomodulatory Effects of Ethanol Extract of Artemisia Vulgaris L. in Male Rats. Asian J Pharm Clin Res. 2018; 11 (Special issue 1):245-247

Hasdianah DP, Peristiowati IS, Imam S. Imunologi diagnosis dan teknik biologi molekuler. Yogyakarta: Nuha Medika; 2014; 3-10

Radji M. Imunologi & Virologi. Jakarta: PT ISFI Pcnerbitan. 2010.

Sudiono J. Sistem Kekebalan Tubuh. Jakarta: EGC. 2014;52-54.

Kresno SB. Diagnosis dan Prosedur Laboratorium Imunologi. Jakarta: FKUI. 2010.

Husni E, Badriyya E, Putri L, Aldi Y. The Effect of Ethanol Extract of Moringa Leaf (Moringa oleifera Lam) Against the Activity and Capacity of Phagocytosis of Macrofag Cells and the Percentage of Leukosit Cells of White Mice. Pharmacogn J. 2021; 13(3): 706-712.

Huang Q, Zhang S, Huang R, Wei L, Chen Y, Lv S, Liang C, Tan S, Liang S, Zhuo L, Lin X. Isolation and identification of an anti-hepatitis B virus compound from Hydrocotyle sibthorpioides Lam. J Ethnopharmacol. 2013;150(2):568-575.

Yu F, Yu F, McGuire PM, Li R, Wang R. Effects of Hydrocotyle sibthorpioides extract on transplanted tumors and immune function in mice. Phytomed. 2007; 14(2-3):166-171.

Afriwardi A, Nazmi R, Dillasamola D, Badriyya E, Aldi Y. Anti-inflammatory activity of Pegagan Embun (Hydrocotyle sibthorpioides Lam.) plant extract on topical application. Pharmacogn J. 2021;13(2): 411-417.

Umar S, Erman NP, Badriyya E, Aldi Y. The Activities of Pegagan Embun (Hydrocotyle sibthorpioides Lam.) on TNF- α, Macrophages and Leukocytes Male White Mice Exposed by H5N1 Virus Antigens. Pharmacogn J. 2022; 14(2): 253-261.

Husni E, Dillasamola D, Badriyya E, Angelia R, Aldi Y. Ethanol Extract Activity of Pegagan Embun (Hydrocotyle sibthorpioides L.) Against Hematopoietic on Anemic Male White Mice. Pharmacogn J. 2021; 13(4): 866-873.

Afriwardi, Abdillah, R., Husni, E., Hardini, H., Zuler, K. T. S., Alianta, A. A., & Aldi, Y. (2022). Subacute Toxicity Test of Hydrocotyle Sibthorpioides Lam. Extract on Histopathological Images of Liver and Kidney of White Male Mice. Pharmacognosy Journal. 14(5); 619–626.

Baddriya E, Latifah W, Aldi Y. Sub-Acute Toxicity Study of Pegagan Embun (Hydrocotyle Sibthorpioides Lam.) Extract on The SGPT and SGOTt Level of Wistar White Male Rats. Int J App Pharm. 2023; 15(Special issue 1): 5-9.

Afriwardi, Fitri, A., Husni, E., Badriyya, E., & Aldi, Y. (2021). Immunostimulant Activity of Pegagan Embun Herbs Extract (Hydrocotyle sibthorpioides Lam.) With Carbon Clearance Method Towards Male White Mice. Pharmacogn J. 13: 1472–1477.

Aldi Y, Afriwardi A, Badriyya E, Azukhruf W. S, Alianta A. A. Effects of Pegagan Embun (Hydrocotyle sibthorpioides Lam) Extract on Renal Function in Male Wistar Rats as Assessed by Creatinine Clearance. Trop J of Nat Prod Res. 2023; 7(3): 2547-2550.

Santoso B. Suryawati S, Saleh D S. Evaluasi Khasiat dan Keamanan Obat (Uji klinik), dalam Farmakologi Klinik dan Farmakoterapi. Jogjakarta: UGM Press. 2006; 183-189.

Depkes RI. Parameter Standar Umum Ekstrak Tumbuhan Obat. Jakarta: Departemen Kesehatan Republik Indonesia; 2000.

Harbone, J. B. Metode Fitokimia: Penuntun Cara Modern Menganalisa Tumbuhan (translated by: Kosasih Padmawinata & Iwang Soediro). Bandung: Penerbit ITB; 2006.

Middleton E, Kandaswami C, Theoharides TC. The effects of plant flavonoids on mammalian cells: implications for inflammation, heart disease, and cancer. Pharmacol Rev. 2000; 52(4): 673-751.

Hanifah L, Kiptiyah K. Potensi kesambi (Scheichera oleosa) sebagai kandidat imunomodulator. In Prosiding Seminar Nasional Biologi. 2020 Sep 3: 6(1); 119-126.

Abbas AK, Lichtman AH, Pillai S. Basic Immunology: Functions and Disorders of the Immune System, 6e: Sae-EBook. Elsevier India; 2019.

Bruunsgaard H, Pedersen AN, Schroll M, Skinhøj P, Pedersen BK. Proliferative responses of blood mononuclear cells (BMNC) in a cohort of elderly humans: role of lymphocyte phenotype and cytokine production. Clin Exp Immunol. 2000;119(3):433-40.

Kumar V, Cotran RS, Robbins SL. Buku ajar patologi 7nd ed, Vol. 2. Jakarta: Penerbit Buku Kedokteran EGC; 2007.



How to Cite

Umar, S., Gusriyani, S., Afriwardi, A., & Aldi, Y. (2023). Activities of <i>Hydrocotyle sibthorpioides</i> Lam. Extract in Capsule on Natural Killer and CD8 Cells in Human: Tropical Journal of Natural Product Research (TJNPR), 7(8), 3766–3769. Retrieved from

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