Synthesis, Characterization, and Application of Molecularly Imprinted Polymer - Modified Silica Gel for Andrographolide Purification from <i>Andrographis paniculata</i> (Burm.f.) Nees Methanol Extract

http://www.doi.org/10.26538/tjnpr/v7i8.28

Authors

  • Wiwin Winingsih Departement of Pharmacochemistry, Sekolah Tinggi Farmasi Indonesia Bandung, 40266, Indonesia
  • Adang firmansyah Departement of Pharmacochemistry, Sekolah Tinggi Farmasi Indonesia Bandung, 40266, Indonesia
  • Rayyan Ayesha Departement of Pharmacochemistry, Sekolah Tinggi Farmasi Indonesia Bandung, 40266, Indonesia
  • Purri A. Novwanti Departement of Pharmacochemistry, Sekolah Tinggi Farmasi Indonesia Bandung, 40266, Indonesia
  • Lucia N.L.T. Bahy Departement of Pharmacochemistry, Sekolah Tinggi Farmasi Indonesia Bandung, 40266, Indonesia

Keywords:

Silica gels, Surface Imprinting, Andrographolide, Modified Silica Gel

Abstract

Molecularly Imprinted Polymer- Modified silica is silica gel coated with Molecularly Imprinted Polymer (MIP) which has cavities with the same shape, size, and functional groups as the template molecule, allowing for selective absorption. Therefore, this study aimed to synthesize, characterize, and apply MIP-modified silica for purification of andrographolide from the Andrographis paniculata (Burm.f.) Nees methanol extract. To synthesize the MIP-modified silica, the surface imprinting method was employed, with APTES, TEOS, acetic acid, and andrographolide, as the functional monomer, cross-linking agent, catalyst, and template molecule, respectively. Modified silica was characterized using FTIR and SEM. Its performance was tested by determining the imprinting factor. The purity of purified methanol extract was tested using HPLC. FTIR and SEM characterization confirmed the presence of MIP polymer on the surface of
silica gel. Its imprinting factor was 1.22. Purification results of the Andrographis paniculata (Burm.f.) Nees extract demonstrated that modified silica improved the purity of methanol extract from 1.46 % to 65% 

References

Jayakumar T, Hsieh C-Y, Lee J-J, Sheu J-R. Experimental and clinical pharmacology of Andrographis paniculata and its major bioactive phytoconstituent andrographolide. J Clin Pharm Ther. 2013; 2013 :1-16.

Okhuarobo A, Falodun JE, Erharuyi O, V Imieje, Falodun A, Langer P. Harnessing the medicinal properties of Andrographis paniculata for diseases and beyond: a review of its phytochemistry and pharmacology, Asian Pac. J. Trop. Dis., 2014; 4 (3):213-222

Husori, D., Patilaya, P., A. Panjaitan, D., & A. Shulha, I. Antisecretory Effect of the Combination of Pegagan (Centella Asiatica) and Sambiloto (Andrographis paniculata) Leaves Ethanol Extracts on Pyloric Ligation-Induced Gastric Ulcer in Rats. Trop. J. Nat. Prod., 2021;5(10):1743–1746.

Ajayi OS, Balogun OS, Olawuni IJ, October N, Adigun R, Akinlade IG. Alpha Amylase Inhibition and Antioxidant Activities of Bicyclic Diterpenoid Lactones from Andrographis paniculata. Trop. J. Nat. Prod., 2021; 5(6): 1110–1117.

Pundarikakshudu K. A Simple and Facile Method for the Isolation of Andrographolide from Andrographis Paniculata Nees. Am. j. PharmTech res. 2016;6(6):266–275.

Winingsih W, Ibrahim S, Damayanti S. Development of molecularly imprinted polymer microspheres made by surface imprinting for purification of andrographolide. Rasayan J Chem. 2022;15(2):1296-1305.

Renkecz T, László K, Horváth V. Molecularly imprinted microspheres prepared by precipitation polymerization at high monomer concentrations. Mol.Imprint. 2014;2(1):1–17.

Scott RPW. Silica gel and bonded phases: their production, properties and use in LC (Vol. 4). Wiley, 1993.

Yang P, Hou WD, Qiu HD, Liu X, Jiang SX. Preparation of quercetin imprinted core–shell organosilicate microspheres using surface imprinting technique. Chin. Chem. Lett.. 2012;23(5):615–618.

Yang ZY, Ronghuo ZS, Rong LYL. Production Technique of Andrographolide and Neoandrographolide, dehydroandrogapholide.Leiyunshang Pharmaceutical Group.Patent CN101559088A. 2012.

Winingsih W, Ibrahim S, Damayanti S. Purification of Andrographolide Methanolic Extract Using Molecularly Imprinted Polymer Prepared by Precipitation Polymerization. Sci Pharm. 2022;90(2).

Zhang L, Zhang H, Li L, Zuo P, Zhao F, Liu M, Ye BC, Li Y. Pneumocandin B 0 -imprinted polymer using surfaceimprinting technique for efficient purification of crude product. Anal Sci. 2016;32(9):923–930.

He HX, Gan Q, Feng CG. An Ion-imprinted Silica Gel Polymer Prepared by Surface Imprinting Technique Combined with Aqueous Solution Polymerization for Selective Adsorption of Ni (II) from Aqueous Solution. Chin. J. Polym. Sci., 2018;36(4):462–471.

Liu H, Zhou Y, Qi Y, Sun Z, Gong B. Preparation of thiamphenicol magnetic surface molecularly imprinted polymers for its selective recognition of thiamphenicol in milk samples. J. Liq. Chromatogr. Relat. Technol.. 2018;41(13–14):868–879.

Liu J, Deng Q, Tao D, Yang K, Zhang L, Liang Z, Zhang Y. Preparation of protein imprinted materials by hierarchical imprinting techniques and application in selective depletion of albumin from human serum. Sci Rep. 2014; 4:1–6.

Zhang C, Wang Y, Zhou Y, Guo J, Liu Y. Silica-based surface molecular imprinting for recognition and separation of lysozymes. Anal Methods. 2014;6(21):8584–8591.

Zhi K, Wang L, Zhang Y, Jiang Y, Zhang L, Yasin A. Influence of size and shape of silica supports on the sol-gel surface molecularly imprinted polymers for selective adsorption of gossypol. Materials. 2018;11(5):1–16.

Hasanah AN, Safitri N, Zulfa A, Neli N, Rahayu, D. Factors affecting preparation of molecularly imprinted polymer and methods on finding template-monomer interaction as the key of selective properties of the materials. Molecules. 2021;26(18).

Published

2023-08-31

How to Cite

Winingsih, W., firmansyah, A., Ayesha, R., Novwanti, P. A., & Bahy, L. N. (2023). Synthesis, Characterization, and Application of Molecularly Imprinted Polymer - Modified Silica Gel for Andrographolide Purification from <i>Andrographis paniculata</i> (Burm.f.) Nees Methanol Extract: http://www.doi.org/10.26538/tjnpr/v7i8.28. Tropical Journal of Natural Product Research (TJNPR), 7(8), 3761–3765. Retrieved from https://tjnpr.org/index.php/home/article/view/2444