Synthesis, Characterization, and Application of Molecularly Imprinted Polymer - Modified Silica Gel for Andrographolide Purification from Andrographis paniculata (Burm.f.) Nees Methanol Extract http://www.doi.org/10.26538/tjnpr/v7i8.28
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Abstract
Molecularly Imprinted Polymer- Modified silica is silica gel coated with Molecularly Imprinted Polymer (MIP) which has cavities with the same shape, size, and functional groups as the template molecule, allowing for selective absorption. Therefore, this study aimed to synthesize, characterize, and apply MIP-modified silica for purification of andrographolide from the Andrographis paniculata (Burm.f.) Nees methanol extract. To synthesize the MIP-modified silica, the surface imprinting method was employed, with APTES, TEOS, acetic acid, and andrographolide, as the functional monomer, cross-linking agent, catalyst, and template molecule, respectively. Modified silica was characterized using FTIR and SEM. Its performance was tested by determining the imprinting factor. The purity of purified methanol extract was tested using HPLC. FTIR and SEM characterization confirmed the presence of MIP polymer on the surface of
silica gel. Its imprinting factor was 1.22. Purification results of the Andrographis paniculata (Burm.f.) Nees extract demonstrated that modified silica improved the purity of methanol extract from 1.46 % to 65%
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