Phenolic Profiling with Antioxidant Activity of Thai Traditional Medicines: Phikud Bencha Thien, Kot and Keson Remedies http://www.doi.org/10.26538/tjnpr/v7i8.23
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Abstract
Phikud Bencha Thien (BT), Phikud Bencha Kot (BK), and Phikud Bencha Keson (BKS) are three of the most well-known compositions in Thai traditional medicine and are included in a wide range of Thai polyherbal formulas. Each individual formulation has potential as a rich source of natural antioxidants, especially polyphenolic compounds. The present study determined the total phenolic content, total flavonoid content, and antioxidant capacity of water and methanol extracts of these three remedies as well as identifying the phenolic acid and flavonoid compounds present in these remedies using HPLC. Antioxidant activity was determined using DPPH and ABTS reduction assays, the ferric reducing antioxidant power (FRAP) assay, and the oxygen radical absorbance capacity (ORAC) assay. Principal Component Analysis and Hierarchical Cluster Analysis were used to classify relationships between the extract contents and antioxidant activity. Methanol extracts had the highest total phenolic and total flavonoid contents. Antioxidant activities in each assay varied between methanol and water extracts. The BKS extract exhibited significantly stronger antioxidant activity than the BT and BK extracts in all antioxidant models. Caffeic acid was found in high amounts in BT and BK extracts, whereas gallic acid was found as
the major constituent of BKS extract. Multivariate analysis revealed that methanol and water extracts of BKS displayed the highest antioxidant capacities and had the highest phytochemical contents.
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