Phenolic Profiling with Antioxidant Activity of Thai Traditional Medicines: Phikud Bencha Thien, Kot and Keson Remedies

http://www.doi.org/10.26538/tjnpr/v7i8.23

Authors

  • Suthida Datham Graduate School in the Program of Thai Traditional Pharmacy Program, Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand
  • Ploenthip Puthongking Division of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand
  • Juthamat Ratha Melatonin Research Group, Khon Kaen University, 40002 Khon Kaen, Thailand
  • Pimolwan Siriparu Melatonin Research Group, Khon Kaen University, 40002 Khon Kaen, Thailand
  • Tanit Padumanonda Melatonin Research Group, Khon Kaen University, 40002 Khon Kaen, Thailand

Keywords:

principal component analysis, phenolics, antioxidant, Thai traditional medicine, phikud

Abstract

Phikud Bencha Thien (BT), Phikud Bencha Kot (BK), and Phikud Bencha Keson (BKS) are three of the most well-known compositions in Thai traditional medicine and are included in a wide range of Thai polyherbal formulas. Each individual formulation has potential as a rich source of natural antioxidants, especially polyphenolic compounds. The present study determined the total phenolic content, total flavonoid content, and antioxidant capacity of water and methanol extracts of these three remedies as well as identifying the phenolic acid and flavonoid compounds present in these remedies using HPLC. Antioxidant activity was determined using DPPH and ABTS reduction assays, the ferric reducing antioxidant power (FRAP) assay, and the oxygen radical absorbance capacity (ORAC) assay. Principal Component Analysis and Hierarchical Cluster Analysis were used to classify relationships between the extract contents and antioxidant activity. Methanol extracts had the highest total phenolic and total flavonoid contents. Antioxidant activities in each assay varied between methanol and water extracts. The BKS extract exhibited significantly stronger antioxidant activity than the BT and BK extracts in all antioxidant models. Caffeic acid was found in high amounts in BT and BK extracts, whereas gallic acid was found as
the major constituent of BKS extract. Multivariate analysis revealed that methanol and water extracts of BKS displayed the highest antioxidant capacities and had the highest phytochemical contents.

Author Biographies

Ploenthip Puthongking, Division of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand

Melatonin Research Group, Khon Kaen University, 40002 Khon Kaen, Thailand

Tanit Padumanonda, Melatonin Research Group, Khon Kaen University, 40002 Khon Kaen, Thailand

Division of Pharmacognosy and Toxicology, Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand

 

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Published

2023-08-31

How to Cite

Datham, S., Puthongking, P., Ratha, J., Siriparu, P., & Padumanonda, T. (2023). Phenolic Profiling with Antioxidant Activity of Thai Traditional Medicines: Phikud Bencha Thien, Kot and Keson Remedies: http://www.doi.org/10.26538/tjnpr/v7i8.23. Tropical Journal of Natural Product Research (TJNPR), 7(8), 3726–3730. Retrieved from https://tjnpr.org/index.php/home/article/view/2442

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