Antioxidant and Hepatoprotective Activities of Paris polyphylla Sm. Rhizome Extract in Cyclophosphamide-Induced Hepatic Damaged Mice http://www.doi.org/10.26538/tjnpr/v7i8.13
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Abstract
Paris polyphylla Smith (Melanthiaceae) is a medicinal plant for treating inflammatory, cancer, and liver-related diseases. Information on in vivo hepatoprotective effects of P. polyphylla rhizome extract has not been reported in the literature. The purpose of this study is to determine the antioxidant and hepatoprotective effects of P. polyphylla rhizome extract in cyclophosphamide-induced hepatic damaged mice. The beneficial effects on the liver of P. polyphylla extract have been evaluated through biochemical indicators (AST, ALT). Antioxidant activity was assessed by measuring levels of hepatic malondialdehyde (MDA) and antioxidant glutathione (GSH). A histopathological examination was also performed. P. polyphylla extract had DPPH free radical scavenging and lipid peroxidation inhibitory activity with IC50 values of 389.15 ± 9.09 µg/mL, 164.06 ± 8.32 µg/mL, respectively. Simultaneously, ALT, AST, and MDA
contents decreased, whereas that of liver GSH of mice increased when they received oral doses of 0.75 and 1.5 mg/g. Histopathological analysis of the liver revealed a significant hepatoprotective effect of P. polyphylla extract (1.5 mg/g) with evidence that no histopathological damage was found, there was a reduction in lipid changes, and the degree of necrosis in the cells was reduced.
The findings in my control study are evidence of the hepatoprotective potential of P. polyphylla extract, indicating that it might be used to prevent and treat liver-related diseases.
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