Antioxidant and Hepatoprotective Activities of Paris polyphylla Sm. Rhizome Extract in Cyclophosphamide-Induced Hepatic Damaged Mice


  • An G.T. Nguyen Faculty of Biology, College of Education, Vinh University, Nghe An Province, Vietnam
  • Hieu T. Tran Department of Chemistry, College of Education, Vinh University, Nghe An Province, Vietnam
  • Chen V. Tran Faculty of Traditional Medicine, University of Medicine and Pharmacy at Ho Chi Minh City, Vietnam
  • Binh N.D. Pham Van Lang High-tech Research and Development Institute, Van Lang University, Ho Chi Minh City, Vietnam
  • Hieu N. Nguyen Faculty of Pharmacy, PHENIKAA University, Hanoi, Vietnam
  • Hoa N. Nguyen Stroke Center, Nghean General Friendship Hospital, Nghe An Province, Vietnam
  • Khoa V. Vu Surgeon of Orthopedic and Trauma, Deputy Department of Orthopedic and Outpatient Treatment, Vietduc University Hospital, Ha Noi, Vietnam


Paris polyphylla Sm, Lipid peroxidation, Hepatoprotection, Cyclophosphamide, Antioxidant


Paris polyphylla Smith (Melanthiaceae) is a medicinal plant for treating inflammatory, cancer, and liver-related diseases. Information on in vivo hepatoprotective effects of P. polyphylla rhizome extract has not been reported in the literature. The purpose of this study is to determine the antioxidant and hepatoprotective effects of P. polyphylla rhizome extract in cyclophosphamide-induced hepatic damaged mice. The beneficial effects on the liver of P. polyphylla extract have been evaluated through biochemical indicators (AST, ALT). Antioxidant activity was assessed by measuring levels of hepatic malondialdehyde (MDA) and antioxidant glutathione (GSH). A histopathological examination was also performed. P. polyphylla extract had DPPH free radical scavenging and lipid peroxidation inhibitory activity with IC50 values of 389.15 ± 9.09 µg/mL, 164.06 ± 8.32 µg/mL, respectively. Simultaneously, ALT, AST, and MDA
contents decreased, whereas that of liver GSH of mice increased when they received oral doses of 0.75 and 1.5 mg/g. Histopathological analysis of the liver revealed a significant hepatoprotective effect of P. polyphylla extract (1.5 mg/g) with evidence that no histopathological damage was found, there was a reduction in lipid changes, and the degree of necrosis in the cells was reduced.
The findings in my control study are evidence of the hepatoprotective potential of P. polyphylla extract, indicating that it might be used to prevent and treat liver-related diseases. 


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How to Cite

Nguyen, A. G., Tran, H. T., Tran, C. V., Pham, B. N., Nguyen, H. N., Nguyen, H. N., & Vu, K. V. (2023). Antioxidant and Hepatoprotective Activities of Paris polyphylla Sm. Rhizome Extract in Cyclophosphamide-Induced Hepatic Damaged Mice: Tropical Journal of Natural Product Research (TJNPR), 7(8), 3652–3657. Retrieved from