Antibacterial Activity of Red Ginger (Zingiber officinale var. rubrum) and Black Turmeric (Curcuma caesia) Extracts as Growth Inhibitors of Klebsiella pneumonia

http://www.doi.org/10.26538/tjnpr/v7i8.14

Authors

  • Siti Juariah Faculty of Applied Sciences and Technology, Universiti Tun Hussein Onn Malaysia (UTHM), Hub Pendidikan Tinggi Pagoh, KM1, Jalan Panchor, 84600 Muar, Johor, Malaysia
  • Fazleen I.A. Bakar Faculty of Applied Sciences and Technology, Universiti Tun Hussein Onn Malaysia (UTHM), Hub Pendidikan Tinggi Pagoh, KM1, Jalan Panchor, 84600 Muar, Johor, Malaysia
  • Mohd F. A. Bakar Faculty of Applied Sciences and Technology, Universiti Tun Hussein Onn Malaysia (UTHM), Hub Pendidikan Tinggi Pagoh, KM1, Jalan Panchor, 84600 Muar, Johor, Malaysia
  • Susi Endrini Faculty of Medical, Abdurrab University Jl. Riau Ujung No. 73, Tampan, Air Hitam, Payung Sekaki, Pekanbaru, Riau 28291, Indonesia
  • Sri Kartini Faculty of Applied Sciences and Technology, Universiti Tun Hussein Onn Malaysia (UTHM), Hub Pendidikan Tinggi Pagoh, KM1, Jalan Panchor, 84600 Muar, Johor, Malaysia
  • Azman Mohamad UWG Marketing & Distributors Sdn. Bhd., Lot 7068, PT 5117, Kedai Tingkat Atas Taman D’Wanza, Kg Gong Kepas Dalam, 22200 Kampung Raja, Terengganu, Malaysia.
  • Ahmad F.M. Hanafi UWG Marketing & Distributors Sdn. Bhd., Lot 7068, PT 5117, Kedai Tingkat Atas Taman D’Wanza, Kg Gong Kepas Dalam, 22200 Kampung Raja, Terengganu, Malaysia.

Keywords:

SEM, Medicinal Plant, Infectious Disease, Dilution, Cell leakage, Antibacterial resistance

Abstract

Secondary plant metabolites play important role as potent drug candidates against antibacterialresistant pathogens such as Klebsiella pneumoniae which commonly causes pneumonia. Red ginger (Zingiber officinale var. rubrum) and black turmeric (Curcuma caesia) from the Zingiberaceae family are the potential plants as antibacterial agents. This study aimed to determine the ability of antibacterial activity and the inhibition mechanism of red ginger, black ginger and mixed (red ginger and black turmeric) extracts on the growth of K. pneumoniae. The method used was in vitro testing using the dilution method. Results showed that red ginger, black ginger and mixed ethanol extracts could inhibit K. pneumoniae growth at concentrations of 125 µg/mL and 250µg/mL, respectively, with a marked decrease in absorbance values before and after incubation. Further observations on bacterial cell leakage showed that the higher concentration of mixed ethanol extract, red ginger and black turmeric, the higher the leakage of K. pneumoniae bacterial
cells seen from the increase in absorbance values that could be captured by wavelengths of 260 nm and 280nm, respectively. Based on scanning electron microscope (SEM), the quantity of K.pneumoniae after treating black turmeric, red ginger, and mixed ethanol extracts decreased, and the cell walls became wrinkled and destroyed. Hence, ethanol extract from red ginger and black
turmeric can be recommended as an alternative natural antibacterial in inhibiting the growth of K. pneumoniae, which causes pneumonia infection.

Author Biographies

Siti Juariah, Faculty of Applied Sciences and Technology, Universiti Tun Hussein Onn Malaysia (UTHM), Hub Pendidikan Tinggi Pagoh, KM1, Jalan Panchor, 84600 Muar, Johor, Malaysia

Faculty of Pharmacy and Health Sciences, Abdurrab University Jl. Riau Ujung No. 73, Tampan, Air Hitam, Payung Sekaki, Pekanbaru, Riau 28291, Indonesia

Sri Kartini, Faculty of Applied Sciences and Technology, Universiti Tun Hussein Onn Malaysia (UTHM), Hub Pendidikan Tinggi Pagoh, KM1, Jalan Panchor, 84600 Muar, Johor, Malaysia

Faculty of Pharmacy and Health Sciences, Abdurrab University Jl. Riau Ujung No. 73, Tampan, Air Hitam, Payung Sekaki, Pekanbaru, Riau 28291, Indonesia

References

ChassagneF, SamarakoonT, PorrasG, LylesJT, Dettweiler M, MarquezL, Salam AM, Shabih S, FarrokhiDR, QuaveCL. A systematic review of plants with antibacterial activities: A taxonomic and phylogenetic perspective. Front Pharm.2020; 11, 586548.

WHO. Penumonia. [Online]. 2021 [cited 2023 May 27]. Available from: https://www.who.int/news-room/factsheets/detail/pneumonia.

Martin, Rebekah M, Michael A, Bachman. Colonization, infection, and the accessory genome of Klebsiella Pneumoniae. Front CellInfect Microbiol,2018; 8(Jan): 1-15.

Dutu LE, Popescu ML, Purdel CN, IlieEI, Luta EA, Costea L, GîrdCE. Traditional medicinal plants: a possible source of antibacterial activity on respiratory diseases induced by Chlamydia pneumoniae, Haemophilus influenzae, Klebsiella pneumoniae and Moraxella catarrhalis.Diversity, 2022; 14: 145-155.

TsereteliM, Sidamonidze K, Tsereteli D, Malania L, Vashakidze E. Epidemiology of carbapenem-resistant Klebsiella pneumoniae in intensive care units of multiprofile hospitals in Tbilisi, Georgia. Georg,Med News,2018;280(281): 164-168.

GorlenkoCL, Kiselev HY, Budanova EV, Zamyatnin AA, Jr Ikryannikova LN. Plant secondary metabolites in the battle of drugs and drug-resistant bacteria: new heroes or worse clones of antibiotics?.Antibiotics (Basel), 2020; 9(4): 170-182.

AbdurrahmanM, LestariES,Prihatiningsih T. The effectiveness of ethanol extract of Neem leaf (Azadirachta indica) mouthwash against the growth of Streptococcus sp. J BiomediTranslat Res, 2022; 8(2): 76-80.

NarayananZ, Glick BR..Secondary metabolites produced by plant bacterial endophytes. Microorganisms, 2022;10(10): 2008.

WHO. Lack of innovation set to undermine antibiotic performance and health gains [Online]. 2022 [cited 2023 May 27]. Available from:

https://www.who.int/news/item/22-06-2022-22-06-2022-lack-of-innovation-set-to-undermine-antibioticperformance-and-health-gains

Assegaf S, Kawilarang AP, Handajani R. Antibacterial activity test of red ginger extract (Zingiber officinale var. rubrum) against Streptococcus pyogenes in vitro. Biomol Health Sci J, 2020;3(1): 24-27.

Chakotiya AS, NarulaA,Dharma RK.Efficacy of methanol extract of Zingiber officinale rhizome against acute pneumonia caused by Pseudomonas aeruginosa. J Lung Health Disea, 2018; 2(1): 1-8.

ZaeandiMK, Ghale HEG, Ranjbar R. Characterization of virulence factors and antibacterial activity of curcumin in hypervirulent Klebsiella pneumoniae. Future Med,2022;17(7).

SylvesterWS, Son R, Liew KF, RukayadiY. Antibacteri activity of Java turmeric (Curcuma xanthorrhizaRoxb.) extract against Klebsiella pneumoniae isolated from several vegetables. Int Food Res J, 2015; 22(15):1770-1776.

IndrawatiI, Mia M, Isy’ainiRM. Antibacterial activity of ethanolic extracts of rhizome from three ginger varieties against acne isolated bacteria. Nusantara Biosci, 2017; 9(1),.

CiulciI. Methodology for the analysis of vegetable drugs, chemical industries branch, division of industrial operations. UNIDO Romania; 1994. 24, 26, 67p.

TankoY, Abdelaziz MM, Adelaiye AB, Fatihu MY, Musa KY. Effects of hydromethanolic leaves extract of Indigoferapulchra on blood glucose levels of normoglycemic and alloxan-induced diabetic wistar rats. Int J Appl Res Prod Nat, 2008; 1(4):13-18.

Pandey D. Antibacterial efficacy of Curcuma caesia from bastar district of chhattisgarh, india. Int J Pharm Sci,2014; 5(6): 2294-2301.

Abu Bakar MF, Ismail NA, Isha A, Mei Ling AL. Phytochemical composition and biological activities of selected wild berries (Rubus moluccanus L., R. fraxinifoliusPoir., and R. alpestris Blume). Evid Based Complementary Altern Med, 2016; 2016: 1-16.

Winnett V, Boyer H, Sirdaarta J, Cock IE. The potential of Tasmannia lanceolata as a natural preservative and medicinal agent: antimicrobial activity and toxicity. Pharmacog Com, 2014; 4(1): 42-52.

Jenie BSL, Priosoeryanto BP, Syarief R,Rekso GT. Mode of action Temukunci (Kaempferia pandurata) essential oil on E. coli K1. 1 cell determined by leakage of material cell and salt tolerance assays. HAYATI J Biosci, 2008; 15(2): 56-60.

Morgan M, Rogers C, Juan AV, Katherine BG, Anne FP, Morgan TMG, Maureen R, John PC. Cranberry (Vaccinium macrocarpon) oligosaccharides decrease biofilm formation by uropathogenic Escherichia coli. J Funct Foods, 2017; 17(12): 235-242.

Shamsudin NF, Ahmed QU, Mahmood S, Ali Shah SA, Khatib A, Mukhtar S, Alsharif M, Parveen H, Zakaria ZA. Antibacterial effects of flavonoids and their structureactivity relationship study: a comparative interpretation. Molecules, 2022; 27: 1149

Tungmunnithum D, Thongboonyou A, Pholboon A, Yangsabai A. Flavonoids and other phenolic compounds from medicinal plants for pharmaceutical and medical aspects: an overview. Medicines (Basel), 2018; 5(3): 93-102.

Kaczmarek B. Tannic acid with antiviral and antibacterial activity as a promising component of biomaterials-a minireview. Materials (Basel), 2020; 13(14), 3224.

Wojnicz D, Tichaczek-Goska D, Korzekwa K, Kicia M, Hendrich AB. Study of the impact of cranberry extract on the virulence factors and biofilm formation by Enterococcus faecalis strains isolated from urinary tract infections. Int J Food Sci Nut, 2016; 67(8): 1005-16.

Mohanan S, Nabeela R, Bimal RKS. Formulation and evaluation of antimicrobial gels for the treatment of paronychia. Int J Appl Pharmaceutics, 2018; 10(6): 161-170.

Alsheikh HMA, Sultan I, Kumar V, Rather IA, Al-Sheikh H, Tasleem JA, Haq QMR. Plant-based phytochemicals as possible alternative to antibiotics in combating bacterial drug resistance. Antibiotics (Basel), 2020: 9(8): 480.

Sartika D, Astuti S, IswandariR. Inhibitory study of cassava leather ethanol extract as natural antimicrobial in reducing Salmonella sp. and Escherichia Coli on contamination chicken meat (Gallus Domesticus). J Physics: Conf Series, 2021; 1751: 012048.

Mere KJ, Bintang M, Safithri M. Antibacterial effectiveness of Syzygiumcumini (L.) skeels leaves toEscherichia colipBR322. Indonesian J Chem Res, 2021; 9(1): 8-14.

Kowalska KB, Dudek WR. The minimum inhibitory concentration of antibiotics: methods, interpretation, clinical relevance. Pathogens, 2021; 10(2): 165.

Dosoky NS, Setzer WN. Chemical composition and biological activities of essential oils of curcuma species. Nutrients, 2018; 10: 1196.

Septama AW, Tasfiyati AN, Kristiana R, Jaisi A. Chemical profiles of essential oil from Javanese turmeric (Curcuma xanthorrhizaRoxb.), evaluation of its antibacterial and antibiofilm activities against selected clinical isolates. South Afr J Bot, 2022; 146:728-734.

Nobiola RK, Triwahyuni T, Triswanti N, WarganegaraE. Test the sensitivity of yellow turmeric and white turmeric to milk contaminating bacteria. J Ilmu Kesehatan, 2020; 1(4), 263-269.

Huang S, Chen X, Yan R, Huang M, Chen D. Isolation, identification and antibacterial mechanism of the main antibacterial component from pickled and dried mustard (Brassica juncea Coss. var. foliosa Bailey). Molecules, 2022; 27(8): 2418.

Naufalin R, Herastuti SR. Antibacterial acitvity of Nicolaia speciosa fruit extract. Int Food Res J, 2017; 24(1): 379-385.

Benfield AH, Henriques ST. Mode-of-action of antimicrobial peptides: membrane disruption vs. intracellular mechanisms. Front Med Tech, 2020; 2: 610997.

Chikada T, Kanai, T., Hayashi, M., Kasai, T., Oshima, T., &Shiomi, D. Direct observation of conversion from walled cells to wall-deficient l-form and vice versa in Escherichia coli indicates the essentiality of the outer membrane for proliferation of l-form cells. Front Microbiol, 2021;12,

GórniakI, Rafal B, KróliczewskiJ. Comprehensive review of antimicrobial activities of plant flavonoids. Phytochem Rev, 2019; 18(33):241–72.

Ita BN, Eduok SI. Antioxidant and Antibacterial Activity of Alkaloid Fractions of TristemmahirtumP. Beauv. Trop J Nat Prod Res. 2020; 4(4):179-184.

Khan MI, Ahhmed A, Shin JH, Baek JS, Kim MY, Kim JD. Green tea seed isolated saponins exerts antibacterial effects against various strains of gram positive and gram negative bacteria, a comprehensive study in vitro and in vivo. Evid Based Complementary Altern Med, 2018; 2018, 3486106.

Julianti TB, Bakar MFA, Wikantyasning ER. Phytochemical, Antioxidant Analysis and In Vitro Xanthine Oxidase Inhibitory Activity of Kaempferia parviflora and Kaempferia galanga. Trop J Nat Prod Res. 2022; 6(12):1981-1985.

Vaou N, Stavropoulou E, Voidarou C, Tsigalou C, Bezirtzoglou E. Towards advances in medicinal plant antimicrobial activity: a review study on challenges and future perspectives. Microorganisms, 2021; 9(10): 2041.

Published

2023-08-31

How to Cite

Juariah, S., Bakar, F. I., Bakar, M. F. A., Endrini, S., Kartini, S., Mohamad, A., & Hanafi, A. F. (2023). Antibacterial Activity of Red Ginger (Zingiber officinale var. rubrum) and Black Turmeric (Curcuma caesia) Extracts as Growth Inhibitors of Klebsiella pneumonia: http://www.doi.org/10.26538/tjnpr/v7i8.14. Tropical Journal of Natural Product Research (TJNPR), 7(8), 3658–3665. Retrieved from https://tjnpr.org/index.php/home/article/view/2411