Mupirocin Loaded Niosomal Gel for Topical Wound Healing Applications


  • Srikrishna Theerdhala Research Scholar, Department of Pharmaceutics, Faculty of Pharmacy, Dr.M.G.R. Educational and Research Institute, Deemed to be University, Chennai - 600077, Tamil Nadu, India.
  • Narayanaswamy Harikrishnan Department of Pharmaceutical Analysis, Faculty of Pharmacy, Dr. M.G.R. Educational and Research Institute, Deemed to be University, Chennai - 600077, Tamil Nadu, India.


Mupirocin gel, Niosomes, Nanomedicine, Wound healing


Mupirocin-loaded niosomal gel has been developed to enhance the drug deposition for a longer period at the targeted site and sustained the rate of release of the drug. A lipid hydration technique was employed to formulate niosome with polymers Carbopol and Chitosan at various concentrations. Tween 80 is a non-ionic surfactant utilized in the formulation to improve the entrapment efficiency of the drug. Cholesterol is utilized in the formulation to improve vesicle stability and glycerin is a gelling and moistening agent. In addition, to improve the stability of the niosomal gel Methylparaben is also added to the formulation.FTIR and DSC studies are used to
find out the compatibility study of the drug and other excipients. The post-evaluation studies confirm that yield percentage lies between 85 - 93%, entrapment efficiency 83 - 97%, drug content lies within the limit of 87 – 98%, pH range matches the skin pH and the obtained range is 6.25 - 7.3. Viscosity and Spreadability show the result within the limit of 410 - 560 cps and 3.8 - 5.4 gcm/s respectively. The post-evaluation study was further subjected to an in-vitro diffusion study. The formulation F5 has shown a better sustained release of active drug (98% at 12hr) which contains a higher ratio of carbopol and tween 80. A higher concentration of tween 80 increases
the entrapment efficiency of mupirocin in the niosome and carbopol helps to sustain the release rate to an optimum period as a swellable gelling agent. 

Author Biography

Srikrishna Theerdhala, Research Scholar, Department of Pharmaceutics, Faculty of Pharmacy, Dr.M.G.R. Educational and Research Institute, Deemed to be University, Chennai - 600077, Tamil Nadu, India.

Department of Pharmaceutics, Ratnam Institute of Pharmacy, Nellore - 524346, Andhra Pradesh, India.


Priya SR, Roselin RB, Karuppiah A, Sankar V. Formulation of mupirocin adsorbed silver nanoparticle with antibiofilm agents for enhancing antibacterial activity. Ind. J. Pharm. Edu. Res. 2022; 56(1): 50-57.

Mehrarya M, Gharehchelou B, Haghighi Poodeh S, Jamshidifar E, Karimifard S, Farasati Far B. Niosomal formulation for antibacterial applications. J. Drug Targeting. 2022; 30(5): 476-493.

Cosco D, Paolino D, Muzzalupo R, Celia C, Citraro R, Caponio D. Novel PEG-coated niosomes based on bolasurfactant as drug carriers for 5-fluorouracil. Biomed. Microdevices. 2009;11(5): 1115-1125.

Wu T, Zhu C, Wang X, Kong Q, Guo T, He Z. Cholesterol and phospholipid-free multilamellar niosomes regulate transdermal permeation of a hydrophobic agent potentially administrated for treating diseases in deep hair follicles. J. Pharm. Sci. 2022; 111(6): 1785-1797.

Nikam NR, Patil PR, Vakhariya RR, Magdum CS. Liposomes: A Novel Drug Delivery System: An Overview. Asian Pharm. Res. 2020; 10(1): 23-28.

Erawati T, Fitriani RD, Hariyadi DM. Topical Antimicrobial Microparticle-Based Polymeric Materials for Burn Wound Infection. Tropical Journal of Natural Product Research. 2021; 5(10):1694-1702.

Abdelkader H, Alani AW, Alany RG. Recent advances in non-ionic surfactant vesicles (niosomes): self-assembly, fabrication, characterization, drug delivery applications, and limitations. Drug delivery.2014; 21(2): 87-100.

Manosroi A, Wongtrakul P, Manosroi J, Sakai H, Sugawara F, Yuasa M. Characterization of vesicles prepared with various non-ionic surfactants mixed with cholesterol. Colloids and Surfaces B: Biointerfaces. 2003; 30: 129-138.

Ajrin M, Anjum F. Proniosome: A promising approach for vesicular drug delivery. Turk. J. Pharm.Sci. 2021; 19(4): 462-475.

Rahimpour Y, Hamishehkar H. Niosomes as a carrier in dermal drug delivery. Recent Adv. Novel Drug Carrier Sys. 2012; 1(1): 141-164.

Chen S, Hanning S, Falconer J, Locke M, Wen J. Recent advances in non-ionic surfactant vesicles (niosomes): Fabrication, characterization, pharmaceutical and cosmetic applications. Euro. J. Pharm. Biopharm. 2019; 144: 18-39.

Rajera R, Nagpal K, Singh SK, Mishra DN. Niosomes: a controlled and novel drug delivery system. Biological and Pharm. Bulletin. 2011; 34(7): 945-953.

Yasamineh S, Yasamineh P, Kalajahi HG, Gholizadeh O, Yekanipour Z, Afkhami H. A state-of-heart review on the recent advances of niosomes as a targeted drug delivery system. Int. J. Pharm. 2022; 12(1): 87-98.

Barani M, Mirzaei M, Torkzadeh-Mahani M, LohrasbiNejad A, Nematollahi MH. A new formulation of hydrophobin-coated niosome as a drug carrier to cancer cells. Mat. Sci. Eng: C. 2020; 113: 110-122.

Hakim RF, Fakhrurrazi F, Rezeki S, Sari LM, Marfirah Z. Hemostatic and Wound Healing Effects of Gracilariaverrucosa Extract Gel in Albino Rats. Tropical Journal of Natural Product Research. 2020;4(11):912-917.

Gisby J, Bryant J. Efficacy of a new cream formulation of mupirocin: comparison with oral and topical agents in experimental skin infections. Antimicrob Agents Chemother. 2000; 44: 255–260.

Coates A, Hu Y, Bax R, Page C. The future challenges facing the development of new antimicrobial drugs. Nat. Rev. Drug Discov. 2002; 1(11): 895-910.

Poovelikunnel T, Gethin G, Humphreys H. Mupirocin resistance: clinical implications and potential alternatives for the eradication of MRSA. J. Antimicrob. Chemo. 2015; 70(10): 2681-2692.

Minhas MU, Ahmad S, Khan KU, Sohail M, Abdullah O, Khalid I.Synthesis and evaluation of polyethylene glycol-4000-co-poly (AMPS) based hydrogel membranes for controlled release of mupirocin for efficient wound healing. Current drug delivery. 2022; 19(10): 1102-1115.

Sutherland R, Boon RJ, Griffin KE, Masters PJ, Slocombe B, White AR. Antibacterial activity of mupirocin (pseudomonic acid), a new antibiotic for topical use. Antimicrob.Agents Chemother. 1985; 27(4): 495-498.

Thomas CM, Hothersall J, Willis CL, Simpson TJ. Resistance to and synthesis of the antibiotic mupirocin. Nat. Rev. Microbiol. 2010; 8(4): 281-289.

Pappa KA. The clinical development of mupirocin. J Am Acad Dermatol. 1990; 22(5): 873-879.

Dowd FJ, Johnson BS, Mariotti AJ, Kumar P. Pharmacology of specific drug groups: antibiotic therapy. Pharmacol. Ther. Dent. 2017; 12(8): 457-487.

Singh AP, Gaur PK, Sharma SK, Gupta DK. Formulation and characterization of mupirocin gel and evaluation of in vitro antimicrobial activity against Staphylococcus aureus. Europ. J. Mol. Clin. Med. 2020; 7(11): 7491-7501.

Mahalakshmi S, Sankar V. In-vitro antibacterial effect of mupirocin in combination with three essential oils against Staphylococcus aureus. Int. J. Pharm. Sci. Res. 2020; 11(2): 705-709.

Ojo BO, Enwuru NV, Mendie UE. Evaluation of HoneyBased Pharmaceutical Preparations for the Management of Diabetic Wounds. Tropical Journal of Natural Product Research. 2022;6(1):109-116.

Ahmad H, Arya A, Agrawal S, Dwivedi AK. Novel lipid nanostructures for delivery of natural agents with antioxidant, anti-inflammatory and antistroke potential: perspectives and outcomes. Nanostruc. oral med. 2017; 17(6): 577-605.

Singh AP, Gaur PK, Sharma SK, Gupta DK. Formulation and characterization of mupirocin gel and evaluation of in Vitro antimicrobial activity against Staphylococcus aureus. Eur. J. Molec. & Clin. med. 2020; 7(11); 7491-7501.

Sera UV, Ramana MV. In vitro skin absorption and drug release–a comparison of four commercial hydrophilic gel preparations for topical use. The Indian Pharmacist. 2006; 73:356-360.

Acharya A, Dhakal P, Khadka D. Formulation and Evaluation of Transdermal Gel of Lornoxicam and its Delivery by Passive and Iontophoresis Method: A Comparative Study. Int. J. Pharm. Sci. Res. 2016; 7(2): 810-818.

Nematollahi MH, Pardakhty A, Torkzadeh-Mahanai M, Mehrabani M, Asadikaram G. Changes in physical and chemical properties of niosome membrane induced by cholesterol: a promising approach for niosome bilayer intervention. RSC Adv. 2017; 7(78): 49463-49472.

Bhattacharya M, Malinen MM, Lauren P, Lou YR, Kuisma SW, Kanninen L. Nanofibrillar Cellulose Hydrogel Promotes Three-Dimensional Liver Cell Culture. J. Control Release. 2012; 164(3): 291–298.



How to Cite

Theerdhala, S., & Harikrishnan, N. (2023). Mupirocin Loaded Niosomal Gel for Topical Wound Healing Applications: Tropical Journal of Natural Product Research (TJNPR), 7(8), 3676–3682. Retrieved from