HPLC-MS Analysis and evaluation of Antioxidant and Anti-Inflammatory Potential of Cinnamomum cassia Extract

http://www.doi.org/10.26538/tjnpr/v7i8.10

Authors

  • Fatima Z. Labbaci Laboratory of Clinical and Metabolic Nutrition. Faculty of Nature and Life Sciences, University of Oran 1 Ahmed Ben Bella, Algeria.
  • Hamza Belkhodja Laboratory of Bioconversion, Microbiology Engineering and Health Safety, University of Mustapha Stambouli, Mascara, 29000, Algeria.
  • Fatima Z. Elkadi Department of Biology, University of Djilali Liabes, Sidi Bel Abbes, 22000, Algeria
  • Ahmed Megharbi Faculty of science and technology, University of Relizane, Bourmadia, Relizane, 48000, Algeria.
  • Khadidja Belhouala Laboratory Research on Biological Systems and Geomatics, University of Mustapha Stambouli, Mascara, 29000, Algeria

Keywords:

HPLC-MS, Inflammation, Oxidative stress, Cinnamomum cassia

Abstract

In the current decade, growing interest has been in using herbs and spices as antioxidants and antiinflammatory agents. This study aims to investigate the chemical composition and evaluation of the antioxidant effect of Cinnamomum cassia extract. First, the chemical composition of C. cassia methanolic extract was determined using the HPLC-MS method. Then, the antioxidant potential
was evaluated using the DPPH scavenging and total antioxidant capacity (TAC) methods. For the anti-inflammatory potential, three methods were performed: Membrane stabilization assay and inhibition of protein denaturation. five compounds were identified in the methanolic extract: Quercetin-O-hexoside, Quercetin-O-pentoside, Kaempferol-O-coumaroyl, Diosmin and Cinnamic acid. The methanolic extract exhibited the highest scavenging activity recording activity of 93.08 ± 0.05% at 1000 μg/ mL concentrations compared to the aqueous extract (77.03 ± 0.07%). The methanolic extract also presented the highest value of TAC (208.85 mg AAE/g) compared to the aqueous extract (199.56 ± 0.47 mg AAE/g). Methanolic extract presented the highest percentage for HRBC protection (87.27 ± 0.09%) at concentrations of 1000 μg/ mL. For the protein denaturation method, the aqueous extract showed an inhibition percentage of 32.86 ± 4.66% against methanolic extract (24.56 ± 4.11%). Cinnamomum cassia manifested an important free radical scavenging activity and anti-inflammatory potential. Therefore, it can be considered an alternative treatment against oxidative stress and biomolecules damages. 

References

Bahmani M, Saki K, Asadbeygi M, Adineh A, Saberianpour S, Rafieian-Kopaei M, Bahmani F, Bahmani E. The effects of nutritional and medicinal mastic herb (Pistacia atlantica). J Chem Pharm Res. 2015; 7(1):646-653.

Karthik K, Kumar BRP, Priya VR, Kumar SK, Rathore RSB. Evaluation of the anti-inflammatory activity of Canthium parviflorum by the in-vitro method. Indian J Res Pharm Biotechnol. 2013; 1(5):729-731.

Heshmati J, Namazi N. Effects of Black Seed (Nigella sativa) on Metabolic Parameters in Diabetes Mellitus: A Systematic Review. Complement Ther Med. 2015; 23: 629–631.

Nabavi SF, Di Lorenzo A, Izadi M, Sobarzo-Sánchez E, Daglia M, Nabavi SM. Antibacterial Effects of Cinnamon: From Farm to Food, Cosmetic and Pharmaceutical Industries. Nutrients 2015; 7(9): 7729-7748.

Kallel I, Hadrich B, Gargouri B, Chaabane A, Lassoued S, Gdoura R, Bayoudh A, Ben Messaoud E. Optimization of Cinnamon (Cinnamomum zeylanicum Blume) Essential Oil Extraction: Evaluation of Antioxidant and Antiproliferative Effects. Evid Based Complement Alternat Med. 2019; 2019: 6498347. doi 10.1155/2019/6498347.

Vijayakumar K, Prasanna B, Rengarajan RL, Rathinam A, Velayuthaprabhu S, Vijaya Anand A. Antidiabetic and hypolipidemic effects of Cinnamon cassia bark extracts: an in vitro, in vivo, and silico approach, Arch Physiol Biochem. 2020; 126:1-11. DOI 10.1080/13813455.2020.1822415.

Kamble S, Rambhimaiah S. Antidiabetic activity of aqueous extract of Cinnamomum cassia in alloxan-induced diabetic rats. Biomed Pharmacol J. 2013; 6: 83-88.

Mahendran G, Manoj M, Murugesh E, Sathish Kumar R, Shanmughavel P, Rajendra Prasad KJ, Narmatha Bai V. In vivo antidiabetic, antioxidant and molecular docking studies of 1, 2, 8-trihydroxy-6-methoxy xanthone and 1, 2-dihydroxy-6-methoxyxanthone-8-O-β-D-xylopyranosyl isolated from Swertia corymbosa. Phytomed. 2014; 21: 1237–1248. 10.1016/j.phymed.2014.06.011.

Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through forming a phosphomolybdenum complex: specific application to determining vitamin E. Anal Biochem. 1999; 269(2): 337-341.

Shinde UA, Phadke AS, Nair AM, Mungantiwar AA, Dikshit VJ, Saraf VO. Membrane stabilizing activity—a possible mechanism of action for the anti-inflammatory activity of Cedrus deodara wood oil. Fitoterapia. 1999; 70:251–7.

Chukwuma IF, Aniagboso KT, Atrogo BE, Ese UE, Ezeali O, Eze AU, Onuorah SN, Onyishi ST, Titus BM, Ugwu EI. Elucidation of the Phytochemicals, Safety Profile, and Preclinical Anti-Inflammatory Activity of Ethanol Extract of Combretum paniculatum Leaves. Trop. J. Nat. Prod. Res. 2022; 6(12):2035-40. http://www.doi.org/10.26538/tjnpr/v6i12.23.

Hussain Z, Khan JA, Arshad MI, Muhammad F, Abbas RZ. Comparative characterization of cinnamon, cinnamaldehyde and kaempferol for phytochemical, antioxidant and pharmacological properties using acetaminophen-induced oxidative stress mouse model. Bol Latinoam Caribe Plant Med Aromat. 2021; 20(4): 339 - 350. https://doi.org/10.37360/blacpma.21.20.4.25.

Rahayu DUC, Hakim RA, Mawarni SA, Satriani AR. Indonesian Cinnamon (Cinnamomum burmannii): Extraction, Flavonoid Content, Antioxidant Activity, and Stability in the Presence of Ascorbic Acid. Cosmetics. 2022; 9: 57. https://doi.org/10.3390/cosmetics9030057.

Rue EA, Rush MD, Van Breemen R.B. Procyanidins: A comprehensive review encompassing structure elucidation via mass spectrometry. Phytochem Rev. 2018; 17: 1–16.

Ronsisvalle S, Panarello F, Longhitano G, Siciliano EA, Montenegro L, Panico A. Natural flavones and flavonols: Relationships among antioxidant activity, glycation, and metalloproteinase inhibition. Cosmetics. 2020: 7: 71.

Akkawi M, AbuLafi S, Attieh H, Abu-Remeleh Q, Makhamra S, Qutob M. Preparative HPLC fractionation of Cinnamomum cassia Water Extract and their in-vitro Antimalarial Activities. J App Pharm Sci. 2017; 7 (01): 129-134.

Wang YH, Bharathi A, Dhammika Nanayakkara NP, Zhao J, Ikhlas AK. Cassia Cinnamon is a Source of Coumarin in Cinnamon-Flavored Food and Food Supplements in the United States. J Agric Food Chem. 2013; 61: 4470−4476. dx.doi.org/10.1021/jf4005862.

Woehrlin F, Fry H, Abraham K, Preiss-Weigert A. Quantification of flavouring constituents in cinnamon: High variation of coumarin in cassia bark from the German retail market and inauthentic samples from Indonesia. J Agric Food Chem. 2010; 58:10568−10575.

Kang H, Park SH, Yun JM, Nam TG, Kim YE, Kim DO, Kim YJ. Effect of cinnamon water extract on monocyte-tomacrophage differentiation and scavenger receptor activity. BMC Complement Altern Med, 2014; 14: 90. doi 10.1186/1472-6882-14-90.

Kamaliroosta L, Gharachorloo M, Kamaliroosta Z, Alimohammad Zadeh KH. Extraction of Cinnamon Essential Oil and Identification of its Chemical Compounds. J Med Plants Res. 2012; 6(4): 609-614.

Rao PV, Gan SH. Cinnamon: A Multifaceted Medicinal Plant. Evid. Based Complement Altern Med. 2014; 2014:642942.

Attieh HA, Abu-Lafi S, Jaber S, Abu-Remeleh Q, Lutgen P, Akkawi M. Cinnamon bark water-infusion as an in-vitro inhibitor of β-hematin formation. J Med Plants Res, 2015; 9(38): 998-1005.

Das S, Gurupadayya B, Vikram HPR, Shanmukha I, Neogi A, Namitha B. Phytochemical Analysis of Quercetin, Catechol and Tannic Acid in Ethanol Extract of Barleria Prionitis Linn Leaf by RP-HPLC Technique. Trop. J. Nat. Prod. Res. 2022; 6(12):1990-9. http://www.doi.org/10.26538/tjnpr/v6i12.16.

Yang CH, Li RX, Chuang LY. Antioxidant activity of various parts of Cinnamomum cassia extracted with different extraction methods. Molecules. 2012; 17: 7294-7304.

Singh J, Parasuraman S, Kathiresan S. Antioxidant and antidiabetic activities of methanolic extract of Cinnamomum cassia. Phcog Res. 2018; 10: 237-42. DOI: 10.4103/pr.pr_162_17.

Abeysekera WPKM, Arachchige SPG, Ratnasooriya WD. Bark extracts of Ceylon cinnamon possess antilipidemic activities and bind bile acids in vitro. Evid Based Comp Altern Med. 2017; 2017: 1 - 10. https://doi.org/10.1155/2017/7347219.

Prakash B, Singh P, Yadav S, Singh SC, Dubey NK. Safety Profile Assessment and Efficacy of Chemically Characterized Cinnamomum Glaucescens Essential Oil against Storage Fungi, Insect, Aflatoxin Secretion and as Antioxidant. Food Chem Toxicol. 2013; 53: 160–167.

Eweys Aya S, Yan-Sheng Z, Osama MD. Improving Cinnamomum cassia's antioxidant and anticancer potential via fermentation with Lactobacillus plantarum. Biotechnol Rep. 2022; 36: e00768. https://doi.org/10.1016/j.btre.2022.e00768.

Wijayanti WA, Zetra Y, Burhan P. 2011. Essential Oils from Cinnamomum Stems (Cinnamon) From the Lauraceae Family as Natural Insecticides, Antibacterials and Antioxidants. Thesis. Faculty of Mathematics and Natural Sciences Sepuluh Nopember Institute of Technology.

Surabaya.

Yang L, Tan GY, Fu YQ, Feng JH, Zhang MH. Effects of acute heat stress and subsequent stress removal on the function of hepatic mitochondrial respiration, ROS production and lipid peroxidation in broiler chickens. Comparative Biochem Physiol- part C: Toxicol Pharmacol.

; 151: 204-208.

Bendary E, Francis RR, Ali HMG, Sarwat MI, El Hady S. Antioxidant and structure–activity relationships (SARs) of some phenolic and aniline compounds. Ann Agric Sci. 2013; 58: 173–181, https://doi.org/10.1016/j.aoas.2013.07.002.

Lee YH, Choo C, Watawana MI, Jayawardena N, Waisundara VY. An appraisal of eighteen commonly consumed edible plants as functional food based on their antioxidant and starch hydrolase inhibitory activities. J Sci Food Agric. 2015; 95: 2956–2964. https://doi.org/10.1002/jsfa.7039.

Varalakshmi B, Anandh AV, Prasanna R, Vijayakumar K. Antioxidant status of Cinnamomum zeylanicum linn bark. Inter J Univ Pharm Life Sci. 2012; 2: 409- 421.

Şimşek ÜG, Çiftçi M, Doğan G, Özçelik M. Antioxidant Activity of Cinnamon Bark Oil (Cinnamomum zeylanicumL.) in Japanese Quails Under Thermo Neutral and Heat Stressed Conditions. Kafkas Univ Vet Fak Derg. 2013; 19(5) : 889-894.

Moselhy SS, Junbi HH. Antioxidant properties of ethanolic and aqueous cinnamon extracts against liver injury in rats. Inter J Adv Pharm Sci. 2010; 1: 151-155.

Shalihah A, Christianty FM, Fajrin FA. Anti-inflammatory Activity of the Ethanol Extract of Cinnamon (Cinnamomum burmannii) Bark using Membrane Stabilization Method and Protein Denaturation. Indonesian J Pharm Sci Technol. 2021; 1(1): 9-14.

Suransh J, Vikram K, Pathak AN. Elucidation of in vitro antiinflammatory activity of Cinnamomum zeylanicum by HRBC membrane stabilization and protein denaturation. World J Pharm Res. 2014; 3(5): 506-512.

Oyedapo OO, Akinpelu BA, Akinwunmi KF, Adeyinka MO, Sipeolu FO. Red blood cell membrane stabilizing potentials of extracts of Lantana camara and its fractions. Inter J Plant Physiol Biochem. 2010; 2(4):46 – 51.

Olchowik E, Lotkowski K, Mavlyanov S, Abdullajanova N, Ionov M, Bryszewska M. Stabilization of erythrocytes against oxidative and hypotonic stress by tannins isolated from sumac leaves (Rhus typhina L.) and grape seeds (Vitis vinifera L.). Cell Mol Biol Lett. 2012; 17(3):333– 48.

Mittal S, Dixit PK, Gautam RK, Gupta MM. In Vitro AntiInflammatory activity of hydroalcoholic extract of Asparagus racemosus roots. Inter J Res Pharm Sci. 2013; 4(2): 203-206.

Roche J, Royer CA. Lessons from pressure denaturation of proteins. J. R. Soc. Interface. 2018; 15: 20180244.

Hong JW, Yang GE, Kim YB, Eom SH, Lew JH, Kang H. Anti-inflammatory activity of cinnamon water extract in vivo and in vitro LPS-induced models. BMC Complement Altern Med. 2012; 12: 1–8.

Lee JH, Park DH, Lee S, Seo HJ, Park SJ, Jung K, Kim SY, Kang K.S. Potential and beneficial effects of Cinnamomum cassia on gastritis and safety: Literature review and analysis of standard extract. Appl Biol Chem. 2021; 64: 1–14.

Rao P, Gan S. Cinnamon: A Multifaceted Medicinal Plant. Evidence-Based Complement Alter Med. 2014; 1-12. doi:10.1155/2014/642942.

Lee SC, Wang SY, Li CC. Anti-inflammatory effect of cinnamaldehyde and linalool from the leaf essential oil of Cinnamomum osmophloeum Kanehira in endotoxin-induced mice. J Food Drug Analysis. 2018; 26: 211-220. doi: 10.1016/j.jfda.2017.03.006.

Budiastuti, Nurcholida RD, Primaharinastiti R, Sukardiman. Anti-Inflammatory Activity of Cinnamon Bark Oil (Cinnamomum burmannii (Nees & T. Nees) Blume from Lombok Phcogj.com Timur Indonesia. Pharmacogn J. 2021;13(4): 1005-1013.

Panche AN, Diwan AD, Chandra SR. Flavonoids: an overview. J Nutr Sci.2016; 5: e47.

Attiq A, Jalil J, Husain K, Ahmad W. Raging the War Against Inflammation with Natural Products. Front Pharmacol. 2018; 9: 976.

Published

2023-08-31

How to Cite

Labbaci, F. Z., Belkhodja, H., Elkadi, F. Z., Megharbi, A., & Belhouala, K. (2023). HPLC-MS Analysis and evaluation of Antioxidant and Anti-Inflammatory Potential of Cinnamomum cassia Extract: http://www.doi.org/10.26538/tjnpr/v7i8.10. Tropical Journal of Natural Product Research (TJNPR), 7(8), 3637–3642. Retrieved from https://tjnpr.org/index.php/home/article/view/2403