Effect of Vitamin D3 on Hormonal and Histological Changes in Thyroid Gland Caused by Hyperprolactinemia of Male laboratory Rats (Rattus norvegicus)



  • Sumaya H. Sameer Biology Department, College of Education for Pure Science, University of Thi-Qar, 64001, Iraq
  • Hazar S. Saleh Biology Department, College of Education for Pure Science, University of Thi-Qar, 64001, Iraq


Phytotherapy, Plants, Moulay Yaâcoub, Morocco, Medicinal plants


Hyperprolactinemia is commonly known as abnormal levels of prolactin hormone in the blood due to endocrine disorder. The study aimed to eliminate hyperprolactinemia affecting the thyroid gland by treating it with vitamin D supplements. Eighteen male rats Rattus norvegicus were divided into three groups of six rats each : The first group received normal saline for 42 days . The second group, rats were given 5 mg/kg metoclopramide by intraperitoneal injection for hyperprolactinemia induction for 14 days. The third group, hyperprolactinemic rats received 2.5 mg/kg vitamin D3 by intraperitoneal injection for 28 days. After the end of experimental )42 days(,
hormonal parameters (prolactin hormone, Triiodothyronine hormone )T3(, and Thyroxine hormone) T4) , Thyroid stimulating hormone )TSH( were measure, and the thyroid gland was removed, processed and examine for histomorphological changes. The result of the study revealed a significant decrease (P≤0.01) in prolactin and TSH levels and a significant increase (P≤0.01) in T3 and T4 levels in Group 3 )Hyperprolactinemia group treated with vitamin D ( compared with Group 2 ) Hyperprolactinemia group(. Histological examination of sections of the thyroid gland of the group treated with vitamin D3 showed significant restoration compared to the hyperprolactinemia rats, throughout. Thyroid follicles became larger, containing dense colloidal material, in the group given Vitaim D 3 and structures of thyroid follicles. The study concluded that vitamin D3 had a protective effect on thyroid gland by stabilizing T3, T4, and TSH hormones
levels and restoration of histological architectures and thyroid follicles became larger, containing dense colloidal material, with presence normal thyrocytes remarkably. 


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How to Cite

Sameer, S. H., & Saleh, H. S. (2023). Effect of Vitamin D3 on Hormonal and Histological Changes in Thyroid Gland Caused by Hyperprolactinemia of Male laboratory Rats (Rattus norvegicus): http://www.doi.org/10.26538/tjnpr/v7i8.9. Tropical Journal of Natural Product Research (TJNPR), 7(8), 3633–3636. Retrieved from https://tjnpr.org/index.php/home/article/view/2402