Java plum (Syzygium Cumini) leaves as a potential inhibitor of SARS-CoV-2 3- Chymotrypsine-Like Protease (3CLpro)

http://www.doi.org/10.26538/tjnpr/v7i8.8

Authors

  • Diah Oktavianti Faculty of Pharmacy, Universitas Indonesia, Depok 16424, West Java, Indonesia.
  • Berna Elya Faculty of Pharmacy, Universitas Indonesia, Depok 16424, West Java, Indonesia.
  • I Gede E.P Putra Research Center of Applied Microbiology, National Agency of Research and Innovation (BRIN), Jalan Raya Bogor Km 46, Cibinong, West Java, Indonesia.
  • Is Helianti Research Center of Applied Microbiology, National Agency of Research and Innovation (BRIN), Jalan Raya Bogor Km 46, Cibinong, West Java, Indonesia.

Keywords:

Inhibition assay, Indonesia herbal, 3CLpro, SARS-CoV-2

Abstract

The 3-Chymotrypsine-Like Protease (3CLpro) is an enzyme that has a major function in the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) life cycle. It can be utilized s a protein target in search for new medications. Herbal plants are expected to have major contributions to the prevention and treatment of the 2019 Corona Virus Disease (COVID-19), as many herbal plants might have a strong affinity for 3CLpro in the treatment of COVID-19. The purpose of this study is to examine the inhibitory activity, pytochemical components, and antioxidant of extracts from nine herbal plants extracted by the Ultrasound Assisted Extraction
(UAE) method that have the potential to inhibit recombinant SARS-CoV-2 3CLpro using an in vitro method as a result, it can be used to find novel medication candidates for COVID-19 therapy. Determining antioxidant activity using DPPH and ABTS method, total polyphenol and total flavonoid content using Folin Ciocalteu and quercetin method. From nine herbal plants tested, Java Plum leaves (Syzygium cumini) had the most active inhibitory activity with an inhibitory value of 3CLpro (IC50 = 226 μg/ml) with a total polyphenol 413±1.83 mg GAE/g extract and total flavonoid as 12.091±0.037 mg QE/g extract. Measurement of antioxidant activity with the DPPH obtained an IC50 value of 3.751 ± 0.0149 μg/ml, and with an ABTS gives IC50 value 4.43±0.06μg/ml. This suggests that Java Plum leaves could be a potential source of anti-COVID.

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Published

2023-08-31

How to Cite

Oktavianti, D., Elya, B., Putra, I. G. E., & Helianti, I. (2023). Java plum (Syzygium Cumini) leaves as a potential inhibitor of SARS-CoV-2 3- Chymotrypsine-Like Protease (3CLpro): http://www.doi.org/10.26538/tjnpr/v7i8.8. Tropical Journal of Natural Product Research (TJNPR), 7(8), 3626–3632. Retrieved from https://tjnpr.org/index.php/home/article/view/2401

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