The Effect of Extraction Method on Flavonoid Content and Antioxidant Activity of Red Betel and Green Betel Extracts http://www.doi.org/10.26538/tjnpr/v7i8.7
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Abstract
Red betel (Piper crocatum Ruiz & Pav) and green betel (Piper betle L.) are traditionally used as medicines. Betel leaf contains flavonoid compounds with antihypertensive, antioxidant, and antibacterial potential. This study aimed to determine the effect of the extraction method on the total flavonoid content of the ethanol extract of red and green betel leaves using UV-Vis spectrophotometry. The samples were extracted by sonication, reflux, and Soxhlet methods, respectively, to obtain crude extracts of each species. The levels of flavonoids and their antioxidant activity were determined UV-Visible spectrophotometry. The results showed that the total flavonoid content of red betel leaves in the sonication, reflux, and Soxhlet methods were 11.4 ± 0.31, 16.1 ± 0.42, and 11.8 ± 0.05, respectively, and in green betel leaf, were10.9 ± 0.11, 6.4 ± 0.14, and 17.6 ± 0.09 mgEQ/g, respectively. The results of antioxidant activity testing using the
DPPH, ABTS, and FRAP methods showed that green betel extract has significant antioxidant activity compared to red betel extract. Extraction of green betel leaves by reflux method gave the highest total flavonoid content and correlated with its activity as an antioxidant. This study can provide information regarding the effect of the extraction method on total flavonoid content and antioxidant activity of red and green betel extracts to serve as a basis for further research development.
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References
Luliana S, Riza H, Indriyani EN. The Effect of Extraction Method on Total Phenolic Content and Antioxidant Activity of Salam Leaves (Syzygium polyanthum) using DPPH (1,1-Diphenyl-2-Picrylhidrazil). Trad Med J. 2019; 24: 72.
Shah SK, Garg G, Jhade D, Patel N. Piper Betle: Phytochemical, Pharmacological and Nutritional Value in Health Management. Int J Pharm Sci Rev Res. 2016; 38: 181–189.
Salehi B, Zakaria ZA, Gyawali R, Ibrahim SA, Rajkovic J, Shinwari ZK, Khan T, Sharifi-Rad J, Ozleyen A, Turkdonmez E, Valussi M, Tumer TB, Monzote Fidalgo L, Martorell M, Setzer WN. Piper Species: A Comprehensive Review on Their Phytochemistry, Biological Activities and
Applications. Molecules. 2019; 24: 1364.
Suri MA, Azizah Z, Asra R. A Review: Traditional Use, Phytochemical and Pharmacological Review of Red Betel Leaves (Piper Crocatum Ruiz & Pav). Asian J Pharm Res Dev. 2021; 9: 159–163.
Hamed YS, Abdin M, Akhtar HMS, Chen D, Wan P, Chen G, Zeng X. Extraction, purification by macrospores resin and in vitro antioxidant activity of flavonoids from Moringa olifera leaves. S Afr J Bot. 2019; 124. doi:10.1016/j.sajb.2019.05.006.
Panche AN, Diwan AD, Chandra SR. Flavonoids: an overview. J Nutr Sci. 2016;5:e47
Cao X, Wang L, Liu M, Ren X, Han T, Deng Y. Study on Solubilization and Stabilization of Eight Flavonoids by 17 Chinese Herbal Polysaccharides. J Chem. 2020; 1–10.
Nguyen VT, Nguyen MT, Tran QT, Thinh PV, Bui LM, Le THN, Le VM, Linh HTK. Effect of extraction solvent on total polyphenol content, total flavonoid content, and antioxidant activity of soursop seeds ( Annona muricata L.). IOP Conf Ser: Mater Sci Eng. 2020; 736: 022063.
Seno DSH, Sembiring DPDB, Kurniatin PA, Nurcholis W. Total Phenolic, Total Flavonoid Contents, and Radical Scavenging Activity of Different Concentrations of Ethanol Extract of Pyrrosia Piloselloides (L) M. G. Trop J. Pharm Res. 2022, 6, 484-487.
Nurcholis W, Alfadzrin R, Izzati N, Arianti R, Vinnai BÁ, Sabri F, Kristóf E, Artika IM. Effects of Methods and Durations of Extraction on Total Flavonoid and Phenolic Contents and Antioxidant Activity of Java Cardamom (Amomum compactum Soland Ex Maton) Fruit. Plants. 2022;
: 2221.
Akinmoladun AC, Falaiye OE, Ojo OB, Adeoti A, Amoo ZA, Olaleye MT. Effect of extraction technique, solvent polarity, and plant matrix on the antioxidant properties of Chrysophyllum albidum G. Don (African Star Apple). Bull Natl Res Cent. 2022; 46: 40.
Hua Liu, Yinghua Song, Xiaojuan Zhang. Determination of Total Flavonoids in Leek by AlCl3 Colorimetric Assay. Chem Eng Trans. 2017; 59: 775–780.
Nur S, Sami FJ, Marwati M, Nursamsiar N, Fadri A, Khairuddin K. Phenolic and Flavonoid Content of Black Mulberry (Morus nigra L.) Stem and Their Evaluation Antioxidant and Cytotoxic Profile. Borneo J Pharm. 2022; 5: 384–395.
Nur S, Wierson Y, Yulia, Sami FJ, Megawati M, Aisyah AN, Marwati M, Gani SA. Characterization, Antioxidant and α-Glucosidase Inhibitory Activity of Collagen Hydrolysate from Lamuru (Caranx ignobilis) Fishbone. JSM. 2021; 50: 2329–2341.
Yanchev N, Petkova N, Ivanov I, Delev D. Total Polyphenolic Content and Antioxidant Activity of Different Extracts from Sideritis scardica. Trop J. Pharm Res. 2022; 6(7), 1113–1118
Zhang QW, Lin LG, Ye WC. Techniques for extraction and isolation of natural products: a comprehensive review. Chin Med. 2018;13:20.
Shah SK, Garg G, Jhade D, Patel N. Piper Betle: Phytochemical, Pharmacological and Nutritional Value in Health Management. Int J Pharm Sci Rev Res.2016; 38: 181–189.
Albu A, Radu-Rusu R-M, Simeanu D, Radu-Rusu C-G, Pop IM. Phenolic and Total Flavonoid Contents and Physicochemical Traits of Romanian Monofloral Honeys. Agriculture.2022; 12: 1378.
Ramos RTM, Bezerra ICF, Ferreira MRA, Soares LAL. Spectrophotometric Quantification of Flavonoids in Herbal Material, Crude Extract, and Fractions from Leaves of Eugenia uniflora Linn. Pharmacognosy Res. 2017;9(3):253-260
Nur S, Aswad M, Yulianti R, Sami FJ, Burhan A, Fadri A.Antioxidant activity profile of extract and fraction of kersen (Muntingia calabura L.) fruits prepared by different methods. IOP Conf Ser: Earth Environ Sci. 2022; 976: 012066.
Nur S, Mubarak F, Jannah C, Winarni DA, Rahman DA, Hamdayani LA. Total phenolic and flavonoid compounds, antioxidant and toxicity profile of extract and fractions of paku atai tuber (Angiopteris ferox Copel). Food Res. 2019; 3: 734–740.
Xiao F, Xu T, Lu B, Liu R. Guidelines for antioxidant assays for food components. Food Front. 2020; 1: 60–69.
Gulcin İ. Antioxidants and antioxidant methods: an updated overview. Arch Toxicol.2020; 94: 651–715.
Prayitno SA, Kusnadi J, Murtini. ES. Antioxidant Activity of Red Betel Leaves Extract (Piper crocatum Ruiz & Pav.) By Difference Concentration of Solvents. Res J Pharm Biol Chem Sci.2016; 7: 1836–1843.
Apak R, Özyürek M, Güçlü K, Çapanoğlu E. Antioxidant Activity/Capacity Measurement. 1. Classification, Physicochemical Principles, Mechanisms, and Electron Transfer (ET)-Based Assays. J Agric Food Chem. 2016; 64: 997–1027.
Kontoghiorghes G, Kontoghiorghe C. Iron and Chelation in Biochemistry and Medicine: New Approaches to Controlling Iron Metabolism and Treating Related Diseases. Cells. 2020; 9: 1456.
Nur S, Setiawan H, Hanafi M, Elya B. Phytochemical composition, antioxidant, in vitro and in silico studies of active compounds of Curculigo latifolia extracts as promising elastase inhibitor. Saudi J Biol Sci. 2023;30(8):103716.
Yamin Y, Sabarudin S, Zubaydah WOS, Sahumena MH, Arba M, Elnawati E, Andriani R, Suryani S. Determination of Antiradical Activity, Total Phenolic and Flavonoid Contents of Kamena-mena (Clerodendrum paniculatum L) Leaves. Trop J. Pharm Res. 2021;5(2), 287–293.