Isolation and Characterization of Thermophilic Bacteria from Ikogosi Warm Spring, Ekiti State, Nigeria


  • Folashade T. Otitolaye Department of Biological Sciences, College of Science and Technology, Covenant University, Canaanland, PMB 1023 Ota, Ogun State, Nigeria
  • Angela O. Eni Department of Biological Sciences, College of Science and Technology, Covenant University, Canaanland, PMB 1023 Ota, Ogun State, Nigeria


Castenholz TYE medium, Geobacillus spp, Geothermal springs, Ikogosi, Thermophilic bacteria


Thermophilic microorganisms are adapted to thrive at high temperatures and are sources of thermostable enzymes with several potential industrial applications. The aim of this study was to isolate, characterize, and identify thermophilic bacteria from Ikogosi warm spring in Ekiti state, Nigeria. Water, sand, and rock mattings samples were obtained from different sites of the spring in sterilized vials and transported in an ice chest to the laboratory for analysis. Then, 1.5 mL of water and 1 g of each sediment were cultured on compounded Castenholz Tryptone-Yeast Extract medium at 70oC for 96 h for bacteria isolation. The study yielded twenty- seven thermophilic bacteria, which were identified based on morphological and biochemical characteristics. Results showed that out of the 27 isolates, 15% were Gram-negative organisms, and 85% were Gram-positive organisms. Based on morphological and various biochemical characteristics, the 27 isolates were closely related to the genera Thermus and Geobacillus. Further studies will screen the isolated bacteria for their ability to produce various enzymes. 

Author Biography

Angela O. Eni, Department of Biological Sciences, College of Science and Technology, Covenant University, Canaanland, PMB 1023 Ota, Ogun State, Nigeria




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How to Cite

T. Otitolaye, F., & O. Eni, A. (2021). Isolation and Characterization of Thermophilic Bacteria from Ikogosi Warm Spring, Ekiti State, Nigeria: Tropical Journal of Natural Product Research (TJNPR), 5(1), 126–129. Retrieved from