Isolation and Characterization of Thermophilic Bacteria from Ikogosi Warm Spring, Ekiti State, Nigeria
doi.org/10.26538/tjnpr/v5i1.17
Keywords:
Castenholz TYE medium, Geobacillus spp, Geothermal springs, Ikogosi, Thermophilic bacteriaAbstract
Thermophilic microorganisms are adapted to thrive at high temperatures and are sources of thermostable enzymes with several potential industrial applications. The aim of this study was to isolate, characterize, and identify thermophilic bacteria from Ikogosi warm spring in Ekiti state, Nigeria. Water, sand, and rock mattings samples were obtained from different sites of the spring in sterilized vials and transported in an ice chest to the laboratory for analysis. Then, 1.5 mL of water and 1 g of each sediment were cultured on compounded Castenholz Tryptone-Yeast Extract medium at 70oC for 96 h for bacteria isolation. The study yielded twenty- seven thermophilic bacteria, which were identified based on morphological and biochemical characteristics. Results showed that out of the 27 isolates, 15% were Gram-negative organisms, and 85% were Gram-positive organisms. Based on morphological and various biochemical characteristics, the 27 isolates were closely related to the genera Thermus and Geobacillus. Further studies will screen the isolated bacteria for their ability to produce various enzymes.
References
Vitorino L and Bessa L. Microbial Diversity: The Gap between the Estimated and the Known. Divers. 2018;10(2):46.
Merino N, Aronson HS, Bojanova DP, Feyhl-Buska J, Wong ML, Zhang S, Giovannelli, D. Living at the Extremes: Extremophiles and the Limits of Life in a Planetary Context. Front Microbiol. 2019; 10:780.
Zaccaria M, Dedrick S, Momeni B. Modeling microbial communities: a call for collaboration between experimentalists and theorists. Processes 2017; 5:53.
Horiike T, Miyata D, Hamada K, Saruhashi S, Shinozawa T, Kumar S, Chakraborty R, Komiyama T, Tateno Y.
Phylogenetic construction of 17 bacterial phyla by new method and carefully selected orthologs. Gene 2009;
:59-64.
Sharma N, Vyas G, Pathania S. Culturable Diversity of Thermophilic Microorganisms Found in Hot Springs of
Northern Himalayas and to Explore Their Potential for Production of Industrially Important Enzymes. Sch Acad J Biosci. 2013; 1:165-178.
Gupta M. Isolation & characterization of Thermus aquaticus and production of Taq DNA polymerse enzyme (PhD) Dr. Yashwant Singh Parmar University of Horticulture and Forestry Himachal Pradesh India. 2011.
Tang J, Liang Y, Jiang D, Li L, Luo Y, Shah MMR, Daroch M. Temperature-controlled thermophilic bacterial communities in hot springs of western Sichuan. China BMC Microbiol. 2018; 18:134.
Wolella EK and Tilahun B. Isolation and characterization of extremophiles from Shalla/Abidjata hot springs, Ethiopia. Acta Sci Microbiol. 2020; 3:1-5.
Mohammad BT, Al daghistani HI, Jaouani A, Abdel-latif S, Kennes C. Isolation and characterization of thermophilic
bacteria from Jordanian hot springs: Bacillus licheniformis and Thermomonas hydrothermalis isolates as potential
producers of thermostable enzymes. Int J Microbiol. 2017; 2017:1-12.
Msarah MJ, Ibrahim I, Hamid AA, Aqma WS. Optimisation and production of alpha amylase from thermophilic Bacillus spp. and its application in food waste biodegradation. Heliyon 2020; 6(6):e04183.
Khiyami MA, Serour EA, Shehata MM, Ali H, Bahklia, AH. Thermo-aerobic bacteria from geothermal springs in
Saudi Arabia. Afr J Biotechnol. 2012; 11:4053-4062.
Lele OH and Deshmukh PV. Isolation and characterization of thermophilic Bacillus sp. with extracellular enzymatic
activities from hot spring of Ganeshpuri, Maharashtra, India. Int J Appl Eng Res. 2016; 2:427-430.
Ikudayisi A, Adeyemo F, Adeyemo J. Chemical and hydrogeologic analysis of Ikogosi warm spring water in Nigeria. Int J Geol Environ Eng. 2015; 9:126-130.
Fasesan D, Dawkins K, Ramirez R, Rasheed-Jada H, Onilude A, Nash O, Esiobu N. Analysis of a Tropical Warm
Spring Microbiota Using 16S rRNA Metabarcoding. Ad Appl Microbiol. 2020; 10:145-165.
Ayodele AY, Yusuf GT, Agboola JO, Oladele PO. Harnessing the geothermal properties of Ikogosi warm
spring for power generation for entrepreneurship development. Int J Mech Eng Technol. 2019; 10:250-257.
Olowookere A, Olusayo O, Ayeni AO, Imole PO. Respondent's perception on demographic and socioeconomic characteristics of Ikogosi warm spring for environmental sustainability. Int J Sociol Anthropol. 2018;
:64-73.
Kukoyi IA, Tijani NO, Adedara MT. Evaluation of Ikogosi Warm Spring; A Potential Geotourist Site in Ekiti State,
Southwest, Nigeria. Eur J Tour Res. 2013; 1(3):1-9.
Talabi AO. Hydrogeochemistry and Stable Isotopes (δ18O and δ2H) Assessment of Ikogosi Spring Waters. Am J
Water Resour. 2013; 1(3):25-33.
Castenholz RW. Thermophilic blue-green algae and the thermal environment. Bacteriol Rev. 1969; 33:476-504.
Anderson D, Ferreras E, Trindade M, Cowan D. A novel bacterial Water Hypersensitivity-like protein shows in vivo
protection against cold and freeze damage. FEMS Microbiol Lett. 2015; 362:110.
Nshimiyimana JB, Khadka S, Mwizerwa EM, Akimana N, Adhikari S, Nsabimana A. Thermophiles: Isolation,
Characterization and Screening for Enzymatic Activity. Biosci discov. 2018; 9:430-437.
Albuquerque L, Rainey FA, Da Costa MS. The Family Thermaceae Bergey’s Manual of Systematics of Archaea
and Bacteria. (4th ed.). New Jersey: Wiley. 2018; 1–39 p.
Ghilamicael AM, Budambula NLM, Anami SE, Mehari T, Boga HI. Evaluation of prokaryotic diversity of five hot
springs in Eritrea. BMC Microbiol. 2017; 17:203.
Published
How to Cite
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
