Microbial Profile and Antibiotic Susceptibility Patterns of Microbes Isolated from Wash Hand Basins in Covenant University doi.org/10.26538/tjnpr/v5i1.16
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Abstract
Although, wash-hand basins aid in the disposal of washouts from contaminated hands, unsanitised basins can be a source of cross-contamination. The aim of this study was to analyse the microbial profiles and antibiotic susceptibility patterns of isolates from wash-and basins in selected areas in Covenant University. A total of 70 swab samples were collected from wash-hand basins in the laboratories, cafeteria, and lavatories around the hostels and classroom areas. The isolates were identified based on their colonial, microscopic, and biochemical haracteristics. The antibiotic susceptibility of selected isolates was determined using the Kirby-Bauer disk diffusion method. A total of seven bacteria and nine fungal isolates were obtained from the swab samples. The most occurring bacteria and fungi were Staphylococcus aureus (100%) and Aspergillus sp. (88%), respectively. Escherichia coli and Salmonella sp. were isolated from the wash-basins in the lavatories in the students’ hostel. A 100% sensitivity to amoxicillin and amoxicillin-clavulanic acid was observed in the enteric bacteria. Pathogenic Staphylococcus sp. was sensitive to Amoxicillin-clavulanic acid, cefuroxime, and cloxacillin. Wash-hand basins could serve as a reservoir for the potential transmission of antibiotic-resistant organisms and may be of public health concern.
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WHO 2020. Advice for the public [Internet]. [cited 2020 Aug 1]. Available from: https://www.who.int/emergencies/diseases/novelcoronavirus-2019/advice-forpublic?gclid=EAIaIQobChMI6YmP6qD16gIVArTtCh21x
wgXEAAYASAAEgKTN_D_BwE
Wolf J, Hunter PR, Freeman MC, Cumming O, Clasen T, Bartram J, et al. Impact of drinking water, sanitation and handwashing with soap on childhood diarrhoeal disease: updated meta-analysis and meta-regression. Trop Med Int Heal. 2018; 23(5):508-525.
Otter JA, Yezli S, Salkeld JAG, French GL. Evidence that contaminated surfaces contribute to the transmission of hospital pathogens and an overview of strategies to address contaminated surfaces in hospital settings. Am J Infect Contr. 2013: S6-11.
Suleyman G, Alangaden G, Bardossy AC. The Role of Environmental Contamination in the Transmission of Nosocomial Pathogens and Healthcare-Associated Infections. Current Infect Dis Reports. Curr Med Group LLC 1; 2018; 20:1-11.
Mkrtchyan HV, Russell CA, Wang N, Cutler RR. Could Public Restrooms Be an Environment for Bacterial Resistomes? Schlievert PM, editor. PLoS One. 2013; 17:8(1):e54223.
Yeasmin F, Luby SP, Saxton RE, Nizame FA, Alam M, Dutta NC, Masud A, Yeasmin D, Layden A, Rahman H, Abbott R, Unicomb L, Winch P. Piloting a low-cost hardware intervention to reduce improper disposal of solid waste in communal toilets in low-income settlements in
Dhaka, Bangladesh. BMC Public Health 2017; 17: 682.
Solomon O and Olatile T. Microbial profiles of Hands Foods Easy contact surfaces and Food contact surfaces [Internet]. Available from:
https://www.academia.edu/34589736/Microbial_profiles_of_Hands_Foods_Easy_contact_surfaces_and_Food_contact_surfaces [cited 2020 Aug 1].
Holt JH, Krieg NR, Sneath PH , Staley JT, Williams ST. Bergey’s manual of determinative bacteriology ninth edition. Eur J Paediatr Neurol. 1994; 13(6):560.
Omar-Zahid LA. Atlas Food Microbiol LAB. 2013. 1-76 p.
Ellis D, Davis S, Alexiou H, Handke R, Bartley R. Descriptions of medical fungi. North. 2007. 1-198 p.
Clinical and Laboratory Standards Institute. 2018. Performance Standards for Antimicrobial Disk Susceptibility Tests. 13th ed. CLSI standard M02. Clinical and Laboratory Standards Institute. Wayne, PA..
Majolagbe ON, Adewoyin AG, Ogunmoyewa T, Abejide MA. Antibiotic Resistance Profile of Microbial Isolates of Toilet-Bowl of Some Students’ Hostels in Ogbomoso, Nigeria. Eur J Appl Sci. 2013; 5(3):76-79.
Suen LKP, Siu GKH, Guo YP, Yeung SKW, Lo KYK, O’Donoghue M. The public washroom - Friend or foe? An observational study of washroom cleanliness combined with microbiological investigation of hand hygiene facilities. Antimicrob Resist Infect Contr. 2019; 8(1):47.
Tetteh-Quarcoo PB, Anim-Baidoo I, Kwaku AS, AbdulLatif BB, Opintan JA, Minamor AA, et l. Microbial Content of (Bowl Water) Used for Communal Handwashing in Preschools within Accra Metropolis, Ghana. Int J Microbiol. 2016; 2016:2617473.
Norsworthy AN and Pearson MM. From Catheter to Kidney Stone: The Uropathogenic Lifestyle of Proteus mirabilis.Trends in Microbiol. 2017; 25:304-315.
Mkrtchyan HV, Russell CA, Wang N, Cutler RR. Could Public Restrooms Be an Environment for Bacterial Resistomes? PLoS One 2013; 8(1):e54223.
Lee BY, Singh A, David MZ, Bartsch SM, Slayton RB, Huang SS, Zimmer SM, Potter MA, Macal CM, Lauderdale DS, Miller LG, Daum RS. The economic burden of community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA). Clin Microbiol Infect. 2013;19(6):528-536.
Ayepola OO, Olasupo NA, Egwari LO, Schaumburg F. Characterization of panton–valentine leukocidin-positive staphylococcus aureus from skin and soft tissue infections and wounds in Nigeria: A cross-sectional study F1000Research. 2018; 7(0):1-10