Bergenin Improves Antioxidative System in Tert-butyl Hydroperoxide-Induced Oxidative Stress in Mice
doi.org/10.26538/tjnpr/v5i1.14
Keywords:
Antioxidant enzymes, Bergenin, Glutathione homeostasis, Lipid peroxidation, Tert-butyl hydroperoxideAbstract
Tert-butyl hydroperoxide (TBHP) is a pro-oxidant implicating oxidative stress due to excessive formation of free radicals from hepatic metabolism via CYP2E1 and glutathione peroxidase glutathione reductase system. Bergenin is a C-glucoside derivative of gallic acid which has been claimed to exert antioxidant, anti-inflammatory, and hepatoprotective activities. The present study aimed to investigate the antioxidative effect of bergenin on TBHP-induced hepatic oxidative stress in mice. Male ICR mice received TBHP (18 mg/kg/day, intraperitoneal) with bergenin (10, 50, or 250 mg/kg/day, per oral) or gallic acid (100 mg/kg/day, per oral) for 7consecutive days. Mouse plasma was investigated for the levels of liver injury and oxidative stress markers. Mice livers were examined for histopathology, antioxidant enzyme activity, glutathione profile, and mRNA expression of antioxidant enzymes and Cyp2e1 expression. Bergenin and gallic acid attenuated TBHP-induced hepatic oxidative stress through reduction of prominent histopathological markers, including nuclear pyknosis and necrotic areas, decreases in plasma aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, and reactive oxygen species levels, and inhibition of lipid peroxidation in the plasma and livers. Moreover, both bergenin and gallic acid restored the hepatic activities of antioxidant enzymes, namely superoxide dismutase, catalase, and glutathione peroxidase, improved glutathione homeostasis, and returned mRNA expression of the hepatic antioxidant enzymes and Cyp2e1 to normal. Bergenin exhibited hepatoprotective/antioxidant effects comparable to gallic acid in the TBHP-induced oxidative stress in mice. Therefore, bergenin is a beneficial antioxidant that can improve the hepatic antioxidative system.
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