Volatile Oil Constituents, Bioactivity and Formulations of Essential Oil from Psidium guajava http://www.doi.org/10.26538/tjnpr/v7i7.40
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Abstract
With the availability of myriad of plants having insecticidal activity, the issue of toxicity while using synthetic compounds to eradicate pests would be resolved. Therefore, the search for and formulations of insecticidal plants have grown remarkably, in recent years. It is on this premise that this work sought to characterize, evaluate the bioactivity and formulations of essential oil of Psidium guajava (PG) for insecticidal activity. Volatile oil of from PG was characterized using Gas Chromatography-Mass Spectrometry to identify the compounds present. Filter paper and antifeedant methods were used to test the oils for insecticidal activities against adult Callosobruchus maculatus. The releasing profiles of the volatile oil formulations were observed for 15 days at 3 days interval using bentonite and kaolin as carriers at different concentrations (10, 20 30 40 and 50 µl). Antimicrobial activities of the volatile oil were also carried out on some bacterial and fungus pathogens using standard methods. From the results, forty-nine (49) compounds were identified in PG volatile oil with a-Limonene (29.55 %), ?- cadinene (9.61 %), caryophyllene (9.44 %), nerolidol (7.19 %) and viridiflorol (4.29 %) as major components. After 6 hours and 24 hours of filter paper and anti-feedant test, the volatile oil toxicity results showed that the percentage mortality of Callosobruchus maculatus increased significantly (p > 0.05) with increase in concentration. Oil formulation results showed that the efficacies and the insecticidal activity decreased with time. Antimicrobial studies showed that the extracted oil inhibited the growth of tested organisms at different zones of inhibition.
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References
Carson R. Silent Spring. Cambridge, MA: Riverside Press, 1962; 67-81.
Gyawali K. Pesticide Uses and its Effects on Public Health and Environment. J. Health Prom. 2018; 6:28-36.
Nathan SS. A Review of Resistance Mechanisms of Synthetic Insecticides and Botanicals, Phytochemicals, and Essential Oils as Alternative Larvicidal Agents Against
Mosquitoes. Front Physiol. 2019; 10:1591.
Abbasi E, Vehedi M, Bagheri M, Gholizadeh S, Alipour H, Moemenbellah-Fard MD. Monitoring of synthetic insecticides resistance and mechanisms among malaria
vector mosquitoes in Iran: A systematic review. Heliyon. 2022; 8(1):e08830.
Naeun R, Bass C, Feyereisen R, Vontas J. The Role of Cytochrome P450s in Insect Toxicology and Resistance. Ann Rev Entom. 2022; 67:105-24.
Oke DG, Faboro EO, Olanrewaju AA, Oyeneyin OE, Lajide L. In vitro Antifungal and In silico Antibacterial Evaluations of Anacardic Acid and its Complexes from Cashew Nut Shell
Oil. Trop J. Nat Prod Res. 2022; 6(8):1290-1296.
Falodun A, Siraj R, Choudhary MI. GC-MS analysis of insecticidal leaf essential oil of Pyrenacantha staudtii Hutch and Dalz (Icacinaceae). Trop J Pharm Res. 2009; 8(2):139-
Krishnaiah D, Sarbatly R, Nithyanandam R. A review of the antioxidant potential of medicinal plant Species. Food Bio Prod Proc. 2011; 89:217-233.
Okolie NP, Falodun A, Oluseyi D. Evaluation of the antioxidant activity of root extract of pepper fruit (Dennetia tripetala), and its potential for the inhibition of Lipid
peroxidation. Afr J. Trad Compl Altern Med. 2014; 11(3):221-227.
Misnia N, Nora ZM, Ahmad R. Microencapsulation of Citrus Grandis Peel Oil Using Interfacial Precipitation Chemistry Technique for Repellent Application. Iran J. Pharmaceut
Res. 2019; 18(1):198-209.
Ogunwande I, Olawore N, Adeleke K, Ekundayo O, Koenig W. Chemical composition of the leaf volatile oil of Psidium guajava L. growing in Nigeria. Flav Frag J. 2003; 18:136 -
Prabodh S, Prajwal P, Bimala L, William NS. Leaf essential oil composition and bioactivity of Psidium guajava from Kathmandu, Nepal. Amer J Essen Oils Nat Prod. 2015;
(2):11-14.
Khadiri A, ElMokni R, Almeida C, Nogueira JM, Araújo ME. Chemi cal composition of essential oil of Psidium guajava L. growing in Tunisia. Ind Crops Prod. 2014; 52:29-31.
Arain A, Sherazi ST, Mahesar S. Essential Oil from Psidium guajava Leaves: An Excellent Source of ß-Caryophyllene. J Nat Prod Comm. 2019; 14(4):1934-1984.
DaSilva JD, Luz AI, da Silva MH. Essential oils of the leaves and stems of four Psidium spp. Flavour Fragr J. 2003; 18(3):240–243.
Chen M, Sheu L, Chung-May W. Chemical Composition of the Leaf Essential Oil of Psidium guajava L. from Taiwan, J Essen Oil Res, 2007; 19(4):345-347.
Sacchetti G, Maietti S, Muzzoli M, Scaglianti M, Manfredini S, Radice M, Bruni R. Comparative evaluation of 11 essential oils of different origin as functional antioxidants, antiradicals
and antimicrobials in foods. Food Chem, 2005; 91:621-632.
Ofuya TI, Lale NES. Pest of stored cereals and pulses in Nigeria. Dave Collins publications, Nigeria, Pests of stored cereals and pulses in Nigeria: biology, ecology and control.
; 59-94.
Ukeh DA, Oku EE, Udo IS, Nta AI, Ukeh JA. Insecticidal effect of fruits extract from xylopia aethiopical and Dennettia (Annonaceae) against Sitophilus oryzae. Chil J. Agricul Res.
; 72(2):195-200.
Olonisakin A, Ajayi FA, Atolaiye B, Faboro EO. Controlled release profile of essential oil formulation using gas chromatography. Int J. Chem Sci. 2008; 1:43-50.
Olonisakin A, Oladimeji MO, Lajide L. Composition and antibacterial activity of stem distilled oil from xylopia aethiopica and syzgium aromaticum. J. Engin Appl Sci.
; 2:236-240.
Willey JM, Sherwood LM, Woolverton CJ. Prescott, Harley, and Klein s Microbiology, NY, 7th Edition, McGraw Hill. New York. 2008; 1050-1123.
Cole RA, Setzer WN. Chemical composition of the leaf essential oil of Psidium guajava from Monteverde, Costa Rica. J. Essen Oil Bear Plants. 2007; 10:365-373.
Santos FA, Rao VSN, Silveira ER. Investigations on the antinociceptive effect of Psidium guajava leaf essential oil and its major constituents. Phytother Res, 1998; 12:24-27.
Pino J, Agüero J, Marbot R, Fuentes V. Leaf Oil of Psidium guajava L. from Cuba. The J. Essen Oil Res. 2011; 13:61-62.
Olonisakin A, Abugan A, Akinnifesi T. Volatile oil composition and bioactivity of hyptissuaveolens (l) seeds. Trends Sci Tech J. 2017; 2(2):716–719.
Shrestha S, Poudel A, Satyal P, Dosoky NS, Chhetri BK, Setzer WN. Chemical composition and biological activity of the leaf essential oil of Callistemon citrinus from Nepal.
Amer J. Ess Oils Nat Prod. 2015; 2(5):29-33.
Sagrero-Nieves L, Bartley JP, Provis-Schwede A. Supercritical fluid extraction of the volatile components from the leaves of Psidium guajava L. (guava). Flav Frag J. 1994;
:135-137.
Siani AC, Souza MC, Henriques MG. Anti-inflammatory activity of essential oils from Syzygium cumini and Psidium guajava. Pharm Biol. 2013; 51:881-887.
El-Ahmady SH, Ashour ML, Wink M. Chemical composition and anti-inflammatory activity of the essential oils of Psidium guajava fruits and leaves. J. Essen Oil Res.
; 25:475-481.
Sanches NR, Garcia CD, Schiavini MS, Nakamura CV, Dias Filho BP. An evaluation of antibacterial activities of Psidium guajava (L.). Braz Archiv Bio Tech, 2005; 48(3):429-436.
Nascimento GF, Locatelli J, Freitas PC., Silva GL. Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistant bacteria. Braz J. Microbio. 2000;
(4):247-256.
Rathish N, Chanda S. In-vitro antimicrobial activity of Psidium guajava L. leaf extracts against clinically important pathogenic microbial strains. Braz J. Microbio.
; 38:452-458.
Priya M, Joseph B. In vitro antimicrobial activity of psidium guajava leaf essential oil and extracts using agar well diffusion method. Int J. Curr Pharmaceut Res. 2010; 2: 28-